Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Induction of DNA Damage in L5178Y Cells Treated with Gold Nanoparticle

  • Kang, Jin-Seok (Department of Biomedical Laboratory Science, Namseoul University) ;
  • Yum, Young-Na (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Joo-Hwan (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Song, Hyun-A (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Jeong, Jin-Young (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, and Nanobiotechnology Major, Korea University of Science and Technology) ;
  • Lim, Yong-Taik (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, and Nanobiotechnology Major, Korea University of Science and Technology) ;
  • Chung, Bong-Hyun (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, and Nanobiotechnology Major, Korea University of Science and Technology) ;
  • Park, Sue-Nie (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration)
  • Published : 2009.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

As nanomaterials might enter into cells and have high reactivity with intracellular structures, it is necessary to assay possible genotoxic risk of them. One of these approaches, we investigated possible genotoxic potential of gold nanoparticle (AuNP) using L5178Y cells. Four different sizes of AuNP (4, 15, 100 or 200 nm) were synthesized and the sizes and structures of AuNP were analyzed using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and stability was analyzed by a UV/Vis. Spectrophotometer. Cytotoxicity was assessed by direct cell counting, and cellular location was detected by dark field microscope at 6, 24 and 48 h after treatment of AuNP. Comet assay was conducted to examine DNA damage and tumor necrosis factor (TNF)-${\alpha}$ mRNA level was assay by real-time reverse transcription polymerase chain reaction. Synthetic AuNP (4, 50, 100 and 200 nm size) had constant characteristics and stability confirmed by TEM, SEM and spectrophotometer for 10 days, respectively. Dark field microscope revealed the location of AuNP in the cytoplasm at 6, 24 and 48 h. Treatment of 4 nm AuNP induced dose and time dependent cytotoxicity, while other sizes of AuNP did not. However, Comet assay represented that treatment of 100 nm and 200 nm AuNP significantly increased DNA damage compared to vehicle control (p <0.01). Treatment of 100 nm and 200 nm AuNP significantly increased TNF-${\alpha}$ mRNA expression compared to vehicle control (p<0.05, p<0.01, respectively). Taken together, AuNP induced DNA damage in L5178Y cell, associated with induction of oxidative stress.

Keywords

References

  1. Balshaw, D. M., Philbert, M. and Suk, W. A. (2005). Research strategies for safety evaluation of nanomaterials, Part III: nanoscale technologies for assessing risk and improving public health. Toxicol. Sci. 88, 298-306 https://doi.org/10.1093/toxsci/kfi312
  2. Chithrani, B. D. and Chan, W. C. (2007). Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes. Nano. Lett. 7, 1542-1550 https://doi.org/10.1021/nl070363y
  3. Chithrani, B. D., Ghazani, A. A. and Chan, W. C. (2006). Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano. Lett. 6, 662-668 https://doi.org/10.1021/nl052396o
  4. Dhawan, A., Taurozzi, J. S., Pandey, A. K., Shan, W., Miller, S. M., Hashsham, S. A. and Tarabara, V. V. (2006). Stable colloidal dispersions of C60 fullerenes in water: evidence for genotoxicity. Environ. Sci. Technol. 40, 7394-7401 https://doi.org/10.1021/es0609708
  5. Dufour, E. K., Kumaravel, T., Nohynek, G. J., Kirkland, D. and Toutain, H. (2006). Clastogenicity, photo-clastogenicity or pseudo-photo-clastogenicity: Genotoxic effects of zinc oxide in the dark, in pre-irradiated or simultaneously irradiated Chinese hamster ovary cells. Mutat. Res. 607, 215-224 https://doi.org/10.1016/j.mrgentox.2006.04.015
  6. El-Sayed, I. H., Huang, X. and El-Sayed, M. A. (2005). Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer. Nano. Lett. 5, 829-834 https://doi.org/10.1021/nl050074e
  7. Frens, G. (1973). Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature Phys. Sci. 241, 20-22 https://doi.org/10.1038/physci241020a0
  8. Gurr, J. R., Wang, A. S., Chen, C. H. and Jan, K. Y. (2005). Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 213, 66-73 https://doi.org/10.1016/j.tox.2005.05.007
  9. Holsapple, M. P. and Lehman-McKeeman, L. D. (2005). Forum series: research strategies for safety evaluation of nanomaterials. Toxicol. Sci. 87, 315 https://doi.org/10.1093/toxsci/kfi286
  10. Jana, N.R., Gearheart, L. and Murphy, C.J. (2001). Wet chemical synthesis of high aspect ratio cylindrical gold nanorods. J. Phys. Chem. B. 105, 4065-4067 https://doi.org/10.1021/jp0107964
  11. Maynard, A. D., Aitken, R. J., Butz, T., Colvin, V., Donaldson, K., Oberdorster, G., Philbert, M. A., Ryan, J., Seaton, A., Stone, V., Tinkle, S. S., Tran, L., Walker, N. J. and Warheit, D. B. (2006). Safe handling of nanotechnology. Nature 444, 267-269 https://doi.org/10.1038/444267a
  12. McNamee, J. P., McLean, J. R., Ferrarotto, C. L. and Bellier, P. V. (2000). Comet assay: rapid processing of multiple samples. Mutat. Res. 466, 63-69 https://doi.org/10.1016/S1383-5718(00)00004-8
  13. Nel, A., Xia, T., Madler, L. and Li, N. (2006). Toxic potential of materials at the nanolevel. Science 311, 622-627 https://doi.org/10.1126/science.1114397
  14. Oberdorster, G., Ferin, J. and Lehnert, B. E. (1994). Correlation between particle size, in vivo particle persistence, and lung injury. Environ. Health Perspect. 102 Suppl 5, 173-179 https://doi.org/10.2307/3432080
  15. Oberdorster, G., Oberdorster, E. and Oberdorster, J. (2005). Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ. Health Perspect. 113, 823-839 https://doi.org/10.1289/ehp.7339
  16. Papageorgiou, I., Brown, C., Schins, R., Singh, S., Newson, R., Davis, S., Fisher, J., Ingham, E. and Case, C. P. (2007). The effect of nano- and micron-sized particles of cobalt-chromium alloy on human fibroblasts in vitro. Biomaterials 28, 2946-2958 https://doi.org/10.1016/j.biomaterials.2007.02.034
  17. Porter, A. E., Gass, M., Muller, K., Skepper, J. N., Midgley, P. A. and Welland, M. (2007). Direct imaging of single-walled carbon nanotubes in cells. Nature Nanotechnology 2, 713-717 https://doi.org/10.1038/nnano.2007.347
  18. Rahman, Q., Lohani, M., Dopp, E., Pemsel, H., Jonas, L., Weiss, D. G. and Schiffmann, D. (2002). Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environ. Health Perspect. 110, 797-800 https://doi.org/10.1289/ehp.02110797
  19. Thomas, K., Aguar, P., Kawasaki, H., Morris, J., Nakanishi, J. and Savage, N. (2006a). Research strategies for safety evaluation of nanomaterials, part VIII: International efforts to develop risk-based safety evaluations for nanomaterials. Toxicol. Sci. 92, 23-32 https://doi.org/10.1093/toxsci/kfj211
  20. Thomas, K. and Sayre, P. (2005). Research strategies for safety evaluation of nanomaterials, Part I: evaluating the human health implications of exposure to nanoscale materials. Toxicol. Sci. 87, 316-321 https://doi.org/10.1093/toxsci/kfi270
  21. Thomas, T., Thomas, K., Sadrieh, N., Savage, N., Adair, P. and Bronaugh, R. (2006b). Research strategies for safety evaluation of nanomaterials, part VII: evaluating consumer exposure to nanoscale materials. Toxicol. Sci. 91, 14-19 https://doi.org/10.1093/toxsci/kfj129

Cited by

  1. The effect of particle size on the genotoxicity of gold nanoparticles vol.105, pp.3, 2017, https://doi.org/10.1002/jbm.a.35944
  2. Acute and chronic administration of gold nanoparticles cause DNA damage in the cerebral cortex of adult rats vol.766-767, 2014, https://doi.org/10.1016/j.mrfmmm.2014.05.009
  3. Evaluation of CdSe/CdS-PEG-FA quantum dots: distribution and observable-adverse-effect-level in mice after intravenous injection vol.42, pp.4, 2012, https://doi.org/10.1007/s40005-012-0026-3
  4. Mechanisms and measurements of nanomaterial-induced oxidative damage to DNA vol.398, pp.2, 2010, https://doi.org/10.1007/s00216-010-3881-7
  5. NIST gold nanoparticle reference materials do not induce oxidative DNA damage vol.7, pp.1, 2013, https://doi.org/10.3109/17435390.2011.626537
  6. Nanoecotoxicity effects of engineered silver and gold nanoparticles in aquatic organisms vol.32, 2012, https://doi.org/10.1016/j.trac.2011.09.007
  7. Scientific Opinion on the re-evaluation of gold (E 175) as a food additive vol.14, pp.1, 2016, https://doi.org/10.2903/j.efsa.2016.4362
  8. Endoplasmic reticulum stress signaling is involved in silver nanoparticles-induced apoptosis vol.44, pp.1, 2012, https://doi.org/10.1016/j.biocel.2011.10.019
  9. Oxidative stress contributes to gold nanoparticle-induced cytotoxicity in human tumor cells vol.24, pp.3, 2014, https://doi.org/10.3109/15376516.2013.869783
  10. The Effects of Polymer Coating of Gold Nanoparticles on Oxidative Stress and DNA Damage vol.39, pp.4, 2009, https://doi.org/10.1177/1091581820927646
  11. Toxicity of gold nanoparticles (AuNPs): A review vol.26, pp.None, 2009, https://doi.org/10.1016/j.bbrep.2021.100991
  12. Chitosan-Coated Gold Nanoparticles Induce Low Cytotoxicity and Low ROS Production in Primary Leucocytes, Independent of Their Proliferative Status vol.13, pp.7, 2021, https://doi.org/10.3390/pharmaceutics13070942