Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of T-cell-Dependent Antibody Production by Quercetin in Mice

  • Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
  • Published : 2009.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

The immunosuppressive properties of flavonoids were examined for the first time by testing their effects on T-cell-mediated antibody production, using a classical plague-forming cell (PFC) assay in mice. Among the tested flavonoids including naringenin, chrysin, flavonol, galangin, quercetin, morin, myricetin and biochanin A, only quercetin, orally administered at 25 mg/kg, significantly inhibited the number of IgMproducing PFCs induced by sheep red blood cells (SRBC). Interestingly, biochanin A (isoflavone) increased the number of PFCs, suggesting an immunostimulatory effect. The other flavonoids tested did not inhibit or enhance PFC response significantly. Quercetin was also found to show thymus atrophy dose-dependently at 5-500 mg/kg. All these results indicate that quercetin inhibits in vivo antibody production probably by inhibiting T-cell function.

Keywords

References

  1. Bullock, R. W. and Moller, E. (1972). Spontaneous B cell activation due to loss of normal mouse serum suppressor. Eur. J. Immunol. 2, 514-517 https://doi.org/10.1002/eji.1830020609
  2. Hirano, T., Oka, K., Kawashima, E. and Akiba, M. (1989). Effects of synthetic and naturally-occurring flavonoids on mitogen-induced proliferation of human peripheral-blood lymphocytes. Life Sci. 45, 1407-1411 https://doi.org/10.1016/0024-3205(89)90028-3
  3. Kim, C. J. and Chung, J. M. (1990). Pharmacological activities of flavonoids (I) Relationships of chemical structures of flavonoids and their inhibitory activity of hypersensitivity. Yakhak Hoiji 34, 348-364
  4. Kim, H. P., Son, K. H., Chang, H. W. and Kang, S. S. (2004). Anti-inflammatory plant flavonoids and cellular action mechanisms. J. Pharmacol. Sci. 96, 229-245 https://doi.org/10.1254/jphs.CRJ04003X
  5. Kimata, M., Inagaki, N. and Nagai, H. (2000). Effects of luteolin and other flavonoids on IgE-mediated allergic reactions. Planta Med. 66, 25-29 https://doi.org/10.1055/s-2000-11107
  6. Middleton, E. and Kandaswami, C. (1993). The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. In The flavonoids: Advances in research since 1986 (J. B. Harborne, Ed.), pp. 619-652, Chapman & Hall, London
  7. Mookerjee, B. K., Lee, T. P., Logue, G. P., Lippes, H. A. and Middleton, E. (1986). The effects of flavonoids on human lymphocyte proliferative responses. In Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological and Structure- activity Relationships (V. Cody et al., Ed.), pp. 511-520, Alan R. Liss, New York
  8. Murota, K. and Terao, J. (2003). Antioxidative flavonoid quercetin: implication of its intestinal absorption and metabolism. Arch. Biochem. Biophys. 417, 12-17 https://doi.org/10.1016/S0003-9861(03)00284-4
  9. Nair, M. P. N., Kandaswami, C., Mahajan, S., Chadha, K. C., Chawda, R., Nair, H., Kumar, N., Nair, R. E. and Schwartz, S. A. (2002). The flavonoid quercetin, differentially regulates Th-1 (IFN-${\gamma}$) and Th-2 (IL-4) cytokine gene expression by normal peripheral blood mononuclear cells. Biochim. Biophys. Acta. 1593, 29-36 https://doi.org/10.1016/S0167-4889(02)00328-2
  10. Namgoong, S. Y., Son, K. H., Chang, H. W., Kang, S. S. and Kim, H. P. (1994). Effects of naturally occurring flavonoids on mitogen-induced lymphocyte proliferation and mixed lymphocyte culture. Life Sci. 54, 313-320 https://doi.org/10.1016/0024-3205(94)00787-X
  11. Ross, J. A. and Kasum, C. M. (2002). Dietary flavonoids: Bioavailability, metabolic effects, and safety. Ann. Rev. Nutr. 22, 19-34 https://doi.org/10.1146/annurev.nutr.22.111401.144957
  12. Schuwartz, A., Sutton, S. L. and Middleton, E. (1982). Quercetin inhibition of the induction and function of cytotoxic T lymphocytes. Immunopharmacol. 4, 125-138 https://doi.org/10.1016/0162-3109(82)90015-7

Cited by

  1. Protective effect of myricetin, apigenin, and hesperidin pretreatments on cyclophosphamide-induced immunosuppression vol.43, pp.3, 2021, https://doi.org/10.1080/08923973.2021.1916525