DOI QR코드

DOI QR Code

식중독 세균에 대한 구아바 부위별 추출물의 항균 특성

Antimicrobial Characteristics of Different Parts of Guava against Food-Borne Bacteria

  • 조영홍 (경남대학교 식품생명학과) ;
  • 옥둘이 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Jo, Young-Hong (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Ok, Dul-lee (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam University)
  • 발행 : 2009.12.31

초록

본 연구는 인공합성보존료를 대체할 수 있는 천연보존료의 개발을 위한 방안으로 대표적인 세균성 식중독 미생물로서 그람 음성균인 Escherichia coli O157:H7과 Salmonella Typhimurium, 그리고 그람 양성균인 Staphylococcus aureus와 Listeria monocytogenes에 대한 항균 특성을 조사하였다. 구아바 가지와 잎의 메탄올과 에탄올 추출물 S. aureus의 생육을 저해하였으며, 아울러 가지와 잎의 아세톤 추출물은 200 ppm 농도의 첨가에서 식중독 원인균 S. aureus균을 전혀 자라지 못하는 결과를 확인하였다. 이러한 결과는 구아바 잎과 가지의 추출물에 그람 양성균에 대한 높은 항균활성이 있음을 의미하며, 구아바 가지를 항균력을 가지는 소재로 이용될 수 있음을 시사한다.

Guava (Psidium guajava L.) contains high amount of vitamins and minerals, and its leaves have been reported to be very effective on reducing blood pressure. In this study, antimicrobial characteristics of extracts from four different parts of guava (fruit, branch, leaf, and seed) with four different solvents (methanol, ethanol, acetone, and water) were evaluated. Four targeted food-borne microorganisms were selected; two Gram negatives (Escherichia coli O157:H7 and Salmonella Typhimurium) and two Gram positives (Staphylococcus aureus and Listeria monocytogenes). By the paper disc method, guava extracts showed stronger clear hollow zone against Gram positive bacteria than Gram negatives in nutrient broth agar plate. Especially, extracts of branches and leaves showed significant antibacterial activity. Guava extracts also showed significant inhibition activity on the growth of Gram positive food-borne bacteria in nutrient broth. For example, S. aureus did not grow at all at 200 ppm of acetone extracts of guava branch and leaf. In the case of L. monocytogenes, the same concentration of acetone extracts of guava branch and leaf inhibited the growth 33.5% and 55.4%, respectively, at 32 hrs of incubation time. The results indicated that extracts of guava branch and leaf showed significant antibacterial activities against food-borne Gram positive microorganisms, and that guava branches, the byproducts of guava, might be a valuable resource for antibacterial materials.

키워드

참고문헌

  1. Greig JD, Pavel A. 2009. Analysis of foodborne outbreak data reported internationally for source attribution. Int J Food Microbiol 130: 77-87 https://doi.org/10.1016/j.ijfoodmicro.2008.12.031
  2. Centers for Disease Control and Prevention. 2009. Surveillance for foodborne disease outbreaks-United States 2006. MMWR Morb Mortal Wkly Rep 58: 609-615
  3. Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH. 2002. Triterpenoids from the leaves of Psidium guajava. Phytochemistry 61: 399-403 https://doi.org/10.1016/S0031-9422(02)00190-5
  4. Fransworth NR, Bunyaprapatsara N. 1990. Thai medicinal plants recommended for primary health care in Thailand. Mahidol University, Bangkok, Thailand. p 202-207
  5. Lozoya X, Reyes-Morales H, Chacez-Soto MA, Martinez- Garcia MC, Soto-Gonzalex Y, Doubova SV. 2002. Intestinal anti-spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease. J Ethenopharmacol 83: 19-24 https://doi.org/10.1016/S0378-8741(02)00185-X
  6. Batick MJ. 1984. Ethnobotany of palms in the neotropics. In Advances in Economic Botany: Ethnobotany in Neotropics. Prance GT, Kallunki JA, eds. New York Botanical Garden, New York, USA. p 9-23
  7. Khan MIH, Ahmad J. 1985. A pharmacognostic study of Psidium guajava L. Int J Crude Drug Res 23: 95-103 https://doi.org/10.3109/13880208509069009
  8. Hong NL. 1998. Chinese Medicinal Herbs of Hong Kong. 2. Hang Chiewing Sa Kwang, Hong Kong. p 104-105
  9. Tanaka T, Ishida N, Ishimatsu M, Nonaka G, Bishioka I. 1992. Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins and psiguavin from the bark of Psidium guajava L. Chem Pharm Bull 40: 2092-2098 https://doi.org/10.1248/cpb.40.2092
  10. Morales MA, Tortoriello J, Meckes M, Paz D, Lozoya X. 1994. Calcium-antagonist effect of quercetin and its relation with spasmolytic properties of Psidium guajava L. Arch Med Res 25: 17-21
  11. Lozoya X, About-Zaid MM, Nozzilillo C, Arnason JT. 1990. Spasmolytic effect of the methanolic extract of Psidium guajava. Planta Medica 56: 686-689
  12. Deguchi Y, Osada K, Uchida K, Kimura H, Yoshika M, Kudo T, Yasui T, Watanuki M. 1998. Effect of extract of guava leaves on the development of diabetes in the db/db mouse and on the postprandial blood glucose of human subjects. Nippon Nogeikagaku Kaishi 71: 923-931
  13. Jin YJ, Kang SH, Choi SY, Park SY, Park JG, Moon SW, Park DB, Kim SJ. 2006. Effect of fermented guava (Psidium guajava L.) leaf extract on hyperglycemia in low dose streptozotocin-induced diabetic mice. Korean J Food Sci Technol 38: 679-683
  14. Yang HJ, Kim EH, Park SN. 2008. Antioxidative activity and component analysis of Psidium guajava leaf extracts. J Soc Cosmet Scientists Korea 34: 233-244
  15. Misra K, Seshadri TR. 1968. Chemical components of the fruits of Psidium guajava. Phytochemistry 7: 641-645 https://doi.org/10.1016/S0031-9422(00)88240-0
  16. Mercadante AZ, Steck A, Pfander H. 1999. Carotenoids from guava (Psidium guajava L.): isolation and structure elucidation. J Agric Food Chem 47: 145-151 https://doi.org/10.1021/jf980405r
  17. Miean KH, Mohamed S. 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem 49: 3106-3112 https://doi.org/10.1021/jf000892m
  18. Chen JT, Yang RS. 1983. Hypoglycemic effect of guava juice in mice and human subjects. Am J Chin Med 11: 74-76 https://doi.org/10.1142/S0192415X83000124
  19. Oljide OA, Awe SO, Makinde JM. 1999. Pharmacological studies on the leaf of Psidium guajava. Fitoterapia 70: 25-31 https://doi.org/10.1016/S0367-326X(98)00010-0
  20. Arima H, Danno G. 2002. Isolation of antimicrobial compounds from guava (Psidium guajava L.) and their structural elucidation. Biosci Biotechnol Biochem 66: 1727-1730 https://doi.org/10.1271/bbb.66.1727
  21. Ojan H, Nihorimbere V. 2004. Antioxidant power of phytochemicals from Psidium guajava. J Zhejiang Univ Sci 5: 676-683 https://doi.org/10.1631/jzus.2004.0676
  22. Yuk HG, Jo SC, Seo HK, Park SM, Lee SC. 2008. Effect of storage in juice with or without pulp and/or calcium lactate on the subsequent survival of Escherichia coli O157:H7 in simulated gastric fluid. Int J Food Microbiol 123: 198-203 https://doi.org/10.1016/j.ijfoodmicro.2008.01.013
  23. Kim HJ, Lee SC. 2002. Antimicrobial activity of silver ion against Salmonella typhimuruim, Staphylococcus aureus and Vibrio parahaemolyticus. J Korean Soc Food Sci Nutr 31: 1163-1166 https://doi.org/10.3746/jkfn.2002.31.6.1163
  24. Pelegrini PB, Murad AM, Silva LP, dos Santos RCP, Costa FT, Tagliari PD, Bloch Jr C, Noronha EF, Miller RNG, Franco OL. 2008. Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides 29: 1271-1279 https://doi.org/10.1016/j.peptides.2008.03.013
  25. Mahfuzul HMD, Bari ML, Inatsu Y, Juneja VK, Kawamoto S. 2007. Antibacterial activity of guava (Psidium guajava L.) and Neem (Azadirachta indica A. Juss.) extracts against foodborne pathogens and spoilage bacteria. Foodborne Pathog Dis 4: 481-488 https://doi.org/10.1089/fpd.2007.0040
  26. Voravuthikunchai S, Lortheeranuwat A, Jeeju W, Sririrak T, Phongpaichit S, Supawita T. 2004. Effective medicinal plants against enterohaemorrhagic Escherichia coli O157:H7. J Ethnopharmacol 94: 49-54 https://doi.org/10.1016/j.jep.2004.03.036
  27. Goncalves FA, Andrade NM, Bezerra JN, Macrae A, Sousa OV, Fonteles-Filho AA, Vieira RH. 2008. Antibacterial activity of guava, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp, Xiphopenaeus kroyeri (Heller). Rev Inst Med Trop Sao Paulo 50: 11-15 https://doi.org/10.1590/S0036-46652008000100003
  28. Gnam SO, Demello MT. 1999. Inhibition of Staphylococcus aureus by aqueous Goiaba extracts. J Ethnopharmacol 68: 103-108 https://doi.org/10.1016/S0378-8741(99)00048-3
  29. Prabu GR, Gnanamani A, Sadulla S. 2006. Guaijaverin a plant flavonoid as potential antiplaque agent against Streptococcus mutans. J Appl Microbiol 101: 487-495 https://doi.org/10.1111/j.1365-2672.2006.02912.x

피인용 문헌

  1. Effect of Aqueous Extract of Guava (Psidium guajava L.) Leaves on the Oral Glucose Tolerance Test and Inhibition of Glycoside Hydrolase vol.20, pp.2, 2012, https://doi.org/10.7783/KJMCS.2012.20.2.094
  2. Antimicrobial Activity of Myagropsis yendoi Extract vol.43, pp.6, 2010, https://doi.org/10.5657/kfas.2010.43.6.642
  3. Natural Dyeing of Fabrics with Guava (Psidium guajava L.) Leaf Extract II - Dyeability and Functional Property of Cotton Fabrics - vol.14, pp.2, 2012, https://doi.org/10.5805/KSCI.2012.14.2.320
  4. Neuronal Cell Protective Effects of Hot Water Extracts from Guava (Psidium guajava L.) Fruit and Leaf vol.18, pp.1, 2011, https://doi.org/10.11002/kjfp.2011.18.1.124
  5. Antioxidant Activity and Antimicrobial Effect for Foodborne Pathogens from Extract and Fractions of Sanguisorba officinalis L. vol.24, pp.4, 2016, https://doi.org/10.7783/KJMCS.2016.24.4.303
  6. Exposure of Salmonella Typhimurium to guava extracts increases their sensitivity to acidic environments vol.33, pp.2, 2013, https://doi.org/10.1016/j.foodcont.2013.03.033
  7. Natural Dyeing of Fabrics with Guava (Psidium guajava L.) Leaf Extract III - Dyeability and Functional Property of Hanji Cotton Fabrics - vol.14, pp.5, 2012, https://doi.org/10.5805/KSCI.2012.14.5.866
  8. Effect of guava extracts on heat resistance of Salmonella Typhimurium vol.22, pp.6, 2013, https://doi.org/10.1007/s10068-013-0280-9
  9. 구아바 잎 추출물의 항산화 및 미백 활성 효과 vol.34, pp.2, 2017, https://doi.org/10.12925/jkocs.2017.34.2.296
  10. 구아바 잎 추출물의 항산화 및 미백 활성 효과 vol.34, pp.2, 2017, https://doi.org/10.12925/jkocs.2017.34.2.296