Journal of Plant Biotechnology

  • 제36권4호
  • /
  • Pages.403-406
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  • 2009
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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한지형 마늘에 있어서 기내뿌리로부터 식물체 재분화

Plant regeneration from callus derived root of northen type in garlic (Allium sativum L.)

  • 안율균 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 김도선 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 윤무경 (농촌진흥청 국립원예특작과학원 채소과)
  • Ahn, Yul-Kyun (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Do-Sun (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Moon-Kyoung (Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA)
  • 발행 : 2009.12.31
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초록

This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both ‘Danyang' and ‘Euseong'. The frequency of callus formation were 81.2% ‘Danyang' and 76.1% ‘Euseong'. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for ‘Danyang' and MS supplemented with 10 mg/L BAP for ‘Euseong'. The frequency of shoot regeneration were 51.5% ‘Danyang' and 56.6% ‘Euseong' The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.

키워드

참고문헌

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피인용 문헌

  1. Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.) vol.41, pp.3, 2014, https://doi.org/10.5010/JPB.2014.41.3.140