The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Variation of Scytalone Dehydratase Gene in Colletotrichum spp.

Colletotrichum spp. 유래 scytalone dehydratase 유전자의 유전적 다양성 비교

  • Yun, Yeo-Hong (Department of Microbiology, Dankook University) ;
  • Hyeon, Min-U (Department of Microbiology, Dankook University) ;
  • Suh, Dong-Yeon (Department of Microbiology, Dankook University) ;
  • Kim, Yong-Min (Department of Microbiology, Dankook University) ;
  • Kim, Seong-Hwan (Department of Microbiology, Dankook University,Institute of Basic Sciences, Dankook University) ;
  • Choi, Chang-Won (Department of Biology and Medicinal Science, Paichai University) ;
  • Kim, Young-Shick (Department of Plant Science and Technology, Sangmyung University)
  • 윤여홍 (단국대학교 미생물학과) ;
  • 현민우 (단국대학교 미생물학과) ;
  • 서동연 (단국대학교 미생물학과) ;
  • 김용민 (단국대학교 미생물학과) ;
  • 김성환 (단국대학교 미생물학과,기초과학연구소) ;
  • 최창원 (배재대학교 생물의약학과) ;
  • 김영식 (상명대학교 식물산업공학과)
  • Published : 2009.12.31
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Abstract

The production of dihydroxynaphthalene (DHN) melanin is known to be essential factor for pathogenicity in Colletotrichum lagenarium. However, the genetic diversity of melanin genes was not much known among Colletotrichum spp. To investigate the variability of melanin gene in Colletotrichum spp. that cause anthracnose on diverse crops including tomato, we cloned and sequenced partial sd, one of DHN melanin genes encoding for scytalone dehydratase, from eight strains of C. coccodes, C. acutatum, C. truncatum C. caricae, and C. musae. The size of PCR-amplified sd ranged 437 bp to 545 bp. The nucleotide sequence identity of sd among the Colletotrichum strains tested varied from 49% to 99%. All of the PCR-amplified sd from eight strains contain an intron and have two exons coding for 122 amino acids. Overall, the size and nucleotide sequence of sd varied among the five Colletotrichum spp. Sequence identity of the predicted scytalone dehydratase protein of 122 amino acids ranged 50 to 99%. Phylogentic analysis based on the sd nucleotide sequences revealed that the five Colletotrichum spp. could be genetically divided.

Keywords

References

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