DOI QR코드

DOI QR Code

Efficient Colorimetric Assay of RNA Polymerase Activity Using Inorganic Pyrophosphatase and Ammonium Molybdate

  • Lee, Bok-Hui (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Park, Sun-Young (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Heo, Yong-Seok (Department of chemistry, Konkuk University) ;
  • Yea, Sung-Su (Department of Biochemistry, College of Medicine, Inje University) ;
  • Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
  • Published : 2009.10.20

Abstract

Keywords

References

  1. Chen, J.; Brevet, A.; Fromant, M.; Leveque, F.; Schmitter, J. M.; Blanquet, S.; Plateau, P. J. Bacteriol. 1990, 172, 5686.
  2. Lundin, M.; Baltscheffsky, H.; Ronne, H. J. Biol. Chem. 1991, 266, 12168.
  3. Kornberg, A. On the Metabolic Significance of Phosphorolytic and Pyrophosphorolytic Reactions; Academic Press: New York, 1962.
  4. Cogan, E. B.; Birrell, G. B.; Griffith, O. H. Anal. Biochem. 1999, 271, 29. https://doi.org/10.1006/abio.1999.4100
  5. Black, M. J.; Jones, M. E. Anal. Biochem. 1983, 135, 233. https://doi.org/10.1016/0003-2697(83)90756-X
  6. Fiske, C. H.; Subbarow, Y. J. Biol. Chem. 1925, 66, 375.
  7. Lowry, O. H.; Lopez, J. A. J. Biol. Chem. 1946, 162, 421.
  8. Lanzetta, P. A.; Alvarez, L. J.; Reinach, P. S.; Candia, O. A. Anal. Biochem. 1979, 100, 95. https://doi.org/10.1016/0003-2697(79)90115-5
  9. Taussky, H. H.; Shorr, E. J. Biol. Chem. 1953, 202, 675.
  10. Gawronski, J. D.; Benson, D. R. Anal. Biochem. 2004, 327, 114. https://doi.org/10.1016/j.ab.2003.12.024
  11. Mahuren, J. D.; Coburn, S. P.; Slominski, A.; Wortsman, J. Anal. Biochem. 2001, 298, 241. https://doi.org/10.1006/abio.2001.5402
  12. Harder, K. W.; Owen, P.; Wong, L. K.; Aebersold, R.; Clark- Lewis, I.; Jirik, F. R. Biochem. J. 1994, 298(Pt 2), 395.
  13. Gibson, N. J.; Newton, C. R.; Little, S. Anal. Biochem. 1997, 254, 18. https://doi.org/10.1006/abio.1997.2324
  14. Vassiliou, W.; Epp, J. B.; Wang, B. B.; Del Vecchio, A. M.; Widlanski, T.; Kao, C. C. Virology 2000, 274, 429. https://doi.org/10.1006/viro.2000.0492
  15. Lahser, F. C.; Malcolm, B. A. Anal. Biochem. 2004, 325, 247. https://doi.org/10.1016/j.ab.2003.10.034
  16. Jia, Y.; Patel, S. S. J. Biol. Chem. 1997, 272, 30147. https://doi.org/10.1074/jbc.272.48.30147
  17. Bandwar, R. P.; Patel, S. S. J. Biol. Chem. 2001, 276, 14075.

Cited by

  1. A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe vol.49, pp.80, 2013, https://doi.org/10.1039/c3cc45750h
  2. The effects of light and temperature on microalgal growth and nutrient removal: an experimental and mathematical approach vol.6, pp.27, 2016, https://doi.org/10.1039/C5RA26117A
  3. Identification of a bifunctional primase-polymerase domain of corynephage BFK20 replication protein gp43 vol.163, pp.2, 2009, https://doi.org/10.1016/j.virusres.2011.11.005
  4. Processing Methodologies of Wet Microalga Biomass Toward Oil Separation: An Overview vol.26, pp.3, 2009, https://doi.org/10.3390/molecules26030641