Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Identification of Korean Strawberry Cultivars using DNA markers

DNA 표지를 이용한 딸기 국내 육성 품종 판별

  • Cho, Kang-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Rho, Il Rae (National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Yong Seop (National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Pue-Hee (National Institute of Horticultural & Herbal Science, RDA)
  • Received : 2008.12.15
  • Published : 20081200
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Abstract

This study was conducted to develop the DNA markers for identification of the strawberry cultivars in Korea and Japan. We developed fifteen cleaved amplified polymorphic sequence (CAPS) markers based on the Fragaria gene sequences. Among them six CAPS markers showed polymorphism exclusively in one cultivar. Five CAPS markers (ANR-MspI, ANR-BamHI, ACO-HinfI, DFR-AseI, FGT-MspI) provided enough polymorphism to identify eight Korean strawberry cultivars except for 'Maehyang' and 'Sunhong'. To complement the fifteen CAPS markers, we selected another fifteen sequence-related amplified polymorphism (SRAP) and one of them, me1/em5_460bp marker, made it possible to discriminate between 'Maehyang' and 'Sunhong'. Therefore, application of the five CAPS markers and one SRAP marker were sufficient to identify the nineteen Korean and Japanese strawberry cultivars. These markers could be used practically for cultivar identification of Korean and Japanese strawberry.

딸기 국내 육성 신품종을 정확히 판별할 수 있는 DNA표지를 개발하고자 실험을 수행하였다. 딸기 유전정보를 이용하여 품종 판별이 가능한 CAPS 표지 15종을 개발하였고, 그 중에서 6종은 품종 특이적인 표지였다. CAPS 표지 중에서 ANR-MspI, ANR-BamHI, ACO-HinfI, DFR-AseI, FGT-MspI의 최소 5종의 표지를 이용하여 '매향'과 '선홍'을 제외한 국내 육성 품종 판별이 가능하였다. 15종의 CAPS 표지를 보완하기 위해 SRAP 분석을 통해 품종 간 다형성을 나타내는 15종의 표지를 선발하였고, 그 중에서 me1/em5-460bp 표지를 이용하여 '매향'과 '선홍'의 구별이 가능하였다. 따라서 5종의 CAPS 표지와 1종의 SRAP 표지를 이용하여 19종의 국내 육종 품종과 일본 품종의 판별이 가능하였으며, 금후 이 연구결과는 딸기 국내 육성 품종 식별을 위해 효과적으로 이용될 수 있을 것으로 판단되었다.

Keywords

Acknowledgement

Grant : 딸기 국내 육성 품종 판별표지 및 탄저병 저항성 DNA 표지 개발

Supported by : 농촌진흥청

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