Measurement of Leukocyte Motions in a Microvessel Using Spatiotemporal Image Analysis

  • Kim, Jin-Woo (Department of Multimedia Communication Engineering, Kyungsung University)
  • Published : 2008.09.30

Abstract

This paper describes a method for recognizing and measuring the motion of each individual leukocyte in microvessel from a sequence of images. A spatiotemporal image is generated whose spatial axes are parallel and vertical to vessel region contours. In order to enhance and extract only leukocyte traces with a turned velocity range even under noisy background, we use a combination of a filtering process using Gabor filters with sharp orientation selectivity and a subsequent 3D spatiotemporal grouping process. The proposed method is shown to be effective by experiments using image sequences of two kinds of microcirculation, rat mesentery microvessels and human retinal capillaries.

Keywords

References

  1. G.W.Schmid-Schonbein, S.Usami, R.Skalak, and S.Chien, 'The interaction of leukocytes and erythrocytes in capillary and postcapillary vessels', Microvas. Res., vol. 19, pp. 45-70, 1980 https://doi.org/10.1016/0026-2862(80)90083-7
  2. H.Komatsu, A.Koo, and P.H.Guth, 'Leukocyte flow dynamics in the rat liver microcirculation', Microvas. Res., vol. 40, pp. 1-13, 1990 https://doi.org/10.1016/0026-2862(90)90002-9
  3. U.H.von Andrian, J.D.Chambers, L.M.Mcevoy, R.F.Bargaze, K.E.Arfors, and E.C.Butcher, 'Two-step model of leukocyte-endothelial cell interaction in inammation: distinct roles for lecam-1 and the leukocyte 2 integrins in vivo', Proc. Natl. Acad. Sci. USA New York, vol. 88, pp. 7538-7542, 1991
  4. S.Wolf. O.Arend, H.Toonen, B.Bertram, F.Jung, and M.Reim, 'Retinal capillary blood flow measurement with a scanning laser ophthalmoscope,' Ophthalmology, vol. 98, no. 6, pp.996-1000, 1991 https://doi.org/10.1016/S0161-6420(91)32192-4
  5. T.Tanaka, K.Muraoka, and K.Shimizu, 'Fluorescein fundus angiography with scanning laser ophthalmoscope,' Ophthalmology, vol. 98, no. 12, pp.1824-1829, 1991 https://doi.org/10.1016/S0161-6420(91)32043-8
  6. B.P.Fleming, B.Klitzman, and W.O.Johnson, 'Measurement of erythrocyte velocity by use of a periodic differential detector,' American Journal of Physiology, vol. 249, (Heart Circ. Pysiol. 18)H899-H905, 1985
  7. N.Tateishi, M.Okazaki, and T.Shiga, 'Determination and kinetics of platelet adhesion onto material surfaces,' Journal of Membrane Science, vol.41, pp.315-322, 1989 https://doi.org/10.1016/S0376-7388(00)82411-4
  8. C.Y.J.Yip, S.Y.Aggarwal, K.R.Diller, and S.C.Bovik, 'Simultaneous multiple site arteriolar vasomotion measurement using digital image analysis,' Microvaslular Research, vol.41, pp. 73-83, 1991 https://doi.org/10.1016/0026-2862(91)90009-Z
  9. R.C.Bolles and H.H.Barker, 'Epipolar plane image analysis: A technique for analyzing motion sequences,' Proc. 3rd Int. Symposium on Robotics Research, pp. 41-48, 1986
  10. D.J.Heeger, 'Optical flow from spatiotemporal filters,' Proc. 1st ICCV, pp.181-190, 1987
  11. S.C.Bovik, M.Clark, and W.S.Geisler, 'Multichannel texture analysis using localized spatial filters,' IEEE Trans. On PAMI, vol.12, no.1, pp.55-73,1990 https://doi.org/10.1109/34.41384