Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Cellular Imaging of Gold Nanoparticles Using a Compact Soft X-Ray Microscope

연 X-선 현미경을 이용한 금 나노입자 세포영상

  • Kwon, Young-Man (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Kim, Han-Kyong (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Kim, Kyong-Woo (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Kim, Sun-Hee (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Yin, Hong-Hua (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Chon, Kwon-Su (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Kang, Sung-Hoon (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Park, Seong-Hoon (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Juhng, Seon-Kwan (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine) ;
  • Yoon, Kwon-Ha (Department of Radiology and Institute for Radiological Imaging Science, Wonkwang University, School of Medcine)
  • 권영만 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 김한경 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 김경우 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 김선희 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 윤홍화 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 천권수 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 강성훈 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 박성훈 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 정선관 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소) ;
  • 윤권하 (원광대학교 의과대학 영상의학과 및 익산방사선영상과학연구소)
  • Published : 2008.09.30
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Abstract

A compact soft x-ray microscope operated in the 'water window' wavelength region ($2.3{\sim}4.4nm$) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

연 x-선 현미경은 '물의 창' 영역 ($2.3{\sim}4.4nm$)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다.

Keywords

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