The Transactions of The Korean Institute of Electrical Engineers (전기학회논문지)

The Korean Institute of Electrical Engineers (대한전기학회)
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Electrochemical Detection of Hydrogen Peroxide based on Viologen Monolayers

Viologen 박막을 이용한 과산화수소의 전기화학적 검출 특성

  • Published : 2008.11.01
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Abstract

In this paper, we fabricated a biosensor for detecting hydrogen peroxide and investigated the sensing property. We prepared a viologen and hemoglobin modified gold electrode using self-assembly and layer by layer method. The electrochemical property of the viologen derivative was characterized in 0.1 M $NaClO_4$ electrolyte solution by cyclic voltammetry. The modified electrode showed reversible electrochemical properties and high stability. From the results, the viologen can act as a charge transfer mediator for access to the electrode surface. The catalytic characteristics of the designed sensor proved that hemoglobin has been kept in its natural structure and can retain its biological activity. The designed biosensor showed a fast amperometric response, excellent linearity and low detection limit. In addition, it had high sensitivity, good reproducibility and stability.

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References

  1. A. Morrin, A Guzman, A. J. Killard, J. M. Pingarron, M. R. Smyth, 'Characterisation of horseradish peroxidase immobilisation on an electrochemical biosensor by colorimetric and amperometric techniques', Biosens. Bioelecton. 18, 75, 2003
  2. Z. Tong, R. Yuan, Y. Chai, S. Chen, Y. Xie, 'Amperometric biosensor for hydrogen peroxide based on Hemoglobin/DNA/Poly-2,6-pyridinedamine modified gold electrode', Thin Solid Films, 515, 8054, 2007
  3. A.K.M. Kafi, F. Yin, H.-K. Shin, Y.-S. Kwon, 'Hydrogen peroxide biosensor based on DNA - Hb modified gold electrode', Thin Solid Films 499, 420, 2006
  4. C. Nistor, J. Emneus, L. Gorton, A. Ciusu, 'Improved stability and altered selectivity of tyrosinase based graphite electrodes for detection of phenolic compounds', Anal. Chim. Acta, 387, 309, 1999
  5. G. F. Hall, D. J. Best, A. P. F. Turner, 'The determination of p-cresol in chloroform with an enzyme electrode used in the organic phase', Anal Chim. Acta, 213, 13, 1988
  6. D. A. Buttry, M. D. Ward, 'Measurement of interfacial processes at electrode surfaces with the electrochemical quartz crystal microbalance', Chem. Rev., 92, 1355 (1992)
  7. D. Y. Lee, A.K.M. Kafi, S. H. Park, D. J. Qian, Y. S. Kwon, 'Influence of Anions on Electrochemical Redox Reactions and Electrical Properties using a Viologen Derivative', Jpn. J. Appl Phys., 45(4B), 3772, 2006
  8. G. Sauerbrey, 'The uses of a Quartz Crystal Vibrator from Weighting Thin Films on a Microbalance', Z. Phy., 155, 206 (1959)
  9. D. Y. Lee, A.K.M. Kafi, S. H. Park, Y. S. Kwon, 'Charge Transfer Property of Self-Assembled Viologen Derivative by Electrochemical Quartz Crystal Microbalance Response', J. Nanosci. Nanotehnol, 6, 3657, 2006
  10. H. N. Choi, M. A. Kim, W.-Y. Lee, 'Amperometric glucose biosensor based on sol-gel-derived metal oxide/Nafion composite films', Analytica Chimica Acta, 537, 179, 2005
  11. A.K.M. Kafi, D.-Y. Lee, S.-H. Park, Y.-S. Kwon, 'Development of a peroxide biosensor made of a thiolated-viologen and hemoglobin-modified gold electrode', Microchemical J., 8, 308, 2007
  12. L. Shang, Z. Sun, X. Wang, G. Li, 'Enhanced Peroxidase Activity of Hemoglobin in a DNA Membrane and Its Application to an Unmediated Hydrogen Peroxide Biosensor', Anal. Sci. 19 (2003) 1537 https://doi.org/10.2116/analsci.19.1537