한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

옻추출물 처리에 의한 U937 세포에서의 특정 RNA 발현 양상

Isolation and Elucidation of Specific RNAs by Treatment of Rhus verniciflua Stokes Extract to U937 Cell

  • 정미영 (고려대학교 생명공학원) ;
  • 오세욱 (한국식품연구원 안전성연구단)
  • Jeong, Mi-Young (School of Life Sciences and Biotechnology, Korea University) ;
  • Oh, Se-Wook (Food Safety Research Division, Korea Food Research Institute)
  • 발행 : 2008.10.31
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초록

사람의 혈액 내의 단핵구 U937을 모델시스템으로 이용하여 옻추출물 처리에 의해 발현이 조절되는 특정 유전자를 탐색하였다. DDRT-PCR을 이용하여 옻 추출물 처리 시 발현이 감소되는 클론을 하나 얻을 수 있었으며 이를 클로닝하고 염기서열 분석을 실시하였다. 그 결과 얻어진 유전자는 H2A histone family의 member Z와 100% 유사성이 있는 것으로 나타났다. 이 단백질은 특정 조건 하에서 특정한 유전자 발현을 증가시키는 역할을 하는 것으로 보고되고 있으나, 보다 정확한 작용기작을 알아내기 위해서는 유전자 관계 파악을 위하여 향후 계속적인 연구가 필요함을 알 수 있었다.

Differential display RT-PCR was used for screening the differentially expressed specific genes by Rhus verniciflua extract treatment to U937 cell, human leukemic monocyte. As a result, 19 clones differentially expressed were detected. Among the detected clones, one clone was confirmed to be over-expressed by R. verniciflua extract treatment in Northern blot analysis. Nucleotide sequence of the clone showed 100% homology with H2A histone family member Z gene. Therefore, it is concluded that the treatment of R. verniciflua extract to U937 cell specifically induces the expression of H2A.Z gene but its role should be elucidated by future works.

키워드

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