옻추출물 처리에 의한 U937 세포에서의 특정 RNA 발현 양상

Isolation and Elucidation of Specific RNAs by Treatment of Rhus verniciflua Stokes Extract to U937 Cell

  • 정미영 (고려대학교 생명공학원) ;
  • 오세욱 (한국식품연구원 안전성연구단)
  • Jeong, Mi-Young (School of Life Sciences and Biotechnology, Korea University) ;
  • Oh, Se-Wook (Food Safety Research Division, Korea Food Research Institute)
  • 발행 : 2008.10.31

초록

사람의 혈액 내의 단핵구 U937을 모델시스템으로 이용하여 옻추출물 처리에 의해 발현이 조절되는 특정 유전자를 탐색하였다. DDRT-PCR을 이용하여 옻 추출물 처리 시 발현이 감소되는 클론을 하나 얻을 수 있었으며 이를 클로닝하고 염기서열 분석을 실시하였다. 그 결과 얻어진 유전자는 H2A histone family의 member Z와 100% 유사성이 있는 것으로 나타났다. 이 단백질은 특정 조건 하에서 특정한 유전자 발현을 증가시키는 역할을 하는 것으로 보고되고 있으나, 보다 정확한 작용기작을 알아내기 위해서는 유전자 관계 파악을 위하여 향후 계속적인 연구가 필요함을 알 수 있었다.

Differential display RT-PCR was used for screening the differentially expressed specific genes by Rhus verniciflua extract treatment to U937 cell, human leukemic monocyte. As a result, 19 clones differentially expressed were detected. Among the detected clones, one clone was confirmed to be over-expressed by R. verniciflua extract treatment in Northern blot analysis. Nucleotide sequence of the clone showed 100% homology with H2A histone family member Z gene. Therefore, it is concluded that the treatment of R. verniciflua extract to U937 cell specifically induces the expression of H2A.Z gene but its role should be elucidated by future works.

키워드

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