Development of a Genistein-enriched Doenjang Using Corn $\beta$-Glucosidase

  • Oh, Jee-Hwan (Department of Foods and Nutrition, Kookmin University) ;
  • Suh, Joo-Won (Division of Biological Science and Bioinformatics, Myongji University) ;
  • Kim, Jin-Yong (Division of Biological Science and Bioinformatics, Myongji University) ;
  • Lee, In-Hyung (Department of Foods and Nutrition, Kookmin University)
  • 발행 : 2008.10.31

초록

Genistein, one of the isoflavones in doenjang, is generally known to prevent various cancers, osteoporosis, climacterium, and menopause symptoms, and has better bioavailability and healthful physiological effects than its glucoside, genistin. In both traditional and commercial doenjangs, genistein content ranged from 370 to 1,510 mg/kg, however, significant amounts of genistin also existed at the level of 190 to 350 mg/kg. After treating with corn $\beta$-glucosidase, over 84% of genistin in doenjang was converted to genistein. However, physiochemical characteristics such as pH, viscosity, 2-thiobarbituric acid (TBA) value, and color were not changed significantly after corn $\beta$-glucosidase treatments. Therefore, this study shows that the improved doenjang with the increased genistein content can be produced using corn $\beta$-glucosidase.

키워드

참고문헌

  1. Choi SY, Cheigh MJ, Lee JJ, Kim HJ, Hong SS, Chung KS, Lee BK. Growth suppression effect of traditional fermented soybean paste (doenjang) on the various tumor cells. J. Korean Soc. Food Sci. Nutr. 28: 458-463 (1999)
  2. USDA, Iowa State University. Database on the isoflavone contents of foods. Available from: http://www.nal.usda.gov/fnic/foodcomp/Data/isoflav/isoflav.html. Accessed Oct. 25, 2004
  3. Brown JP. Hydrolysis of glycosides and esters. pp. 109-144. In: Role of the Gut Flora Intoxicity and Cancer. Rowland IR (ed). Academic Press, San Diego, CA, USA (1988)
  4. Bae EA, Kwon TW, Moon GS. Isoflavone contents and antioxidative effects of soybeans, soybean curd, and their byproducts. J. Korean Soc. Food Sci. Nutr. 26: 371-375 (1997)
  5. Krishinan HB. Identification of genistein, an anticarcinogenic compound, in the edible tubers of the American groundnut (Apios americana Medikus). Crop Sci. 38: 1052-1056 (1998) https://doi.org/10.2135/cropsci1998.0011183X003800040028x
  6. Strauss L, Mäkela S, Joshi S, Huhtaniemi I, Santti R. Genistein exerts estrogenlike effects in male mouse reproductive tract. Mol. Cell Endocrinol. 144: 83-93 (1998) https://doi.org/10.1016/S0303-7207(98)00152-X
  7. Wang HJ, Murphy PA. Isoflavone contents in commercial soybean foods. J. Agr. Food. Chem. 42: 1666-1673 (1994) https://doi.org/10.1021/jf00044a016
  8. Wang HJ, Murphy PA. Isoflavone composition of American and Japanese soybeans in Iowa: Effects of variety, crop year, and location. J. Agr. Food Chem. 42: 1674-1677 (1994) https://doi.org/10.1021/jf00044a017
  9. Jeong JH, Kim JS. Studies on the contents of free amino acids, organic acids, and isoflavones in commercial soybean paste. J. Korean Soc. Food Sci. Nutr. 27: 10-15 (1998)
  10. Oh JH, Lee I. Search for ${\beta}-glucosidase$ to convert glycons to aglycons in foods. J. Sci. Inst. Kookmin Univ., Korea 26: 161-167 (2008)
  11. Zuccarello F, Buemi G, Gandolfo C, Contino A. Barbituric and thiobarbituric acids: A conformational and spectroscopic study. Spectrochim. Acta A 59: 139-151 (2003) https://doi.org/10.1016/S1386-1425(02)00146-4
  12. Lee MH, Park YH, Oh HS, Kwak TS. Isoflavone content in soybean and its processed products. J. Food Sci. Technol. 34: 365-369 (2002)
  13. Kim JS, Yoon S. Isoflavone contents and ${\beta}-glucosidase$ activity of soybean, meju, and doenjang. Korean J. Food Nutr. 31: 1405-1409 (1999)
  14. Lori C, Neil CB, Kenneth D, Setchell R, Stephen B. Genistein, daidzein, and their ${\beta}-glucoside$ conjugates: Antitumor isoflavones in soybeans foods from American and Asian diets. J. Agr. Food Chem. 41: 1961-1967 (1993) https://doi.org/10.1021/jf00035a027
  15. Friend DR, Chang GW. A colon-specific drug delivery system based on drug glycosides and the glycosidases of colonic bacteria. J. Med. Chem. 27: 261-266 (1984) https://doi.org/10.1021/jm00369a005
  16. Park YK, Alencar SM, Nery IA, Aguiar CL, Pacheco TARC. Enrichment of isoflavone aglycones in extracted soybean isoflavones by heat and fungal ${\beta}-glucosidase$. Food Sci. Ind. 34: 14-19 (2001)
  17. Seo JS, Han EM, Lee TS. Effect of meju shapes and strains on the chemical composition of soybean paste. J. Korean Soc. Food Sci. Nutr. 4: 1-9 (1986)
  18. Miura M, Gomyo T. Effects of melanoidin on cholesterol in plasma, liver, and feces in rats fed a high-cholesterol diet. Agr. Biol. Chem. 52: 2403-2408 (1988) https://doi.org/10.1271/bbb1961.52.2403
  19. Vendenbergh PA. Lactic acid bacteria, their metabolic products and interference with microbial growth. FEMS Microbiol. Rev. 12: 221-237 (1993) https://doi.org/10.1111/j.1574-6976.1993.tb00020.x
  20. Lee YS, Homma S. Desalting by electrodialysis to measure iron chelating activity of melanoidin in soy sauce and fish sauce. Nippon Shokuhin Kogyo Gakk. 38: 556-562 (1991) https://doi.org/10.3136/nskkk1962.38.556