Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anticancer Activity of Ethanol Extract from Peel of Citrus junos and Poncirus trifoliata on MCF-7 Breast Cancer Cells

유자와 탱자 과피 에탄올 추출물의 MCF-7 유방암 세포에 대한 항암 활성

  • Kim, Ji-Eun (Department of Biotechnology, Dong-A University) ;
  • Park, Joon-Hee (Department of Molecular Biology, Dong-Eui University) ;
  • Kang, Byoung-Won (BK21 Center for Silver-Bio industrialization Project, Dong-A University) ;
  • Seo, Min-Jeong (Department of Biotechnology, Dong-A University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Lim, Hak-Seob (Department Bioinstitute, MILLENNIUM PROMISE CO., LTD.) ;
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University) ;
  • Kim, Jeong-In (School of Food and Life Science, Inje University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University)
  • 김지은 (동아대학교 생명공학과) ;
  • 박준희 (동의대학교 분자생물학과) ;
  • 강병원 (동아대학교 BK21 실버바이오 사업단) ;
  • 서민정 (동아대학교 생명공학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 임학섭 ((주)천년약속 바이오연구소) ;
  • 서권일 (순천대학교 식품영양학과) ;
  • 김정인 (인제대학교 식품생명과학부) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 이복규 (동의대학교 분자생물학과) ;
  • 정영기 (동아대학교 생명공학과)
  • Published : 2008.10.30
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Abstract

In this study, anti-cancer activities of peel of Citrus junos (CJP) and Poncirus trifoliata (PTP) on MCF-7 breast cancer cells, and anti-proliferative effects of cancer cells induced by environmental hormones were investigated. The ethanol extracts of CJP and PTP inhibited cancer cell growth and induced apoptosis at the concentration over 300 mg/ml treatment for 72 hr. Morphological change of MCF-7 breast cancer cells were observed treated with the CJP and PTP of 500 mg/ml concentration for 72 hr, and apoptosis was induced by activation of caspase-3. The proliferation of MCF-7 breast cancer cells was decreased in a dose-dependent manner treated with various concentration of CJP and PTP, when compared with the control at 300 mg/ml, the proliferation of the MCF-7 breast cancer cells of both extracts was decreased over 70% and 80%, respectively.

본 연구는 MCF-7 유방암 세포에 대한 유자(CJP)와 탱자 (PTP) 과피 추출물의 항암 활성과 환경호르몬에 의해 유도된 암세포의 증식 억제 효과에 대하여 조사하였다. CJP와 PTP를 300 mg/ml 농도에서 72시간 처리하였을 경우, 암세포의 성장을 저해하였고 세포사멸을 유도하였다. MCF-7 유방암 세포의 형태학적 변화는 CJP와 PTP를 500 mg/ml 농도에서 7시간 처리하였을 경우 관측되었고 세포사멸은 capase-3의 활성화에 의하여 유도되었다. 환경호르몬에 의해 유도된 MCF-7 유방암 세포의 증식은 CJP와 PTP의 처리로 인하여 농도 의존적으로 감소하였으며, 300 mg/ml 농도에서는 대조군과 비교하였을 때 각각 70%와 80% 이상 감소하였다.

Keywords

References

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