Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Sorangium cellulosum Carrying Epothilone Gene Clusters

  • Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Chung, Jin-Woo (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Kim, Ji-Hoon (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB)) ;
  • Lee, Jong-Suk (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB)) ;
  • Kwon, Byoung-Mog (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB)) ;
  • Son, Kwang-Hee (Molecular Cancer Research Center, Korea Research Institute of Bioscience and Biotechnolgoy (KRIBB)) ;
  • Cho, Kyung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • Published : 2008.08.31
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Abstract

Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone biosynthetic gene homologs were screened using a polymerase chain reaction. A migration assay revealed that Sorangium cellulosum KYC3013, 3016, 3017, and 3018 all produced microtubule-stabilizing compounds, and an LC-MS/MS analysis showed that S. cellulosum KYC3013 synthesized epothilone A.

Keywords

References

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