The Study on Antimicrobial and Antifungal Activity of the Wild Seaweeds of Jeju Island

제주 자생 해양식물들의 항균 및 항산화 활성 연구

  • 임지희 (스킨큐어㈜ 생명과학연구소) ;
  • 정광선 (스킨큐어㈜ 생명과학연구소) ;
  • 이종성 (바이오스펙트럼㈜ 생명과학연구소) ;
  • 정은선 (바이오스펙트럼㈜ 생명과학연구소) ;
  • 김대경 (바이오스펙트럼㈜ 생명과학연구소) ;
  • 김영수 (바이오스펙트럼㈜ 생명과학연구소) ;
  • 김용우 (바이오스펙트럼㈜ 생명과학연구소) ;
  • 박덕훈 (바이오스펙트럼㈜ 생명과학연구소)
  • Published : 2008.09.30

Abstract

In this study, we investigated the antimicrobial and antifungal activity from the wild seaweeds of Jeju island. The active ingredients of the seaweeds were prepared by 80% methanol extraction. Antimicrobial and antifungal activity of seaweed extracts was examined. We found that 6 plant extracts among 45 plants, namely, Codium contractum, Undaria pinnatifida, Ishige sinicola, Ishige okamurai, Ishige okamuriai, Ecklonia cava, Hizikia fusiformis, Ulva fasciata, Ulva pertusa, Sargassum siliquastrum, Ecklonia kurome, Gracilaria textorii, significantly inhibited growth of harmful microorganisms. Additionally, according to DPPH assay. 2 plant extracts were found to have antioxidant activities. Taken together, these results suggest the possibility that 11 plant extracts can be utilized as an antimicrobial agent.

본 연구에서는 제주도 해안에 자생하는 45종의 해양식물 추출물에 대한 항균 활성을 조사하였다. 해양식물 추출물 80% 메탄올에서 유효 성분을 추출하여 시료화 하였고 항균환성을 검증하였다. 그 결과, 45종의 해조류 중에서 넓패, 패, 구멍갈파래 등을 포함한 6종의 해양식물이 미생물 생육을 억제하는 결과를 보였다. 항균활성을 갖는 해양식물 중 패, 감태 2종은 항산화 효능도 동시에 가지고 있음을 확인하였다. 이러한 결과를 통해, 본 실험에서 확보된 추출물이 항균 물질로 사용될 수 있는 가능성을 확인하였다.

Keywords

References

  1. M. Nanayama, Antibacterial substances in food, J. Food Microbial., 12, 209 (1996) https://doi.org/10.5803/jsfm.12.209
  2. H. J. Shin, A trend in research and development of natural gardenia pigments, Korean J. Biotechnol. Bioeng., 22(5), 271 (2007)
  3. T. S. Jang, A study on anti-stress effect of aroma essential oils by inhalation, J. Korean Beauty Society, 6(1), 227 (2000)
  4. H. J. Kim, H. W. Lim, S. W. Choi, and C. S. Yoon, Antimicrobial effect of ethanol extract of Dryopteris crassirhizoma nakai on Propionibacterium acnes, J. Soc. Cosmet. Scientists Korea, 32(3), 201 (2006)
  5. P. J. Scheuer, Bioorganic Marine Chemistry. Springer Verlag, 123 (1990)
  6. I. Manou, L. Bouillard, M. J. Devleeschouwer, and A. O. Barel, Evaluation of the preservative properties of Tymus vulgaris essential oil in topically applied formulations under a challenge test, J. Appl. Microbiol., 84, 368 (1998) https://doi.org/10.1046/j.1365-2672.1998.00353.x
  7. G. M. Konig, S. Kehraus, S. F. Seibert, A. Abdel- Lateff, and D. Muller, Natural products from marine organisms and their associated microbes, Chem. Bio. Chem. Soc. Jap., 53, 2020 (1980) https://doi.org/10.1246/bcsj.53.2020
  8. R. J. Andersen and D. E. Williams, Pharmaceuticals from the sea, Chemistry in the marine environment, 55 (2000)
  9. M. S. Ali, M. Jahangir, M. Saleem, M. K. Perves, S. Hameed, and V. U. Ahamad, Metabolites of marine algae collected from karachi-coasts of arabian sea, Natural product Sciences, 6(2), 61 (2000)
  10. Y. K. Min , J. S. Ro, S. K. Lee, and K. W. Chang, Antibacterial effects of Ishige okamurai extract to some oral streptococci, Korean Aca. Dental Health, 21(1), 41 (1997)
  11. Y. Adachi, H. Akamatsu, and T. Horio, The effect of antibiotics on the production of superantigen from Staphylococcus isolated from atopic dermatitis, J. Dermatol. Sci., 28, 76 (2002) https://doi.org/10.1016/S0923-1811(01)00143-8
  12. D. J. Ahn, Y. S. Kwak, M. J. Kim, and J. C. Lee, Screening of herbal plant extracts showing antimicrobial activity against some food spoilage and pathogenic microorganisms, Korean J. Medicinal Crop Sci., 8(2), 109 (2000)
  13. S. Y. Jang, S. Y. Yu, and S. D. Kim, Antifungal activity of plant extracts against Pityrosporum ovale and Candida albicans, Kor. J. Pharmacogn., 34, 303 (2003)
  14. V. N. Scott, Safety considerations for new generation refrigerated foods, Dairy Food Environ. Sanitation, 8(5), 8 (1988)
  15. S. Marklud and G. Marklud, Involvement of the superoxide anion radical in the antoxidation of pyrogallol and a convenient assay for superoxide dismutase, Eur. J. Biochem., 47, 469 (1974) https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  16. Microbiol. Soiety Korea, Exepriment of microbiology, 121, Academybook, Korea (1987)
  17. Korean Research Institute of Chemical Technology, A Study on the Establishment of Pharmacological Screening Program (III), 153, Ministry of education, science and technology, Korea (1989)
  18. A. M. Layton, A review in the treatment of acne vulgaris, Int. J. Clin. Pract., 60, 64 (2004) https://doi.org/10.1111/j.1368-5031.2005.00695.x