KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Urease Inhibition and Flocculating Activity of Concentrated Aloe vera Gel by Using Ultrafiltration Process

한외여과 알로에 농축액의 Urease 저해 및 무기물 응집 활성

  • 백진홍 ((주)김정문 알로에 과학연구소) ;
  • 김성아 ((주)김정문 알로에 과학연구소) ;
  • 이신영 (강원대학교 생물공학과)
  • Published : 2008.06.30
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Abstract

For physiological function of aloe concentrate by ultrafiltration (UF) process, jack bean urease inhibitory activity and bentonite flocculating activity of UF aloe concentrate was investigated and compared with fresh aloe gel. Urease inhibitory activity of UF aloe concentrate ranged from 87 to 90% in 1 mL sample. Also, urease inhibitory activity of UF aloe concentrate increased about 10% by heat treatment showing the heat stability. From Lineweaver-Burk plot for UF aloe concentrate, urease inhibition pattern indicated general non-competitive inhibition. From flocculation test of UF aloe concentrate about 1% (w/v) bentonite suspension, maximum flocclulating activity of 97% was obtained at 0.5 mL addition of UF aloe concentrate/ 5 ml bentonite suspension. However, flocculating activity of 81% was obtained at 1 mL addition of UF aloe concentrate/ 5 mL bentonite suspension, which was typical flocculating behavior of polymers with re-dispersion at overdose area. FT-IR spectra of UF aloe concentrate showed the characteristic patterns of $\beta$-binding polysaccharide and less deacetylation indicating higher level of bioactive polysaccharide content.

Keywords

References

  1. Grindlay, D. and T. Reynolds (1986), The Aloe vera phenomenon. A review of the properties and modem uses of the leaf parenchyma gel. J. Ethnopharmacol. 117-151 https://doi.org/10.1016/0378-8741(86)90085-1
  2. Reynolds, T. and A. C. Dweck (1999), Aloe vera leaf gel: a review update J. Ethnopharmacol. 68, 3-37 https://doi.org/10.1016/S0378-8741(99)00085-9
  3. Blitz, J. J., Smith, J. W., and Gerard, J. R. (1966), Aole vera gel in peptic ulcer therapy: preliminary report. J. Am. Osteopath. Assoc. 62, 731-735
  4. Borrelli, F., and Izzo, A. A. (2000), The plant kingdom as a source of anti-ulcer remedies. Phytotherapy Research 14(8), 581-591 https://doi.org/10.1002/1099-1573(200012)14:8<581::AID-PTR776>3.0.CO;2-S
  5. Langmead, L., Feakins, R. M., and Goldthorpe, S. (2004), Randomized, double-blind, placebo-controlled trial of oral Aloe vera gel for active ulcerative colitis. Aliment Pharmacol Ther. 19, 739-48 https://doi.org/10.1111/j.1365-2036.2004.01902.x
  6. Blaser, M. J. (1992), Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102, 720-727 https://doi.org/10.1016/0016-5085(92)90126-J
  7. Parsonnet, J., G. D. Friedman, D. P. Vandersteen, Y. Chang, J. H. Vogelman, N. Orentreich, and R. K. Sibley. (1991), Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325, 1127-1131
  8. Cover, T. L., and Blazer, M. J. (1992), Helicobacter pylori and gastroduodenal ulcer disease. Annu. Rev. Med. 43, 135-145 https://doi.org/10.1146/annurev.me.43.020192.001031
  9. Ciurli, S., Benini, S., Rypniewski, W. R., Wilson, K. S., Miletti, S., and Mangani, S. (1999), Structural properties of the nickel ions in urease: novel insight into the catalytic and inhibition mechanism. Coordination Chemistry Reviews 190-192, 331-335 https://doi.org/10.1016/S0010-8545(99)00093-4
  10. Cesareo, S. D., and Langton, S. R. (1992), Kinetic properties of Helicobacter pylori urease compared with jack bean urease. FEMS Microbiol Lett. 78(1), 15-21
  11. Lee S. Y., Min B. J., and Kang T. S. (1998), flocculating activity of the mucilage extracted from Aloe vera Linne. Korean J. Biotechnol Bioeng. 13, 540-546
  12. Rossi, S. Luckham, P. F., Zhu, S., Briscoe, B. J., and Tadros, TH. F. (1997), Influence of low molecular weight polymers on the rheology of bentonite suspensions. Revue de L'lnstitut Francaid Du Petrole. 52(2), 199-206 https://doi.org/10.2516/ogst:1997019
  13. Miki, B. L., Hung poon, N., James, A. P., and Seligy, V. L. (1982), Possible mechanism for flocculation interactions governed by gene FLOI in Saccharomyces cerevisiae. Journal of Bacteriology. 150(2), 878-889
  14. Toeda, K. and Kurane, R. 1991. Microbial flocculant from Alkaligenes cupidus KT201. Agric. BioI. Chem. 55, 2793-2799 https://doi.org/10.1271/bbb1961.55.2793
  15. Sumasundran, P. and Yu, X. (1994), Flocculation/dispersion of suspensions by controlling adsorption and conformation of polymers and surfactants. Advances in Colloid and Inteiface Science. 53, 33-49 https://doi.org/10.1016/0001-8686(94)00208-8
  16. Dengis, P. B., Nellissen, L. R., and Rouxhet, P. G. (1995), Mechanisms of yeast flocculation: Comparison of top- and bottom-fermenting strains. Appl. Environ. Microbiol. 61(2), 718-728
  17. Zang, J. Liu, Z., Wang, S., and Jiang, P. (2002), Characterization of a bioflocculant produced by the marine Mycobacterium nannocystis sp. NU-2. Appl. Microbiol. Biotechnol. 59, 517-522 https://doi.org/10.1007/s00253-002-1023-7
  18. Roussy, J., Van Vooren, M., and Guibal, E. (2004), Chitosan for the coagulation and flocculation of mineral colloids. J. Dispersion Sci. Technol. 25(5), 633-677
  19. McAnalley, B. H. and Prairie, G. (1988), Process for preparation of aloe products, produced thereby and components thereof. US patent 4,735,935
  20. Baek, J. H., S. A. Kim, and S. Y. Lee (2008), Concentration of fresh gel from Aloe vera L. by using ultramtration process, Korean J. Biotechnol. Bioeng. 23(2), 169-176
  21. Mobley, H. L. T., Cortesia, M. J., Rosenthal, L. E., and Jones, B. D. (1988), Characterization of urease from Campylobacter pylori. Journal of Clinical Microbiology 26, 831-836
  22. Segel, I. H. (1975), Enzyme Kinetics, A Wiley-lnterscience Publication, New York, 100-160, 161-226
  23. Eckenfelder, W. W. and Ford, D. L. (1970), Water Polurion Control: Experimental Procedure for Process Design. Jenkins Pub. Co. The pemberton Press, Austin, USA.
  24. Poirier, M. R. (2001), Evaluation of flocculation and filtration procedures applied to WSRC sludge. WSRC-TR-2001-002I3, 16
  25. Oomori, E. (1986), Polymer Flocculant (in Japanese). Kobunshi Kanko-Kai, Kyoto, Japan, pp17-19
  26. Yu, X. and Somasundaran, P. (1996), Kinetics of polymer conformational changes and its role in flocculation. J. Colloid Interface Sci. 178, 770-774 https://doi.org/10.1006/jcis.1996.0176
  27. Tjpangandjara, K. F. and Somasundaran, P. (1992), Effects of the conformation of poly acrylic acid on the dispersion-flocculation of alumina and kaolinite fmes. Advanced Powder Technol. 3(2), 119-127 https://doi.org/10.1016/S0921-8831(08)60678-0
  28. McAnalley, B. H. (1993), Process for preparation of aloe products. European Patent WO89/o6539
  29. Dea, I. C. M., and Clark, A. H. (1986), Effect of the galactosesubstitution patterns on the interaction properties of galactomannans. Carbohydrate Research 147, 275-294 https://doi.org/10.1016/S0008-6215(00)90637-2
  30. Blafkova, P., Synytsya, A., and Copikova, J. (2004), Chitin-glucan complex from Agaricus blazei, a potential raw material for production of food additives. Czech J. Food Sci. 22(special issue), 231-234 https://doi.org/10.17221/10668-CJFS
  31. Yaron, A. (1993), Characterization of Aloe vera before and after autodegradation and stabilization of the natural fresh gel. Phytother. Res. 7, S11-S13 https://doi.org/10.1002/ptr.2650070706