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Effect of Some Food Preservatives on the Lipolytic Activity of Beef Luncheon Fungi

  • 발행 : 2008.09.30

초록

Beef luncheon meat is one of the most popular meals in several countries in the world including Egypt. Thirty one fungal species and 3 species varieties were recovered from 30 samples of beef luncheon meat collected from different supermarkets in Qena. Alternaria, Aspergillus, Emericella, Mucor, Mycosphaerella, Penicillium and Rhizopus were the most common genera on the two types of media. From the above genera, the most prevalent species were Alternaria alternate, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Emericella nidulans, Mucor racemosus, Mycosphaerella tassiana, Penicillium chrysogenum and Rhizopus stolonifer. Screening of fungi for their abilities to produce lipase enzyme showed that, ten isolates represented 32.26% of total isolates appeared high lipase production, while sixteen isolates (51.61%) were moderate and 5 isolates (16.13%) were low producers. Aspergillus niger, Fusarium oxysporum and Nectria haematococca produced the highest amount of lipase enzyme, so these fungi were used in further studies. The incorporation of five food preservatives (Disodium phosphate, sodium benzoate, citric acid, potassium sorbate and sodium citrate) individually in the culture medium of lipase production exhibited an inhibitive effect on the mycelial growth and enzyme production by Aspergillus niger, Fusarium oxysporum and Nectria haematococca.

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참고문헌

  1. Alberghina, L., Schmid, R. D. and Verger, R. 1991. Lipases: structure, mechanism and genetic engineering. Wiley-VCH, Weinheim
  2. Al-Doory, Y. 1980. Laboratory medical mycology. Lea and Febiger, Philadelphia Kimpton publishers, London
  3. Andersen, S. J. 1995. Compositional changes in surface mycoflora during ripening of naturally fermented sausages. J. Food Protect. 58:426-429 https://doi.org/10.4315/0362-028X-58.4.426
  4. Aymerich, T., Picouet, P. A. and Monfort, J. M. 2008. Decontamination technologies for meat products. Meat Science 78:114- 129 https://doi.org/10.1016/j.meatsci.2007.07.007
  5. Balashev, K., Jensen, T. R., Kjaer, K. and Bjornholm, T. 2001. Novel methods for studying lipids and lipases and their mutual interaction at interfaces: Part I. Atomic force microscopy. Biochimie 83:387-397 https://doi.org/10.1016/S0300-9084(01)01264-0
  6. Beuchat, L. R. 1981. Combined effects of solutes and food preservatives on rates of inactivation of and colony formation by heated spores and vegetative cells of molds. Appl. Environ. Microbiol. 41:472-477
  7. Bornscheuer, U.T. 2000. Enzymes in lipid modification. Wiley- VCH, Weinheim
  8. Cardenas, J., Alvarez, E., De Castro-Alvarez, M. S., Sanchez- Montero, J. M., Valmaseda, M., Elson, S. W. and Sinisterra, J. V. 2001. Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases. J. Mol. Catal. B: Enzym. 14:111-123 https://doi.org/10.1016/S1381-1177(00)00244-7
  9. Casado, M. J. M., Borras, M. A. and Aguilar, R. V. 1991. Fungal flora present on the surface of cured Spanish ham. Methodological study for its isolation and identification. Fleischwirtschaft 71:1300-1302
  10. Coma, V. 2008. Bioactive packaging technologies for extended shelf life of meat-based products. Meat Science 78:90-103 https://doi.org/10.1016/j.meatsci.2007.07.035
  11. Geornaras, I., Skandamis, P. N., Belk, K. E., Scanga, J. A., Kendall, P. A., Smith, G. C. and Sofos, J. N. 2006. Post-processing application of chemical solutions for control of Listeria monocytogenes, cultured under different conditions, on commercial smoked sausage formulated with and without potassium lactate- sodium discetate. Food Microbiol. 23:762-771 https://doi.org/10.1016/j.fm.2006.01.008
  12. Grazia, L., Romano, P., Bagni, A., Roggiani, D. and Guglielmi, G. 1986. The role of moulds in the ripening process of salami. Food Microbiol. 3:19-25 https://doi.org/10.1016/S0740-0020(86)80021-1
  13. Han, J. H. 2005. Antimicrobial packaging systems. In: Innovations in Food Packaging. pp. 81-107. Ed. J. H. Han, Elsevier Academic Press, Amsterdam
  14. Hwang, H. J., Vogel, R. F. and Hammes, W. P. 1993. Entwicklung von Schimmelpilzkulturen für die Rohwurstherstellung Charakterisierung der Stämme und toxikologische Bewertung. Fleischwirtschaft 73:89-92
  15. Jesenska, Z. 1987. Mikroskopické huby v požívatinácha v krmivách. Alfa, Bratislava: 36-37
  16. Laciakova, A. and Laciak, V. 1994. Moznosti eliminacie mikroskopickych vláknitych húb dezinfek nymi prostriedkami. Vet. Med. (Czech) 39: 723-731
  17. Leistner, L. 1986. Mold-ripened foods. Fleischwirtschaft 66: 1385-1388
  18. Martinelle, M., Holmquist, M. and Hult, K. 1995. On the interfacial activation of Candida antarctica lipase A and B as compared with Humicola lanuginosa lipase. Biochim. Biophys. Acta 1258:272-276 https://doi.org/10.1016/0005-2760(95)00131-U
  19. Mateos Diaz, J. C., Rodriguez, J. A., Roussos, S., Cordova, J., Abousalham, A., Carriere, F. and Baratti, J. 2006. Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures. Enzyme Microb. Technol. 39:1042-1050 https://doi.org/10.1016/j.enzmictec.2006.02.005
  20. Mizakova, A., Pipova, M. and Turek, P. 2002, The occurrence of moulds in fermented raw meat products. Czech J. Food Science 20:89-94
  21. Nagy, V., Toke, E. R., Keong, L. C., Szatzker, G., Ibrahim, D., Omar, I. C., Szakacs, G. and Poppe, L. 2006. Kinetic resolutions with novel, highly enantioselective fungal lipases produced by solid state fermentation. J. Mol. Catal. B: Enzym. 39:141-148 https://doi.org/10.1016/j.molcatb.2006.01.012
  22. Ostry, V. 2001. Vyskyt plísní v mase a masnych vyrobcích (I). Vliv na zdraví lov ka. Maso 12:20-24
  23. Quintavalla, S. and Vicini, L. 2002. Antimicrobial food packaging in meat industry. Meat Science 62:373-380 https://doi.org/10.1016/S0309-1740(02)00121-3
  24. Sharma, R., Chisti, Y. and Banerjee, U. C. 2001. Production, purification, characterization and applications of lipases. Biotechnol. Adv. 19: 627-662 https://doi.org/10.1016/S0734-9750(01)00086-6
  25. Zaky, Z. M., Ismail, M. A. and Refaie, R. S. 1995. Aspergillus flavus and aflatoxins residues in luncheon meat. Assiut Vet. Med. 33:114-118