한국양식학회지 (Journal of Aquaculture)

한국양식학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지

동해 연안에 서식하는 성게의 형태변이와 미토콘드리아 유전자 분석

Morphological Variation and Partial Mitochondrial Sequence Analysis of Echinoid Species from the Coasts of the East Sea

  • 신지혜 (강릉대학교 해양생명공학부) ;
  • 김성규 (강릉대학교 해양생명공학부) ;
  • 김영대 (국립수산과학원 동해수산연구소) ;
  • 손영창 (강릉대학교 해양생명공학부)
  • Shin, Ji-Hye (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Kim, Sung-Gyu (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Kim, Young-Dae (East Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Sohn, Young-Chang (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 발행 : 2008.08.25
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초록

성게의 형태학적 분류는 그것의 형질적 변이에 의하여 어려움이 많다. 본 연구에서는 말똥성게, 둥근성게, 보라성게, 분홍성게와 동해안에서 포획된 미확인 성게 4종의 형태형질 비교와 계통유연관계를 조사하였다. 성게의 생식소로부터 genomic DNA를 분리한 후, PCR 방법을 통하여 mitochondrial 12S rDNA 유전자의 염기서열을 분석하였다. 둥근성게과의 말똥성게, 둥근성게, 만두성게과의 보라성게, 주발성게과의 분홍성게의 mitochondrial 12S rDNA의 염기서열은 미확인 성게종들의 그것과 85.9-93.9%의 상동성을 나타내었다. 한편, 미확인 성게종들은 새치성게의 mitochondrial 12S rDNA의 일부 염기서열과 99.8%의 높은 상동성을 보였으며, 각 개체의 mitochondrial 12S rDNA를 통한 분자계통수 분석에 의해서 미확인 성게들은 새치성게의 형태적 변이로 판단된다.

Morphological classification of echinoid species has many difficulties because of their phenotypic variations. In the present study, we analyzed morphotypes and partial mitochondrial 12S rDNA sequences of four sea urchin species classified as Pseudocentrotus depressus, Anthocidaris crassispina, Hemicentrotus pulcherrimus and Strongylocentrotus nudus, and unidentified four species collected from the coasts of the East sea. Their genomic DNAs were extracted from gonads and mitochondrial 12S rDNA sequences were amplified by the polymerase chain reaction (PCR) method. The sequence identities among the known four sea urchin species were 87.4-95.6%. The sequence identities among the unidentified four species were 99.4-99.6% and showed the highest homology to S. intermedius(99.8%). Thus, our phylogenetic tree indicates that the unidentified four species belong to S. intermedius.

키워드

참고문헌

  1. Bazhin, A. G., 1998. The Sea Urchin Genus Strongylocentrotus in the Seas of Russia Taxonomy and Range. Balkema, Rotterdam, pp. 563-566
  2. Cummings, A. and R. Kavlock, 2005. A systems biology approach to developmental toxicology. Reprod. Toxicol., 19, 281-290 https://doi.org/10.1016/j.reprotox.2004.10.001
  3. Kessing, B. D., 1990. Concurrent speciation in template sea urchin lineages. Pac. Sci., 44, 188-195
  4. Kwon, H. S., J. W. Pyo and S. Shin, 2004. Sequence analysis of mitochondrial 12S rDNA gene and molecular phylogeny of Korea echinoid species. Sahmyook Univ. J. 39, 341-348
  5. Lee, J. J., B. K. Kim, S. K. Kang, S. C. Chung, K. W. Lee and K. S. Choi, 2000. Reproductive ecology and genetic variations in the sea urchins Anthocidaris crassispina and Hemicentrotus pulcherrimus in the Cheju Coast. J. Aquacult., 13, 129-135
  6. Lee, Y. H., 2003. Molecular Phylogenies and divergence times of sea urchin species of Strongylocentrotidae, Echinoida. Mol. Biol. Evol., 20, 1211-1221 https://doi.org/10.1093/molbev/msg125
  7. Levine, M. and E. H. Davidson, 2005. Gene regulatory networks for development. Proc. Natl. Acad. Sci. USA., 102, 4936-4942
  8. Littlewood, D. T. and A. B. Smith, 1995. A combined morphological and molecular phylogeny for sea urchins (Echinoidea: Echinodermate). Philos. Trans. R. Soc. Lond. B. Biol. Sci., 347, 213-234 https://doi.org/10.1098/rstb.1995.0023
  9. Matsubara, M., Komatsu M., Araki T., Asakawa S., Yokobori S., Watanabe, K. and Wada, H. 2005. The Phylogenetic status of Paxillosida (Asteroidea) based on complete mitochondrial DNA sequences. Mol. Phylogenet. Evol., 36, 598-605 https://doi.org/10.1016/j.ympev.2005.03.018
  10. McMillan, W. O., R. A. Raff and S. R. Palumbi, 1992. Population genetic consequences of development evolution in sea urchins (Genus Heiocidaris). Evolution, 46, 1299-1312 https://doi.org/10.2307/2409938
  11. Pyo, J. W., H. S. Kwon and S. Shin, 2005. Sequence analysis of three Echinoderm species and phylogenetic relation ship of Korean Echinoderms based on mitochondrial 12S rDNA gene. J. Life Sci., 13, 38-46
  12. Saitou, N. and M. Nei, 1987. The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol. Biol. Evol., 4, 406-425
  13. Sambrook, J. and D. W. Russell, 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring, NY: Cold Spring Harbor Laboratory Press
  14. Shin, S., 2000. New record of two Echinoids (Echinodermate, Echinoidea) in Korea. Kor. J. Syst. Zool., 16, 219-226
  15. Takata, H. and T. Kominami, 2003. Behavior of differentiation process of pigment cells in a tropical sea urchin Echinometra mathaei. Dev. Growth Differ., 45, 473-483 https://doi.org/10.1111/j.1440-169X.2003.00714.x
  16. Takata, H. and T. Kominami, 2004. Behavior of pigment cells closely correlates the manner of gastrulation in sea urchin embryos. Zoolog. Sci., 21,1025-1035 https://doi.org/10.2108/zsj.21.1025
  17. Tatarenko, D. E. and A. B. Poltaraus, 1991. Classification of the sea urchins Strongylocentrotus echinoides and S. sachalinicus as S. pallidus on the strength of genome comparison. Biol. Morya Mar. Biol., 3, 69-75
  18. Tatarenko, D. E. and A. B. Poltaraus, 1992. Affiliation of the sea urchins Strongylocentrotus echinoides and S. sachalinicus to the species S. pallidus based on a comparison of their genomes. Russ. J. Mar. Biol., 17, 168-172
  19. Thomas, W. K., J. Maa and A. C. Wilson, 1989. Shifting constraints on tRNA genes during mitochondrial DNA evolution in animals. New. Biol., 1, 93-100
  20. Vaitilingon, D., I. Eeckhaut, D. Fourgon and M. Jangoux, 2004. Population dynamics, infestation and host selection of Vexilla vexillum, an ectoparasitic muricid of echinoids, in Madagascar. Dis. Aquat. Organ., 61, 241-255 https://doi.org/10.3354/dao061241
  21. 신숙, 노분조. 1996. 한국동식물도감. 제 36권 동물편(극피동물), 교육부, 서울, 775 pp
  22. NFRDI. 2005. A Study on construction of seaweed forest in the East Sea. Final Report on Fisheries Life Sciences and Aquaculture Sciences. National Fisheries R & D Institute, pp. 5-50
  23. NFRDI. 2006. A Study on Construction of Seaweed Forest in the East Sea. Final Report on Fisheries Life Sciences and Aquaculture Sciences. National Fisheries R & D Institute, pp. 521-696