Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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BONE HEALING CAPACITY OF THE COLLAGEN BONE FILLER ($TERUPLUG^{(R)}$) AND RHBMP-2 IN THE RABBIT CRANIUM DEFECT

가토 두개골 결손부에 이식된 Collagen bone filler ($TERUPLUG^{(R)}$) 및 rhBMP-2의 골치유 능력

  • Kim, Ju-Hoon (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Chul-Hwan (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Kyung-Wook (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
  • 김주훈 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 김철환 (단국대학교 치과대학 구강악안면외과학교실) ;
  • 김경욱 (단국대학교 치과대학 구강악안면외과학교실)
  • Published : 2008.04.30
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Abstract

Absorbable atelo-collagen sponge $TERUPLUG^{(R)}$, Termo Co. Tokyo, Japan) is inserted in the extraction wound where alveolar bone is exposed. It protects wounds and promotes the formation of granulation. This is made of atelo-collagen, to minimize antigenicity, which is cross-linked by heat treatment for biocompatibility. $TERUPLUG^{(R)}$ consists of between 85 and 95 % of collagen type I and between 5 to 15 % of collagen type III. The raw material for the collagen is derived from bovine skin. It features a sponge block design and is shaped for easy insertion in the extraction wound. This study was designed to find out the bone healing capacity of $TERUPLUG^{(R)}$. We implanted $TERUPLUG^{(R)}$ (experimental group I) and $TERUPLUG^{(R)}$ with rhBMP-2 (experimental group II) in the rabbit cranium defect and then histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, a lot of the newly formed collagen fibers around material of the experimental group I implanted $TERUPLUG^{(R)}$ were observed. But, in the experimental group II implanted $TERUPLUG^{(R)}$ with rhBMP-2, a little of newly formed collagen fibers around material were observed. The cell proliferating activity and apoptosis of the experimental group I, II was positive in and around the implanted material. 2. In the 8 weeks, the amount of newly formed and matured bone in the experimental group II was more observed than the experimental group I and control group. The results of this study indicate that absorbable atelo-collagen sponge ($TERUPLUG^{(R)}$) is relatively favorable bone void filler with biocompatibility and has the better bone healing capacity in case of application with rhBMP-2.

Keywords

References

  1. Boyne PJ: Induction of bone repair by various bone grafting materials, hard tissue growth, repair and remineralization. Ciba Found Symp 1973;11:121
  2. Papay FA, Morales L, Ahmed OF, et al.: Comparison of ossification of demineralized bone, hydroxyapatite, Gelfoam, and bone wax in cranial defect repair. J Craniofac Surg 1996;7:347 https://doi.org/10.1097/00001665-199609000-00006
  3. Anthony G. Sclar: Soft tissue and Esthetic Considerations in Implant Dentistry. Quintessence Publishing Co. Inc 2003;76-77
  4. Mark E. K. Wong: The Biology of Alveolar Healing Following the removal of Impacted Teeth. WB SAUNDERS 25-45, 1993
  5. Hunt TK, Knighton DR, Thakral KK, et al.: Cellular control of repair; Soft and Hard tissue Repair. Praeger, New York, 1984
  6. Orgill D, Demling RH: Current concepts and approaches to wound healing. Crit care Med 1988;16:899 https://doi.org/10.1097/00003246-198809000-00016
  7. Gustafson GT: Ecology of wound healing in the oral cavity. Scand J Haematol Suppl 1984;40:393
  8. Lekovic V, Kenney EB, Weinlaender M, et al.: A bone regeneration approach to alveolar ridge maintenance following tooth extraction. J Periodontol 1997;68:563-570 https://doi.org/10.1902/jop.1997.68.6.563
  9. Lang N, Becker W, Karring T: Alveolar bone formation. Textbook of Clinical Periodontology and Implant Dentistry, ed 3. Munksgaard, 906-932, 1998
  10. Clokie C: Material presented at the scientific sessions of the Canadian Association of Oral and Maxillofacial Surgery, Vancouver, 16 July 1998
  11. Olson RAJ, Roberts DL, Osbon DB: A comparative study of polylactic acid, gelfoam, and surgicel in healing extraction sites. Oral Surg Oral Med Oral Path 1982;53:441 https://doi.org/10.1016/0030-4220(82)90453-4
  12. Bhaskar SN, Cutright DE, Knapp MJ, et al.: Tissue reaction to intrabony ceramic implants. Oral Surg Oral Med Oral Path 1971;31:282 https://doi.org/10.1016/0030-4220(71)90086-7
  13. Prokic B, Carranza FA, Kenny EB, et al.: Comparative clinical study of porous hydroxyapatite and decalcified freeze-dried bone in human periodontal defects. J Periodontol 1990;61:399 https://doi.org/10.1902/jop.1990.61.7.399
  14. Moller JF, Peterson JK: Efficacy of a fibrin sealant on healing of extraction wounds. Int J Oral Maxillofacial Surgery 1988;17:142 https://doi.org/10.1016/S0901-5027(88)80170-X
  15. Boyes-Varley JG, Cleaton-Jones PE, Lownie JF: Effect of a topical drug combination on the early healing of extraction sockets in the vervet monkey. Int J Oral Maxillofac Surg 1988;17:138 https://doi.org/10.1016/S0901-5027(88)80169-3
  16. Lekovic V, Camargo PM, Klokkevold PR, et al.: Preservation of alveolar bone in extraction sockets using bioabsorbable membranes. J periodontol 1998;69:1044-1049 https://doi.org/10.1902/jop.1998.69.9.1044
  17. Warner K, Gotfredsen K, Karring T, et al.: Guided tissue regeneration ensures osseointegration of dental placed in extraction sockets. An experimental study in monkeys. Clin Oral Implants Res 1991;2;166-171 https://doi.org/10.1034/j.1600-0501.1991.020402.x
  18. Becker W, Becker B, Handelsman M, et al.: Guided tissue regeneration for implants placed in extraction sockets. An experimental study in dogs. J Periodontol 1991;62;703-709 https://doi.org/10.1902/jop.1991.62.11.703
  19. Roberts BW: Bone induction in healing tooth sockets using piezo-electric foil. J Dent 1982;10:243 https://doi.org/10.1016/0300-5712(82)90056-2
  20. Black J: Electrical stimulation of hard and soft tissues in animal models. Clin Plas Surg 1985;12:243
  21. Battistone GC, Posey WR, Barone JJ, et al.: Zinc and bone healing; The effect of zinc cysteamine-N-acetic acid on the healing of extraction wounds in rats. Oral Surg Oral Med Oral Path 1972; 34:704 https://doi.org/10.1016/0030-4220(72)90358-1
  22. Konishi J: Animal Study of Tooth Extraction Wound Healing Using Collagen Sponge Matrix. SEITAIZAIRYOU, 1998;16: 266-275
  23. Glowacki J, Mulliken JB: Induced osteogenesis for repair and construction in the craniofacial region. Plast Reconstr Surg 1980;65:553 https://doi.org/10.1097/00006534-198005000-00001
  24. Glowacki J, Kaban LB, Mulliken JB: Treatment of jaws defects with demineralized bone implants. J Oral Maxillfac Surg 1982;40:623 https://doi.org/10.1016/0278-2391(82)90109-4
  25. Glowacki J, Mulliken JB: Demineralized bone implants. Clin Plast Surg 1985;12:233
  26. Burstein AH, Einhorn TA, Kopman CR, et al.: The healing of segmental bone defects induced by demineralized bone matrix. J Bone Joint Surg 1984;66:274 https://doi.org/10.2106/00004623-198466020-00015
  27. Masters DH: Implants. Bone and bone substitutes. Calif Dent Assoc J 1988;16:56
  28. Koide M: Characterization of collagen spongy materials. J Biomed Mater Res 1993;27:79-87 https://doi.org/10.1002/jbm.820270111
  29. Konishi J: Composition of collagen materials. Jpn J Artif Organs 1989;18:155-158
  30. Hiroshi N, Hitoshi U, Hiroshi K, et al.: Computed Tomographic Examination fo Bone Healing after Placement of Collagen Sponge Matrix in the Extraction Site. J Osaka Odontol Society 2001;64:369-374
  31. Kaihara S, Sonobe J, Iizuka T, et al.: Experimental study of bone inducing abillity of rH BMP-2 with atelo-collagen sponge as a carrier. Jpn. J Oral Maxillofac Surg 2003;49:241-245
  32. Fujimura K, Bessho K, Kusumoto K, et al.: Experimental studies on bone inducing activity of composites of atelopeptide type I collagen as a carrier for ectopic osteoinduction by rhBMP-2. Biochem Biophys Res Commun 1995;208:316-322 https://doi.org/10.1006/bbrc.1995.1340
  33. Urist MR: Bone Formation by autoinduction. Science 1965;150:893-899 https://doi.org/10.1126/science.150.3698.893
  34. Urist MR, Strates BS: Bone morphogenic protein. 1971;1392-1406 https://doi.org/10.1177/00220345710500060601
  35. Reddi AH: Initiation and promotion by bone morphogenic proteins. J Bone Min Res 1993;8:449-502 https://doi.org/10.1002/jbmr.5650081302
  36. Hammonds RG, Schwall R, Dudley A, et al.: Bone inducing activity of mature BMP-2B produced from a hybrid BMP-2A/2B precursor. Mol Endocrinol 1991;5:149-155 https://doi.org/10.1210/mend-5-1-149
  37. K. Bessho, Y. Konishi, S. Kaihara, et al.: Bone induction by Escherichia coli-derived recombinant human bone morphogenetic protein-2 compared with Chinese hamster ovary cell-derived recombinant human bone morphogenetic protein-2. British Journal of Oral and Maxillofacial Surgery 2000;38:645-649 https://doi.org/10.1054/bjom.2000.0533
  38. Wang EA, Rosen V, Cordes P, et al.: Purification and characterization of other distinct bone-inducing factors. Proc Natl Acad Sci USA 1988;85:9484-9488 https://doi.org/10.1073/pnas.85.24.9484
  39. Bessho K: Ectopic osteoinductive difference between purified bovine and recombinant human bone morphogenetic protein. In: Lindholm TS, ed. Bone Morphogenetic Proteins: Biology, Biochemistry and Reconstructive Surgery. Austin: RG Landes, 105-111, 1996
  40. Wang EA, Rosen V, D'Alessandro JS, et al.: Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 1990;87:2220-2224 https://doi.org/10.1073/pnas.87.6.2220
  41. Yamaguchi A, Katagiri T, Ikeda T, et al.: Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol 1991;113:68-687