Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제34권4호
  • /
  • Pages.405-411
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  • 2008
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

정상 치은 조직에서 배양된 각화세포에서 Cyclosporine A에 의한 MMP-1, TIMP-1, $TGF-{\beta}_1$의 발현에 관한 연구

THE mRNA EXPRESSION OF MMP-1, TIMP-1, $TGF-{\beta}_1$ IN GINGIVAL KERATOCYTES FROM GINGIVAL HYPERPLASIA INDUCED BY CYCLOSPORINE A

  • 강학수 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 이재선 (한양대학교 의과대학 의생명연구소) ;
  • 빙정호 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 임재중 (한양대학교 의과대학 치과학교실 치과교정과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Kang, Hag-Soo (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University) ;
  • Lee, Jae-Sun (Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Bing, Jung-Ho (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University) ;
  • Park, Chang-Joo (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University) ;
  • Im, Jae-Jung (Division of Orthodontics/Department of Dentistry, College of Medicine, Hanyang University) ;
  • Hwang, Kyung-Gyun (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Division of Oral and Maxillofacial Surgery/Department of Dentistry, College of Medicine, Hanyang University)
  • 발행 : 2008.08.29
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초록

Purpose : Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and $TGF-{\beta}_1$ and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and $TGF-{\beta}_1$ were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and $TGF-{\beta}_1$ were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or $TGF-{\beta}_1$ on gingival collagen metabolism in patients treated with CsA.

키워드

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