한국가금학회지 (Korean Journal of Poultry Science)

  • 제35권1호
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  • Pages.9-13
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  • 2008
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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Rapid Detection and Discrimination of the Three Salmonella Serotypes, S. Pullorum, S. Gallinarum and S. Enteritidis by PCR-RFLP of ITS and fliC Genes

  • Cha, Se-Yeoun (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Jang, Du-Hee (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Sang-Min (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, Jong-Beom (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Jang, Hyung-Kwan (Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
  • 발행 : 2008.03.31
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초록

양계 산업에서 살모넬라에 의한 질병들 중 가장 중요한 원인체는 S. Gallinarum, S. Purollum, S. Enteritidis로 간주된다. 생화학적 검사에 의한 직접적인 균 분리.동정과 같은 이들 질병에 대한 종래의 진단법은 많은 시간이 소요되며, 특이성 또한 낮다. 본 연구는 3종의 살모넬라균에 의해 야기되는 이들 질병에 대한 빠르고 정확한 진단을 위한 효율적인 진단법에 초점을 두었다. 먼저 종래의 생화학적 검사를 대신하여 새로 고안된 ITSF/ITSR PCR primer를 이용하여 살모넬라균임을 확인하였으며, 증폭된 phase 1 flagellin (fliC) 유전자를 BpmI 또는 BfaI 제한 효소로 처리하여 S. Gallinarum, S. Purollum, S. Enteritidis를 상호 용이하게 감별하였다. 이상의 결과는 3종의 살모넬라균을 효율적으로 진단하기 위해 신속하게 검출하고 감별할 수 있는 유용한 진단법임을 알 수 있었다.

Salmonella enterica serotype gallinarum biovar Gallinarum or Pullorum and Salmonella enterica serotype Enteritidis are the most important diseases in poultry industry. Transitional diagnosis methods of these diseases such as direct isolation and identification by a biochemical test are time consuming with low specificity. In this study, we have focused on the suitable procedure for the rapid and accurate diagnosis of diseases derived from the three Salmonella strains. We initially confirmed Salmonella species by PCR using a specific ITSF/ITSR primer pair instead of biochemical test, and then the PCR-amplified phase 1 flagellin (fliC) using a specific fliCF/fliCR primer pair was digested with a restriction endonuclease, Bpm I and/or Bfa I, to discriminate among S. Pullorum, S. Gallinarum, and S. Enteritidis. We found that these methods could be applied to field isolates of the three Salmonella strains to detect and to discriminate rapidly for convenient diagnosis.

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참고문헌

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