Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

  • Ahn, Ji-Hye (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Jae-Hwan (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Su-Youn (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Woo-Young (BioFood Team, Korea Food & Drug Administration) ;
  • Park, Sun-Hee (BioFood Team, Korea Food & Drug Administration) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2008.06.30
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Abstract

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

Keywords

References

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