Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Regulatory Roles of Chrysanthemum zawadskii Roots in Nuclear Factor E2-related Factor 2/Antioxidant Response Element Pathway

  • Kang, Hye-Sook (Food Science Institute, Institute of Biomedical Engineering, School of Food and Life Sciences, College of Biomedical Science and Engineering, Inje University) ;
  • Park, Min-Ji (Food Science Institute. Institute of Biomedical Engineering. School of Food and Life Sciences, College of Biomedical Science and Engineering, Inje University) ;
  • Jin, Kyong-Suk (Food Science Institute, Institute of Biomedical Engineering, School of Food and Life Sciences, College of Biomedical Science and Engineering, Inje University) ;
  • Kim, Young-Hun (Food Science Institute, Institute of Biomedical Engineering, School of Food and Life Sciences, College of Biomedical Science and Engineering, Inje University) ;
  • Jun, Mi-Ra (Division of Food Science, Dong-A University) ;
  • Lim, Ho-Jin (Department of Environmental Engineering, Kyungpook National University) ;
  • Jo, Wan-Kuen (Department of Environmental Engineering, Kyungpook National University) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Jeong, Woo-Sik (Food Science Institute, Institute of Biomedical Engineering, School of Food and Life Sciences, College of Biomedical Science and Engineering, Inje University)
  • Published : 2008.04.30
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Abstract

Cellular protection against carcinogens could be achieved by the induction of phase 2 detoxifying and antioxidant enzymes such as glutathione S-transferase (GST), NAD(P)H:quinone oxidoreductase 1 (NQO1) and heme oxygenase 1 (HO1). Nuclear transcription factor E2-related factor 2 (Nrf2) binds to antioxidant response element (ARE) in the promoter region of these genes and the resulting transactivation occurs. In the present study the effect of gujeolcho (Chrysanthemum zawadskii) roots on the Nrf2-ARE pathway were investigated. C. zawadskii root extract was fractionated with a series of organic solvents and their ability to induce Nrf2-ARE pathway was examined. We separated the most potent dichloromethane (DCM) fraction into 12 sub-fractions and found several sub-fractions with strong effects on the Nrf2-ARE pathway. Fraction 4 strongly induced the ARE-reporter gene activity as well as Nrf2 expression. Sitosterol was isolated as a major compound in fraction 4 although its activity was not as potent as its mother fraction. These results indicate that C. zawadskii roots might be used as a potential natural chemopreventive source.

Keywords

References

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