Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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SPR-based Antibody-Antigen Interaction for Real Time Analysis of Carbamate Pesticide Residues

  • Yang, Gil-Mo (Department of Biological Engineering, University of Missouri-Columbia) ;
  • Kang, Suk-Won (National Institute of Agricultural Engineering, Rural Development Administration)
  • Published : 2008.02.29
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Abstract

This research was conducted to develop a quick and sensitive method of detecting carbamate residues using the immobilization of antibody-antigen interactions with surface plasmon resonance (SPR). We have used commercialized surface plasmon resonance equipment (Biacore 3000). The antibody used for the immunoassay was specific for glutathione-s-transferase (GST) and the antigens included several carbamate pesticides (carbofuran, carbaryl, and benfuracarb). When antigens were applied to the protein GST, the detection limit was 2 ng/mL of carbamate pesticide. The fabricated protein GST maintained its activity for over 200 measurements. Thus we determined that the SPR biosensors could detect the specific reversible binding of a reactant in solution to a binding partner immobilized on the surface of the sensor and allow real-time detection and monitoring.

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