대한한방내과학회지 (The Journal of Internal Korean Medicine)
- 제29권1호
- /
- Pages.42-66
- /
- 2008
- /
- 1226-9174(pISSN)
부자의 메탄올 추출물에 의한 신세포암의 증식억제 및 세포사멸 유도
Inhibition of Cell Proliferation and Induction of Apoptosis by Methanolic Extract of Aconiti Tuber in Human Renal Cell Carcinoma Cells
- Kim, Jong-Hwan (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University) ;
- Choi, Yung-Hyun (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University) ;
- Hwang, Won-Deuk (Department of Oriental Medicine, College of Oriental Medicine, Dongeui University)
- 발행 : 2008.03.31
초록
Aconiti Tuber is a traditional medicinal plant generally used in Oriental medicine therapy. In this study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of Aconiti tuber (MEBJ) in Caki-1 human renal cell carcinoma cells. It was found that MEBJ could inhibit, in a dose-dependent manner, cell growth which was associated with apoptotic cell death such as formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase. Apoptosis of Caki-1 cells by MEBJ was associated with an up-regulation of pro-apoptotic Bax expression, and a down-regulation of anti-apoptotic Bcl-2 in a dose-dependent manner; however, the levels of IAP family were not affected. MEBJ treatment also induced the proteolytic activation of caspase-3 and -8, and a inhibition of poly(ADP-ribose) polymerase (PARP) and