Chemical Composition and Antimicrobial Activity of Essential Oil from Cones of Pinus koraiensis

  • Lee, Jeong-Ho (Korea National Aboretum) ;
  • Yang, Hye-Young (Department of Biological Science and Institute of Bioscience and Biotechnology, Myongji University) ;
  • Lee, Hong-Sub (Ildong Pharmaceutical Co., Ltd.) ;
  • Hong, Soon-Kwang (Department of Biological Science and Institute of Bioscience and Biotechnology, Myongji University)
  • Published : 2008.03.31

Abstract

The essential oil from the cones of Pinus koraiensis was prepared after removing the seeds, and its chemical composition analyzed using gas chromatography-mass spectrometry (GC-MS). Hydrodistillation of the P. koraiensis cones yielded 1.07% (v/w) of essential oil, which was almost three times the amount of essential oil extracted from the needles of the same plant. Moreover, the antimicrobial activities of the oil against the growth of Gram-positive bacteria, Gram-negative bacteria, and fungi were evaluated using the agar disc diffusion method and broth microdilution method. Eighty-seven components, comprising about 96.8% of the total oil, were identified. The most abundant oil components were limonene (27.90%), ${\alpha}$-pinene (23.89%), ${\beta}$-pinene (12.02%), 3-carene(4.95%), ${\beta}$-myrcene (4.53%), isolongifolene (3.35%), (-)-bornyl acetate (2.02%), caryophyllene (1.71%), and camphene (1.54%). The essential oil was confirmed to have significant antimicrobial activities, especially against pathogenic fungal strains such as Candida glabrata YFCC 062 and Cryptococcus neoformans B 42419. Therefore, the present results indicate that the essential oil from the cones of Pinus koraiensis can be used in various ways as a nontoxic and environmentally friendly disinfectant.

Keywords

References

  1. Asset, G., B. Staels, R. L. Wolff, E. Bauge, Z. Madj, J. C. Fruchart, and J. Dallongeville. 1999. Effects of Pinus pinaster and Pinus koraiensis seed oil supplementation on lipoprotein metabolism in the rat. Lipids 34: 39-44 https://doi.org/10.1007/s11745-999-335-2
  2. Bagchi, D., M. Bagchi, S. J. Stohs, D. K. Das, S. D. Ray, C. A. Kuszynski, S. S. Joshi, and H. G. Pruess. 2000. Free radicals and grape seed proanthocyanidin extract: Importance in human health and disease prevention. Toxicology 148: 187-197 https://doi.org/10.1016/S0300-483X(00)00210-9
  3. Cha, J.-D., E.-K. Jung, B.-S. Kil, and K.-Y. Lee. 2007. Chemical composition and antibacterial activity of essential oil from Artemisia feddei. J. Microbiol. Biotechnol. 17: 2061-2065
  4. Chung, K.-H., K.-S. Yang, J. Kim, J.-C. Kim, and K.-Y. Lee. 2007. Antibacterial activity of essential oils on the growth of Staphylococcus aureus and measurement of their binding interaction using optical biosensor. J. Microbiol. Biotechnol. 17: 1848-1855
  5. Cowan, M. M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12: 564-582
  6. Cox, S. D., C. M. Mann, J. L. Markham, H. C. Bell, J. E. Gustafson, J. R. Warmington, and S. G. Wyllie. 2000. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J. Appl. Microbiol. 88: 170-175 https://doi.org/10.1046/j.1365-2672.2000.00943.x
  7. Cox, S. D., J. E. Gustafson, C. M. Mann, J. L. Markham, Y. C. Liew, R. P. Hartland, H. C. Bell, J. R. Warmington, and S. G. Wyllie. 1998. Tea tree oil causes K+ leakage and inhibits respiration in Escherichia coli. Lett. Appl. Microbiol. 26: 355-358 https://doi.org/10.1046/j.1472-765X.1998.00348.x
  8. Deans, S. G. and G. Ritchie. 1987. Antibacterial properties of plant essential oils. Int. J. Food Microbiol. 5: 165-180 https://doi.org/10.1016/0168-1605(87)90034-1
  9. Gustafson, J. E., Y. C. Liew, S. Chew, J. L. Markham, H. C. Bell, S. G. Wyllie, and J. R. Warmington. 1998. Effects of tea tree oil on Escherichia coli. Lett. Appl. Microbiol. 26: 194-198 https://doi.org/10.1046/j.1472-765X.1998.00317.x
  10. Harkenthal, M., J. Reichling, H. K. Geiss, and R. Saller. 1999. Comparative study on the in vitro antibacterial activity of Australian tea tree oil, cajuput oil, niaouli oil, manuka oil, kanuka oil, and eucalyptus oil. Pharmazie 54: 460-463
  11. Helander, I. M., H.-L. Alakomi, K. Latva-Kala, T. Mattila-Sandholm, I. Pol, E. J. Smid, L. G. M. Gorris, and A. Von Wright. 1998. Characterization of the action of selected essential oil components on Gram-negative bacteria. J. Agric. Food Chem. 46: 3590-3595 https://doi.org/10.1021/jf980154m
  12. Hong, E.-J., A.-J. Na, B.-G. Choi, C.-C. Choi, and E.-B. Jeung. 2004. Antibacterial and antifungal effects of essential oils from coniferous trees. Biol. Pharm. Bull. 27: 863-866 https://doi.org/10.1248/bpb.27.863
  13. Lee, S. O., I.-K. Park, G. J. Choi, H. K. Lim, K. S. Jang, K. Y. Cho, S.-C. Shin, and J.-C. Kim. 2007. Fumigant activity of essential oils and components of Illicium verum and Schizonepeta tenuifolia against Botrytis cinerea and Colletotrichum gloeosporioides. J. Microbiol. Biotechnol. 17: 1568-1572
  14. Li, K., L. Qingwang, L. Jian, T. Zhang, Z. Han, D. Gao, and F. Zheng. 2007. Antitumor activity of the procyanidins from Pinus koraiensis bark on mice bearing U14 cervical cancer. Yakugaku Zasshi 127: 1145-1151 https://doi.org/10.1248/yakushi.127.1145
  15. Porter, N. G. and A. L. Wilkins. 1999. Chemical, physical and antimicrobial properties of essential oils of Leptospermum scoparium and Kunzea ericoides. Phytochem. Oxford 50: 407-415 https://doi.org/10.1016/S0031-9422(98)00548-2
  16. Saller, R., T. Berger, J. Reichling, and M. Harkenthal. 1998. Pharmaceutical and medicinal aspects of Australian tea tree oil. Phytomedicine 5: 489-495 https://doi.org/10.1016/S0944-7113(98)80048-2
  17. Williams, L. R. and V. Home. 1995. A comparative study of some essential oils for potential use in topical applications for the treatment of the yeast Candida albicans. Aust. J. Med. Herbalism 7: 57-62
  18. Zhang, C., H. Li, T. Yun, Y. Fu, C. Liu, B. Gong, and B. Neng. 2008. Chemical composition, antimicrobial and antioxidant activities of the essential oil of Tibetan herbal medicine Dracocephalum heterophyllum Benth. Nat. Prod. Res. 22: 1-11 https://doi.org/10.1080/14786410701619076