Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Microbial Biodegradation and Toxicity of Vinclozolin and its Toxic Metabolite 3,5-Dichloroaniline

  • Lee, Jung-Bok (School of Bioresource Sciences, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Kim, Jong-Sik (Department of Biological Science, Andong National University) ;
  • Jo, Min-Sub (School of Bioresource Sciences, Andong National University) ;
  • Jeon, Chun-Pyo (School of Bioresource Sciences, Andong National University) ;
  • Jang, Jong-Ok (School of Bioresource Sciences, Andong National University) ;
  • Kim, Jang-Eok (Department of Agricultural Chemistry, Kyungpook National University) ;
  • Kwon, Gi-Seok (School of Bioresource Sciences, Andong National University)
  • Published : 2008.02.29
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Abstract

Vinclozolin, an endocrine disrupting chemical, is a chlorinated fungicide widely used to control fungal diseases. However, its metabolite 3,5-dichloroaniline is more toxic and persistent than the parent vinclozolin. For the biodegradation of vinclozolin, vinclozolin- and/or 3,5-dichloroaniline-degrading bacteria were isolated from pesticide-polluted agriculture soil. Among the isolated bacteria, a Rhodococcus sp. was identified from a 16S rDNA sequence analysis and named Rhodococcus sp. T1-1. The degradation ratios for vinclozolin or 3,5-dichloroaniline in a minimal medium containing vinclozolin $(200{\mu}ml)$ or 3,5-dichloroaniline $(120{\mu}g/ml)$ were 90% and 84.1%, respectively. Moreover, Rhodococcus sp. T1-1 also showed an effective capability to biodegrade dichloroaniline isomers on enrichment cultures in which they were contained. Therefore, these results suggest that Rhodococcus sp. T1-1 can bioremediate vinclozolin as well as 3,5-dichloroaniline.

Keywords

References

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