Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antibacterial Activity of HTI Isolated from Oriental Medicine, Hyungbangjihwang-tang

형방지황탕으로부터 분리된 HTI의 항균 활성

  • Sung, Woo-Sang (Departmentn of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Seu, Young-Bae (Departmentn of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong-Gun (Departmentn of Microbiology, College of Natural Sciences, Kyungpook National University)
  • Published : 2008.03.28
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Abstract

Hyungbangjihwang-Tang (HT), an Oriental herbal formula, has been known to play a role which helps to recover vigor of human in the Orient. In this study, antibacterial substance (HTI) was purified from the ethyl-acetate extracts of HT by using $SiO_2$ column chromatography and HPLC, and the antibacterial effects of HTI were investigated. By using the CLSI broth micro-dilution assay, the activity of HTI was evaluated against human pathogenic Gram-positive and Gram-negative bacterial strains including the clinical isolates of methicillin-resistant Staphylococcus aureus. The results demonstrated that HTI showed broad spectrum antibacterial activities against all bacterial strains tested. In conclusion, HTI is an interesting new molecule for its potential in anti-infective drug discovery and for future studies on activity-structure relationship through analysis of its chemical structure.

형방지황탕은 동양 고유의 약처방으로 사람의 기력 회복에 도움을 주는 것으로 알려져 있다. 본 연구에서는 $SiO_2$ 컬럼 크로마토그래피와 HPLC를 이용하여 형방지황탕 유래의 항균 물질 (HTI)을 분리하여 그의 항균 활성을 측정하였다. 인체 감염성 그램-양성균, 그램-음성균 및 환자에게서 분리된 메티실린-내성 황색 포도상구균을 대상으로하여 CLSI방법을 이용하여 HTI 항균 활성을 측정하였다. 그 결과, HTI은 실험한 모든 세균에 대하여 넓은 범위의 항균 활성을 나타냄을 확인할 수 있었다. 이 결과를 바탕으로 하여 HTI이 항균제로서의 발전 가능성을 가진 신물질임을 확인하였으며, HTI의 화학 구조 결정을 통하여 구조와 활성간의 연구를 시행할 것이다.

Keywords

References

  1. Bailie, G. R. and D. Neal. 1988. Vancomycin ototoxicity and nephrotoxicity: A review. Med. Toxicol. Adverse Drug Exp. 3: 376-386 https://doi.org/10.1007/BF03259891
  2. Brumfitt, W. and J. Hamilton-Miller. 1989. Methicillin-resistant Staphylococcus aureus. N. Engl. J. Med. 320: 1188-1196 https://doi.org/10.1056/NEJM198905043201806
  3. Cha, D. S. and M. S. Chinnan. 2004. Biopolymer-based antimicrobial packaging: A Review. Crit. Rev. Food Sci. Nutr. 44: 223-237 https://doi.org/10.1080/10408690490464276
  4. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing, fifteenth informational supplement, approved standard MS100- S15, CLSI, Wayne, Pa
  5. Cohen, M. L. 1992. Epidemiology of drug resistance; implications for a post-antimicrobial era. Science 257: 1050-1055 https://doi.org/10.1126/science.257.5073.1050
  6. Jeong, J. H., E. -H. Seo, S. J. Park, D. -M. Goo, B. -M. Lim, Y. -J. Kim, H. -G. Kang, and Y. -B. Seu. 2004. Isolation of antimicrobial compound against Klebsiella pneumonia from Hyeongbangjiwhangtang. Kor. J. Herbology 19: 127-135
  7. Jia, W. 2004. The rediscovery of ancient Chinese herbal formulas. Phytother. Res. 18: 681-686 https://doi.org/10.1002/ptr.1506
  8. Mulligan, M. E., K. A. Murray-Leisure, H. C. Standiford, J. F. John, J. A. Korvick, C. A. Kauffman, and V. L. Yu. 1993. Methicillin-resistant Staphylococcus aureus: a consensus review of the microbiology, pathogenesis, and epidemiology with implication for prevention and management. Am. J. Med. 94: 313-328 https://doi.org/10.1016/0002-9343(93)90063-U
  9. Park, Y., D. G. Lee, S. -H. Jang, E. -R. Woo, H. G. Jeong, C. - H. Choi, and K. -S. Hahm. 2003. A Leu-Lys-rich antimicrobial peptide: activity and mechanism. Biochim. Biophys. Acta 1645: 172-182 https://doi.org/10.1016/S1570-9639(02)00541-1
  10. Schouten, M. A., J. A. A. Hoogkamp-Korstanje, J. F. G. Meis, A. Voss, and the European VRE Study Group. 2000. Prevalence of vancomycin-resistant enterococci in Europe. Eur. J. Clin. Microbiol. Infect. Dis. 19: 816-822 https://doi.org/10.1007/s100960000390
  11. Townsend, D. E., N. Ashdown, S. Bolton, J. Bradley, G. Duckworth, E. C. Moorhouse, and W. B. Grubb. 1987. The international spread of methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 9: 60-71 https://doi.org/10.1016/0195-6701(87)90097-1