Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Construction of A Bacteriocidal Yeast Producing Bacteriocin OR-7

박테리오신 OR-7을 생산하는 항균 효모의 제작

  • Lee, Ok-Hee (Department of Bioscience and Biotechnology, Silla University) ;
  • Jang, Min-Kyung (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience and Biotechnology, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Silla University) ;
  • Ha, Bae-Jin (Department of Bioscience and Biotechnology, Silla University) ;
  • Ahn, Ik-Yong (Youngnam Flour Mills Co., LTD.) ;
  • Cho, Dong-In (Youngnam Flour Mills Co., LTD.) ;
  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Silla University)
  • Published : 2008.06.28
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Abstract

In order to obtain yeast cells producing a bacteriocin OR-7, the 180 bp polynucleotide corresponding to the OR-7 gene including codons for start and stop was chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Campylobacter jeuni, Escherichia coli and Pseudomonas aeruginosa. This result indicates that yeast cells producing OR-7 possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative C. jejuni, E. coli and P. aeruginosa cells. The recombinant yeast strain constructed in this study can be applied in the food preservative or animal feed.

박테리오신의 일종인 OR-7을 생산하는 효모의 제작을 위하여 180 bp 길이의 개시코돈과 종지코돈을 포함하는 OR-7의 유전자를 합성하여 효모 발현 vector pAUR123에 클로닝하여 재조합 DNA를 작성하였다. 재조합 DNA로 형질전환된 효모가 박테리오신 OR-7 생산유전자를 가지고 있음을 효모로부터 분리된 플라스미드를 이용한 PCR로 확인하였고, OR-7의 생산은 SDS-PAGE로 확인하였다. 형질전환 효모는 그람양성 대표세균인 고초균(B. subtiliws)과 그람음성 장내세균인 대장균(E. coli)에 대해 항균활성을 나타냈다. 또한, 농흉이나 중이염의 원인이 되는 녹농균(P. aeruginosa)과 식중독균(C. jejuni)에 대해서도 항균활성을 나타냈다. 이 연구의 결과로 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질로 사용할 수 있는 박테리오신을 산업적으로 생산할 수 있는 효모세포를 제작하였다.

Keywords

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