Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Aqueous Extract from Asiasari Radix on ${\alpha}$-melanocyte Stimulating Hormone Induced Melanogenesis in B16F10 Melanoma Cells

세신의 열수추출물이 ${\alpha}$-melanocyte Stimulating Hormone에 의해 유도된 B16F10 세포의 멜라닌 생성에 미치는 영향

  • Lee, Jun-Hyuk (Chemistry and Biotechnology Examinations Bureau, Korean Intellectual Property Office) ;
  • Shin, Dong-Yeok (Departments of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Choi, Yung-Hyun (Departments of Biochemistry, College of Oriental Medicine, Dong-Eui University) ;
  • Chung, Kyung-Tae (Departments of Biomedical Laboratory Science, College of Natural Science, Dong-Eui University) ;
  • Kang, Byoung-Won (Departments of Chemistry, College of Natural Science, Dong-Eui University) ;
  • Jeong, Seong-Yun (Korea BIO-IT Foundry Center) ;
  • Choi, Byung-Tae (School of Oriental Medicine, Pusan National University)
  • 이준혁 (특허청 화학생명공학심사국) ;
  • 신동역 (동의대학교 한의과대학 생화학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 정경태 (동의대학교 자연과학대학 임상병리학과) ;
  • 강병원 (동의대학교 자연과학대학 화학과) ;
  • 정성윤 (부산대학교 BIT융합기술산업화지원기반구축사업단) ;
  • 최병태 (한의학전문대학원)
  • Published : 2008.06.25
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Abstract

The aqueous extract from Asiasari radix (AEAR) was used to investigate the effect of ${\alpha}$-melanocyte stimulating hormone induced melanogenesis in B16F10 mouse melnoma cells. The treatment with AEAR at the 1.0 and 2.0 mg/ml level significantly inhibited the biosynthesis of melanin without changes of cell growth and morphology compared with untreated control. The AEAR-treated cells at the 2.0 mg/ml level were more efficient than commercial arbutin at 0.1 mg/ml. The tyrosinase activity also significantly decreased in AEAR-treated cells at the 1.0 and 2.0 mg/ml level. The Western analyses confirmed the slightly decreased expression of tyrosinase by AEAR treatment. These results indicate that AEAR may contribute to the inhibition of melanin biosynthesis through regulating tyrosinase activity and expression and serve as a new candidate in the design of new skin-whitening or therapeutic agents.

Keywords

References

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