Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-inflammatory Effect of Cinnamomi Ramulus on Collagen Induced Arthritis;a Model for Rheumatoid Arthritis in DBA/1J Mice and Cytokine Production in Raw264.7 Cells

계지(桂枝)의 collagen 유발 관절염에 대한 소염 효과;DBA/1J mouse에서의 병태 관찰 및 RAW264.7에서의 cytokine 분비측정

  • Kim, Sung-Joon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University Institute of Oriental Medicine, Kyunghee University) ;
  • Park, Sung-Min (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Brain Korea 21 Oriental Medical Science Center) ;
  • Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Cancer Preventive Material Development Research Center) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University Institute of Oriental Medicine, Kyunghee University)
  • 김성준 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 박성민 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 강희 (BK21 경희대학교 한의과학사업단) ;
  • 심범상 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 김성훈 (암예방소재개발연구센터) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실 경희대학교 한의학연구소)
  • Published : 2008.06.25
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Abstract

In order to examine anti-inflammatory effect of Cinnamomi Ramulus (CR) alcohol extract on rheumatoid arthritis, the present study investigated the viability and TNF-${\alpha}$ production in Raw264.7 cells treated with CR and collagen induced arthritis in DBA/1J mice which were orally administered with CR prior to immunization. The results are as follows: CR extract at $20{\mu}g/ml$ inhibited the viability of Raw264.7 by 50%. CR at 15 and $20{\mu}g/ml$ showed a significant decrease in TNF-${\alpha}$ levels from Raw264.7 cells treated with LPS. CR administration decreased arthritic index in DBA/1J mice immunized with bovine collagen type II but it did not reach statistical significance. CR administration significantly decreased spleen weights obtained from mice in 6 weeks after immunization. CR administration significantly decreased serum anti-type II collagen antibody levels compared with control group. CR administration decreased serum IL-6 levels compared with control group but it did not reach statistical significance.

Keywords

References

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