Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Antiviral Activity of Zanthoxylum Species against Influenza Virus

인플루엔자 바이러스에 대한 Zanthoxylum속의 항바이러스 효과 검정

  • Choi, Hwa-Jung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Song, Jae-Hyoung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Kwon, Dur-Han (Korea Research Institute of Bioscience and Biotechnology) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Ahn, Young-Joon (School of Agricultural Biotechnology, Seoul National University)
  • 최화정 (한국생명공학연구원 천연물의약연구센터) ;
  • 송재형 (원광대학교 한의학전문대학원) ;
  • 권두한 (한국생명공학연구원 천연물의약연구센터) ;
  • 백승화 (원광대학교 한의학전문대학원) ;
  • 안용준 (서울대학교 농생명과학대학)
  • Published : 2008.08.30
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Abstract

We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10${\mu}g/m{\ell}$ with no citotoxicity (50${\mu}g/m{\ell}$) against all of three viruses. In addition, only oseltamivir showed antiviral activity with $IC_{50}$ of 65.3${\mu}g/m{\ell}$ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (-1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.

Keywords

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