Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Gamma Irradiation Induces a Caspase-dependent Apoptotic Mechanism in Human Prostate Cancer PC-3 Cells

인간 남성호르몬 비의존형 전립선 PC-3 암세포에서 감마선의 Caspase-의존성 세포자멸사 유도 효과

  • Chang, Jeong-Hyun (Department of Clinical Laboratory Science, College of Health & Therapy, Daegu Hanny University) ;
  • Kim, Dong-Hyun (Department of Biomedical Engineering, School of Medicine, Pusan National University) ;
  • Jeon, Gye-Rok (Department of Biomedical Engineering, School of Medicine, Pusan National University) ;
  • Kwon, Heun-Young (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • 장정현 (대구한의대학교 보건치료대학 임상병리학과) ;
  • 김동현 (부산대학교 의과대학 의공학협동과정) ;
  • 전계록 (부산대학교 의과대학 의공학협동과정) ;
  • 권헌영 (부산가톨릭대학교 보건과학대학 임상병리학과)
  • Published : 2008.08.30
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Abstract

Prostate cancer is the most predominant cancer in men and related death rate increases every year. Till date, there is no effective therapy for androgen independent prostate cancer. To investigate the mechanism for cell growth inhibition or apoptosis in human androgen independent prostate cancer PC-3 cells after gamma irradiation. The aim of this study was to examine the potential of gamma irradiation to induce apoptosis in PC-3 cells and to assess the mechanism of gamma irradiation-induced apoptosis. Five different assays were employed in this study: cell proliferation assay, morphological assessments of apoptotic cells, DNA fragmentation analysis, quantification of apoptosis by annexin V (AV) and propidium iodide (PI) staning, and western blot analysis. Cell viability was inversely related to radiation dose. DAPI-positive cells were detected 48 hr after 40 Gy radiation exposure. And nuclear morphological changes of cells were observed by gamma irradiation. DNA ladder patterns in the cells exposed to gamma-radiation were appeared at 24 hr. Also, gamma irradiation induces apoptosis of PC-3 cells via Caspase3, Bax and PARP-dependent fashion.

전립선 암은 현대 남성에게 걸리기 쉬운 질환으로 매년 점점 증가하는 암 사망률 중 하나이다. 하지만 남성호르몬 비의존형 전립선 암 치료에 대한 효과가 거의 없어 이에 본 연구에서는 남성호르몬 비의존형 전립선 암세포인 PC-3 세포에서 감마선이 세포 성장 억제와 세포자멸사 기작에 대해 알아보고자 한다. 그리하여 본 연구에서는 5가지 방법으로 즉, 세포증식 억제, apoptotic cell의 형태학적 변화, DNA 분절 분석, AV/PI 염색, western blot 분석법을 사용하여 수행하였다. 본 연구의 결과로 감마선을 처리한 PC-3세포에서 형태학적(분절화) 변화와 DNA ladder가 관찰되었다. 또한 감마선을 처리한PC-3세포에서는 apoptosis와 관련된 Caspase3와 PARP cleavage가 유발되었고 Bax 단백질 증가도 보였다.

Keywords

References

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