Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Water Extract of Deer Antler in Osteoclast Differentiation

녹용 물 추출물의 파골세포 분화 억제효과

  • Kwak, Han-Bok (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute) ;
  • Kim, Ju-Ho (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute) ;
  • Kim, Dong-Joo (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute) ;
  • Kwon, Young-Mi (Department of Diagnostic Radiology, Oriental Medicine, Wonkwang University) ;
  • Oh, Jae-Min (Department of Anatomy, School of Medicine, and Wonkwang Medical Science Institute) ;
  • Kim, Yun-Kyung (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute)
  • 곽한복 (원광대학교 의과대학 해부학교실 의과학 연구소) ;
  • 김주호 (원광대학교 약학대학 한약학과 원광한약연구소) ;
  • 김동주 (원광대학교 의과대학 해부학교실 의과학 연구소) ;
  • 권영미 (원광대학교 한의과대학 진단 방사선학과) ;
  • 오재민 (원광대학교 의과대학 해부학교실 의과학 연구소) ;
  • 김윤경 (원광대학교 약학대학 한약학과 원광한약연구소)
  • Published : 2008.08.25
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Abstract

Natural substances have recently received much attention as therapeutic drugs to prevent many diseases in humans because they avoid the many side effects of treatment with chemical compounds. We examined the effect of water extract of deer antler in RANKL-induced osteoclast differentiation. The effects of water extract of deer antler in osteoclast differentiation were determined by culture of bone marrow macrophages (BMMs). The mRNA expression levels of c-Fos, NFATc1, TRAP, and GAPDH in BMMs were analyzed by RT-PCR. Cell lysates were obtained from the treated cells, the expression levels of c-Fos and NFATc1 were determined by western blotting with antibodies for c-Fos and NFATc1. Water extract of deer antler greatly inhibited RANKL-mediated osteoclast differentiation in osteoclast precursors without cytotoxicity. Water extract of deer antler inhibited the expression of c-Fos and NFATc1 in BMMs treated with RANKL. Our findings suggest that water extract of deer antler inhibited osteoclast differentiation by suppressing c-Fos and NFATc1 expression in response to RANKL. These results demonstrate that water extract of deer antler may be a useful the treatment of bone-related disease such as osteoporosis.

Keywords

References

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