Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats

흰쥐에서 고농도 산소 흡입에 의한 급성 폐손상 시 호중구성 산화성 스트레스의 역할

  • Moon, Yongsuck (Department of Anatomy, Catholic University of Daegu, School of Medicine) ;
  • Kim, Jihye (Department of Physiology, Catholic University of Daegu, School of Medicine) ;
  • Lee, Young Man (Department of Physiology, Catholic University of Daegu, School of Medicine)
  • 문용석 (대구가톨릭대학교 의과대학교 해부학교실) ;
  • 김지혜 (대구가톨릭대학교 의과대학교 생리학교실) ;
  • 이영만 (대구가톨릭대학교 의과대학교 생리학교실)
  • Received : 2008.09.23
  • Accepted : 2008.11.10
  • Published : 2008.12.30
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Abstract

Background: This study examined the role of neutrophilc oxidative stress in an $O_2-induced$ acute lung injury (ALI). Methods: For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage. Forty-eight hours after $O_2$ breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed Results: Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The $O_2-induced$ neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase $A_2$ ($cPLA_2$) activity in the lung, histological changes and BAL cytospin morphology. Conclusion: In part, ALI-caused by oxygen is affected by neutrophils especially by the generation of free radicals.

연구배경: 산소독에 의한 급성 폐손상에 대한 기전은 아직 확실히 알려져 있지 않다. 급성호흡곤란증후군에서 보이는 호중구의 산소기 생성에 따른 조직의 손상이 산소독에 의한 손상에서도 관여하는지를 확인하고자 하였다. 방 법: Plastic cage 내의 기압을 1기압으로 고정하고 흰쥐에게 순수한 산소를 48시간 호흡시킨 후 호중구가 폐장 내로 침윤함으로써 나타나는 급성 폐손상을 생화학적인 지표 및 형태학적인 관찰 등을 통하여 검사하였다. 결 과: 흰쥐에게 순수한 산소를 48시간 호흡하게 한 경우 폐부종, 호중구의 침윤, 폐장 내 MDA 및 $cPLA_2$ 활동도의 증가가 관찰되었고, 형태학적으로도 탐식구 특히 호중구의 침윤에 따른 폐장의 손상이 관찰되었다. 결 론: 산소독에 의한 급성폐손상의 기전은 고농도의 산소에 의한 산소기 생성이 그 원인으로 생각 되지만 산소기의 작용기전은 부분적으로 $cPLA_2$활성도 증가에 의한 호중구의 조직 내 침윤에 따른 이차적 산소기 형성이 그 원인으로 생각되며 시간이 경과할수록 호중구에 폐장 내 침윤에 따른 손상이 더 심해질 것으로 생각된다.

Keywords

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