Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Detection of Marine Birnavirus(MABV) from Marine Fish in the Southern Coast of Korea

남해안 자연산 어류에서 Marine birnavirus(MABV)의 검출

  • Yun, Hyun-Mi (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Kim, Seok-Ryel (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Lee, Wol-La (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Jung, Sung-Ju (Division of Food Science and Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Division of Food Science and Aqualife Medicine, Chonnam National University)
  • 윤현미 (전남대학교 식품.수산생명의학부) ;
  • 김석렬 (전남대학교 식품.수산생명의학부) ;
  • 이월라 (전남대학교 식품.수산생명의학부) ;
  • 정성주 (전남대학교 식품.수산생명의학부) ;
  • 오명주 (전남대학교 식품.수산생명의학부)
  • Published : 2008.02.25
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Abstract

Marine birnavirus(MABV) are well known fish pathogens in Asian countries such as Korea, Japan, and China. Prevalence of viral disease, geological distribution and host or reservoir of viruses were investigated from wild marine fishes in southern coast of Korea in 2003 and 2005. RT-PCR results showed that MABV were detected in 17 fishes(10.6%) from 160 fishes. Comparative analysis of the nucleotide sequences and the deduced amino acids of MABV genome from wild fishes were similar to reference strains of MABV and distinguished with IPNV strains.

Marine birnavirus(MABV)는 중요한 어류 병원체로서 일본 양식산 방어서 처음 분리되어 이후 다양한 해산어종에서 MABV 분리가 보고되었으며 환경적인 샘플인 저질, 해양의 동물성 플랑크톤과 해수에서도 MABV 유전자가 검출되고 있다. 본 연구는 Marine birnavirus disease 에 대한 예방학적 접근의 일환으로서 자연산 어류로부터의 MABV 검출 및 지리적 분포, 보균 어종에 대한 유전학적인 조사를 목적으로 2003년과 2005년도에 동중국해역 3지점, 서해안 5지점 그리고 남해안 5지점에서 어류를 채집하였다. RT-PCR(Reverse transcriptase-Polymerase Chain Reaction)을 이용한 연구결과 자연산 해산어류 160마리 중 13종 17마리에서 MABV가 거출되어 감염율10.6%를 보였다. 조사된 13종의 어류 중 특히 농어목에서 가장 높는 검출율을 보였다. 자연산 어류에서 분리한 MABV 분리균주는 염기서열 분석에서 94.7%-100%, 아미노산 분석에서 97.2-100% 유사성을 나타내었으며 IPNV strain과는 구분되며 MABV에 속하였다.

Keywords

References

  1. Chou, H. Y., H. J. Li, C. F. Lo, 1994. Pathogenicity of a birnavirus to hard clam (Meretrix Iusoria) and effect of temperature stress on its virulence. Fish Pathol. 29, 171−175
  2. Dobos, P., 1995. The molecular biology of infectious pancreatic necrosis virus (IPNV). Annu. Rcv. Fish Dis. 5, 25−54
  3. Heppell, J., L. Berthiaume, F. Corbin, E. Tarrab, J. Lecomte and M. Arella, 1993. Comparison of amino acid sequences deduced from a cDNA fragment obtained from infectious pancreatic necrosis virus (IPNV) strains of different serotypes. Virology, 195, 840−844
  4. Hosono, N., S. Suzuki and R. Kusuda, 1996. Genogrouping of bir-naviruses isolated from marine fish, a comparison of VP2/NS junction regions on genome segment A. J. Fish Dis., 19, 295−302
  5. Iida, Y., K. Masumura, T. Nakai, M. Sorimachi, T. Matsuda, 1989. A viral disease in larvae and juveniles of the Japanese flounder Paralichthys olivaceus. J. Aquat. Anim. Health, 1, 7−12
  6. Isshiki, T., K. Kawai and R. Kusuda, 1989. Incidence of yellowtail ascites virus (YAV) in wild yellowtail fingerling. Nippon Suisan Gakkaishi., 55, 633−637
  7. Isshiki, T., T. Nagano, K. Kanehira., S. Suzuki, 2004. Distribution of marine birnavirus in cultured marine fish species from Kagawa Prefecture, Japan. J. Fish Dis., 27, 89−98
  8. Kamakura, M., S. Suzuki and R. Kusuda, 1995. Characterization of birnavirus isolated from diseased tiger puffer. Program and Abstracts of the Autumn Meeting of the Japanese Society of Fish Pathology, Mie, Japan, September 1995, p. 14
  9. Kitamura, S. I. and S. Suzuki, 2000. Occurrence of marine birnavirus through the year in coastal seawater in the Uwa sea. Mar. Biotechnol., 2, 188−194
  10. Kitamura, S. I., S. J. Jung and S. Suzuki, 2000a. Seasonal change of infective state of marine birnavirus in Japanese pearl oyster Pinctada fucata. Arch. Virol., 145, 2003−2014
  11. Kitamura, S. I., Y. Tomaru, Z. Kawabata and S. Suzuki, 2000b. Detection of marine birnavirus in the Japanese pearl oyster Pinctada fucata and seawater from different depths. Dis. Aquat. Org., 50, 211−217
  12. Kitamura, S. I., S. I. Kamata, S. I Nakano and S. Suzuki, 2003. Detection of marine birnavirus genome in zooplankton collested from the Uwa Sea, Japan. Dis. Aquat. Org., 54, 69−72
  13. Sohn, S. G., M. Park, J. Do, J. Choi and J. Park, 1995. Birnavirus isolated from cultured flounder in Korea. Fish Pathol., 30, 279−280
  14. Sorimachi M. and T. Hara, 1985. Characteristics and pathogenicity of a virus isolated from yellowtail fingerlings showing ascites. Fish Pathol., 19, 231−238. (in Japanese)
  15. Suzuki, S. and M. Nojima, 2000. Distribution of a marine birnavirus in wild molluscan shellfish species from Japan. Fish Pathol., 34, 121−126
  16. Suzuki, S., N. Hosono, R. Kusuda, 1997. Detection of aquatic birnavirus gene from marine fish using a combination of reverse transcription and nested PCR. J. Mar. Biotechnol., 5, 205−209
  17. Suzuki, S., M. Kamakura, R. Kusuda, 1998. Isolation of birnavirus from Japanese pearl oyster Pinctada fucata. Fish Sci., 64, 342−343
  18. Takano, R., K. Mori, T. Nishizawa, M. Arimoto and K. Muroga, 2001. Isolation of viruses from wild Japanese flounder Paralichthys olivaceus. Fish Pathol., 36, 153−160
  19. Watanabe, L., R. Pakingking, Jr., H. Iida, T. Nishizawa, Y. Iida, M. Arimoto and K. Muroga, 2002. Isolation of aquabirnavirus and viral hemorrhagic septicemia virus (VHSV) from wild marine fishes. Fish Pathol., 37, 189−191