The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine

  • Kim, Kweon-Young (Department of Rehabilitation, College of Medicine, Chosun University) ;
  • Choi, Soo-Jin (Department of Radiology, Gacheon University Gil Medical Center) ;
  • Jang, Hyuk-Jin (Department of Psychiatry, College of Medicine, Chosun University) ;
  • Zuo, Dong-Chuan (Department of Physiology, College of Medicine, Chosun University) ;
  • Shahi, Pawan Kumar (Department of Physiology, College of Medicine, Chosun University) ;
  • Parajuli, Shankar Prasad (Department of Physiology, College of Medicine, Chosun University) ;
  • Yeum, Cheol-Ho (Department of Physiology, College of Medicine, Chosun University) ;
  • Yoon, Pyung-Jin (Department of Physiology, College of Medicine, Chosun University) ;
  • Choi, Seok (Department of Physiology, College of Medicine, Chosun University) ;
  • Jun, Jae-Yeoul (Department of Physiology, College of Medicine, Chosun University)
  • Published : 2008.06.30
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Abstract

The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at $30^{\circ}C$ and $Ca^{2+}$ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive $K^+$ channel blocker and TEA, a $Ca^{2+}$-activated $K^+$ channel blocker. Also, we found that EGCG inhibited the spontaneous $[Ca^{2+}]_i$ oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced $[Ca^{2+}]_i$ oscillations by cAMP-, cGMP-, ATP-sensitive $K^+$ channel-independent manner.

Keywords

References

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