한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제31권2호
  • /
  • Pages.195-205
  • /
  • 2008
  • /
  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

소와 돼지도체에서 Yersinia enterocolitica의 분리 및 특성

Characteristics of Yersinia enterocolitica isolates from beef and pork carcass

  • 채희선 (서울특별시 보건환경연구원) ;
  • 김주영 (서울특별시 보건환경연구원) ;
  • 김지은 (서울특별시 보건환경연구원) ;
  • 양윤모 (서울특별시 보건환경연구원) ;
  • 진경선 (서울특별시 보건환경연구원) ;
  • 신방우 (서울특별시 보건환경연구원) ;
  • 김선홍 (서울특별시 보건환경연구원) ;
  • 이정학 (서울특별시 보건환경연구원)
  • Chae, Hee-Sun (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Joo-Young (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Jee-Eun (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Yang, Yun-Mo (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Jin, Kyung-Sun (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Shin, Bang-Woo (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Kim, Sun-Heung (Seoul Metropolitan Government Research Institute of Public Health & Environment) ;
  • Lee, Jung-Hark (Seoul Metropolitan Government Research Institute of Public Health & Environment)
  • 발행 : 2008.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five (0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains (52.2%) of 23 isolates showed the multi -resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12 ($A{\sim}L$) PFGE type.

키워드

참고문헌

  1. 탁현빈. 1992. Yersinia enterocolitica의 신속증균법. 한국수의공중보건학회지 16:1-6
  2. 오호정, 김창민, 성원근 등. 1996. Polymerase chain reaction을 이용한 Yersinia enterocolitica와 Yersinia pseudotuberculosis의 조기진단법 개발에 관한 연구. 대한미생물학회지 31:165-173
  3. Falcao JP, Falcao DP, Pitondo-Silva A et al. 2006. Molecular typing and virulence markers of Yersinia entero- colitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil. J of Med Microbiol 55: 1539 -1548 https://doi.org/10.1099/jmm.0.46733-0
  4. Chiesa C, Pacifico L, Ravagnan G, 1991. Isolation of Yersinia entero- colitica serogroup O:8, biogroup 1B. J Cli Microbiol 29:2680
  5. 임순영, 윤석권. 2001. Yersinia entero- colitica의 병원성 검정에 관한 연구. 한국식품과학회지 33: 486-491
  6. Miller VL, Briska JB, Falkow S. 1990. Nucleotide sequence of the Yersinia enterocolitica ail gene and charac terization of the Ail protein product. J of Bacteriol 172 : 1062-1069 https://doi.org/10.1128/jb.172.2.1062-1069.1990
  7. National Commitee for Clinical Laboratory Standards. 2000. Performance standards for antimicrobial disk sus ceptibility test. Approved standard- seven edition 20: M100-S10
  8. Gautom RK. 1997. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organism in 1 day. J clin Microbiol 35: 2977-2980
  9. Matushek MG, Bonten MJM, Hayden MK. 1996. Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis. J Clin Microbial 34 : 2598-2600
  10. Tenover FC, Arbeit RD, Goering RV, et al. 1995. Interpreting chromosomal DNA restriction patterns produced by Pulsed-Field Gel Electrophoresis : Criteria for bacterial strain typing. Journal of Clinical Microbiology 33: 2233-2239
  11. 임순영, 이동하, 박선희 등. 1999. 식품에서 분리한 Yersinia enterocolitica의 특성조사. 한국식품과학회지 31: 183-188
  12. 조현호, 이제용, 서광욱 등. 1994. 수입돼지 및 개의 분변 유래 Yersinia entero- colitica균의 분포와 특성. I. 생물형, 혈청형 및 항생제 감수성. 한국수의공중보건학회지 18:117-125
  13. Shagegani M, Stone WB, Deforge I, et al. 1986. Yersinia enterocolitica and related species isolated from wildlife in New York State. Appl environm Microbiol 52 : 420-424
  14. Shavegani M, DeForge I, McGlynn DM, Root T. 1981. Characteristic of Yersinia enterocolitica and related species isolated from human, animal and environmental sources. J Cli Microbiol 14: 304-312
  15. Brunens AP, Frey A, Nicolet J. 1996. Association between clinical presentation, biogroups and virulence attribue of Yersinia enterocolitica strains in human diarrhoeal disease. Epidemol. Infect 116: 27-34 https://doi.org/10.1017/S0950268800058921
  16. Fenwick SG, Murray A. 1991. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction. Lancet 337: 496-497
  17. Kwaga J, Iversen JO, Misra V. 1992. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction and digoxigenin-labeled poly nucleotide probes. J Clin Microbiol 30: 2668-2673
  18. Buchrieser C, Weagant SD, Kaspar CW. 1994. Molecular characterization of Yersinia enterocolitica by pulsed- field gel electrophoresis and hybri dization of DNA fragments to ail and pYV probes. Appl Environ Microbiol 60: 4371-4379
  19. Najdenski H, Iteman I, Carniel E. 1994. Efficient subtyping of patho genic Yersinia enterocolitica strains by Pulsed-Field Gel Electrophoresis. Journal of Clinical Microbiology 32: 2913-2920
  20. Iteman I, Guiyoule A, Carniel E. 1996. Comparison of three molecular methods for typing and subtyping pathogenic Yersinia enterocolitica strains. J Med Microbiol 45:48-56 https://doi.org/10.1099/00222615-45-1-48
  21. Thisted Lambertz S, Danielsson- Tham ML, 2005. Identification and characterization of pathogenic Yersinia enterocolitica isolates by PCR and Pulsed-Field Gel Electrophoresis. Applied and Environment Microbiology 71: 3674-3681 https://doi.org/10.1128/AEM.71.7.3674-3681.2005