Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Techniques for Cryo-electron Tomography in Biological Field

생물학분야에서 Cryo-electron Tomography 활용기법

  • Mun, Ji-Young (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Kyung-Eun (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University) ;
  • Han, Sung-Sik (Laboratory of Cell Engineering & 3-D Structure, Graduate School of Life Sciences and Biotechnology, Korea University)
  • 문지영 (고려대학교 생명과학대학 생명공학원 세포 및 생체 3차 구조 연구실) ;
  • 이경은 (고려대학교 생명과학대학 생명공학원 세포 및 생체 3차 구조 연구실) ;
  • 한성식 (고려대학교 생명과학대학 생명공학원 세포 및 생체 3차 구조 연구실)
  • Published : 2008.06.30
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Abstract

In Biology, Studies Using Electron Microscopy for making Cell Structure to 3D reconstruction very fast development. Recently, by using Cryo fixation, we can see cell 3D structure without structural change, instead of using chemical fixation which can change cell structure. Before using this technology, we could understand cell structures only in 2D images. But now, through cryo-ET, 3D reconstruction of cell structure without artificial structure changes can be possible and this technology will give us many advantages in Drug delivery and Nanothechnology.

현재 생물학 분야에서 세포 구조를 3차로 구현하기 위해 전자 현미경을 이용하는 연구는 매우 빠르게 발전하고 있다. 최근에는 시료의 구조를 변형시키는 화학 고정법 대신 시료를 빠른 시간 내에 동결고정시킴으로써 구조 변형 없이 세포 3차 구조 구현이 가능해졌다. 이러한 기술의 도입으로 단편이미지로만 세포 구조를 이해해왔던 지금까지와 달리 Cryo-ET를 통해 인위적인 구조 변형 없는 3차 구조 구현이 가능해졌고, 이 기법은 약물전달, 나노 공학 등 여러 학문 분야에 활용됨으로써 학문 발전에 이바지 할 것이다.

Keywords

References

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