KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Production of Haploids from Proton Ion and Gamma-Ray Irradiation Treated $M_2$ Generation of Isolated Microspores in Brassica napus L. ssp. oleifera

앙성자 및 감마선을 처리한 유채 $M_2$ 세대의 소포자로부터 반수체 배발생

  • Kim, Kwang-Soo (Mokpo Experiment Station, National Institute of Crop Science) ;
  • Li, Mei-Yang (Mokpo Experiment Station, National Institute of Crop Science) ;
  • Jang, Young-Seok (Mokpo Experiment Station, National Institute of Crop Science) ;
  • Park, Yoon-Jung (Mokpo Experiment Station, National Institute of Crop Science) ;
  • Bang, Jin-Ki (Mokpo Experiment Station, National Institute of Crop Science)
  • 김광수 (농촌진흥청 작물과학원 목포시험장) ;
  • 이미양 (농촌진흥청 작물과학원 목포시험장) ;
  • 장영석 (농촌진흥청 작물과학원 목포시험장) ;
  • 박윤정 (농촌진흥청 작물과학원 목포시험장) ;
  • 방진기 (농촌진흥청 작물과학원 목포시험장)
  • Published : 2008.06.30
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Abstract

This experiment was carried out to investigate the effect of proton ion and gamma-ray irradiation on microspore culture of the flower buds of $M_2$ generation in winter type of Brassica napus L. ssp. oleifera. The seeds of three rape varieties, 'Halla', 'Naehan' and 'Tammi' were pretreated with proton ion and gamma-ray 400 Gy and 600 Gy, respectively. When microspore culture techniques were used, embryogenesis was increased in some varieties by proton ion and gamma-ray irradiation treated flower buds of $M_2$ generation than control. In genotypes 'Naehan' showed the highest embryo production frequency, but 'Tammi' showed lowest embryo production frequency. Some of the embryoids developed directly into plantlets, whereas others developed abnormally multilobe. Plants were regenerated and successfully acclimatized in pots.

양성자와 감마선 조사한 추파형 유채 $M_2$세대의 화뢰의 소포자 배발생에 대해 조사하였다. 세 가지 유채품종 '한라', '내한'과 '탐미' 유채종자를 각각 400 Gy와 600 Gy의 양성자와 감마선으로 전처리 하였다. 일부 종의 양성자와 감마선 조사구에서 배발생이 증가되었고 대조구보다 높았다. 품종별로는 '내한'유채가 배발생 빈도가 가장 높았고 '탐미' 유채가 가장 낮았다. 드물게 배상체로부터 바로 소식물체가 형성되었고 대부분은 비정상적으로 multilobe가 형성되었다. 배상체로부터 식물체가 성공적으로 재분화 되었고 화분에 순화되었다.

Keywords

References

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