The Controlling Activity of Several Fungicides against Rice Bakanae Disease Caused by Fusarium fujikuroi in Five Assay Methods

몇 가지 살균제의 벼 키다리병과 병원균에 대한 효과 검정

  • Shin, Myeong-Uk (Laboratory of Plant Fungal Pathogen, College of Agriculture, Life Science and Environment, Chungbuk National University) ;
  • Lee, Su-Min (Laboratory of Plant Fungal Pathogen, College of Agriculture, Life Science and Environment, Chungbuk National University) ;
  • Lee, Yong-Hwan (Plant Pathology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kang, Hyo-Jung (Chungbuk Agricultural Research and Extension Services) ;
  • Kim, Heung-Tae (Laboratory of Plant Fungal Pathogen, College of Agriculture, Life Science and Environment, Chungbuk National University)
  • 신명욱 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 이수민 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 이용환 (농업과학기술원 농업생물부) ;
  • 강효중 (충북농업기술원 시험연구부 농업환경과) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2008.06.30

Abstract

Recently damage of rice bakanae disease disseminated by infected seeds increased in paddy field in Korea. For controlling rice bakanae disease, the efficacy of 17 fungicides was assessed by 5 kinds of bioassay, spore germination test (SGT), mycelial growth test, detection test on Komada's medium (KDT), pouch test (PT) and greenhouse test (GT). Among ergosterol biosynthesis inhibiting fungicides, prochloraz showed a high controlling activity in all the assay systems while the others showed very low activity except for $500\;{\mu}g/ml$ of hexaconazole in GT and $500\;{\mu}g/ml$ of triflumizole in KDT. Although benomyl and the mixture of benomyl and thiram showed a good activity at 100 and $500\;{\mu}g/ml$ in SGT and PT, respectively, in GT they did a middle activity. Trifloxystrobin and kresoxim-methyl included in strobilurins showed a good activity even at $20\;{\mu}g/ml$ in KDT as well as a middle activity in SGT. Also a high activity not only at $10\;{\mu}g/ml$ in SGT but also at $100\;{\mu}g/ml$ KDT was detected in thiram. The activity of fludioxonil was confirmed in SGT, KDT and PT. Based on these results, it is very important to determine a bioassay system, because the fungicidal activity against rice bakanae disease was fluctuated depending on a assay systems as well as the mechanism of fungicide.

최근 국내의 벼 포장에서는 키다리병의 발생률이 크게 증가하고 있다. 본 실험에서는 키다리병 방제에 효과적으로 사용할 수 있는 살균제를 검정하고자, 5가지의 검정법을 이용하여 17종 살균제의 효과를 조사하였다. Prochloraz는 5가지의 검정법 모두에서 가장 우수한 효과를 나타내었지만, 동일한 ergosterol 생합성을 저해하는 다른 살균제 중에서 온실 검정과 Komada 배지상에서의 균검출율을 조사한 $500\;{\mu}g\;mL^{-1}$의 hexaconazole과 triflumizole을 제외하고 나머지의 효과는 매우 저조하였다. Benomyl과 benomyl/thiram의 혼합제는 100과 $500\;{\mu}g\;mL^{-1}$를 처리한 포자발아와 pouch 검정법에서 우수한 효과를 나타내었다. Trifloxystrobin과 kresoxim-methyl의 $20\;{\mu}g\;mL^{-1}$에서 소독한 종자는 균검출율이 크게 낮아졌으며, thiram 역시 $100\;{\mu}g\;mL^{-1}$의 소독 처리에서 병원균이 검출되는 종자의 비율이 감소하였다. Fludioxonil 역시 포자발아, Komada 배지에서의 균검출율, pouch 검정 등에서 우수한 효과를 보였다. 이상의 결과는 살균제의 작용기작과 특성에 맞추어 검정 방법을 결정하는 것이 중요하다는 사실을 보여주고 있다고 생각한다.

Keywords

References

  1. Bartlett, D. W., J. M. Clough, J. R. Godwin, A. A. Hall, M. Hamer and B. Parr-Dobrzanski (2002) The strobilurin fungicides. Pest Manage. Sci. 58:649-662 https://doi.org/10.1002/ps.520
  2. Buchenauer, H. (1987) Mechanism of action of triazolyl fungicides and related compounds. pp. 205-231, In Modern selective fungicides : properties, applications, mechanisms of action (ed. H. Lyr), Longman Scientific and Technical, Harlow, United Kingdom
  3. Creemers, P., J. De Vleeschauwer, D. Mappes and K. Defloor (1996) BAS 490 02 F, a new fungicide to control scab and mildew in apple and pear. Med. Fac. Landb. Gent. 61(2A). pp. 431-441
  4. Dhingra, O. D. and K. B. Sinclair, (1994) Basic plant pathology methods, 2nd ed. Lewis Publishers. pp. 369
  5. Gold, R. E., E. Ammerman, H. Koehle, G. M. E. Leinhos, G. Lorenz, J. B. Speakman, M. Stark-Umau and H. sauter (1996) The synthetic strobilurin BAS 490F: Profile of a modern fungicide. pp. 79-92. In: Modern fungicide and antifungal compounds. ed by H. Lyr, P. E. Russell and H. D. Sisler. Intercept, Andover, Hants, UK
  6. Han S. 2007. Review of disease occurrence of major crops in korea in 2007. The KSPP Annual Meeting & Symposium, pp. 19-20
  7. Johan, C. K., J. M. Richard, J. S. Donald and A, D. Maarten (1994) Inhibition of sterol biosynthesis in cell-free extracts of Botrytis cinerea by prochloraz and prochloraz analoguss. Pestic. Sci. 40:313-319 https://doi.org/10.1002/ps.2780400410
  8. Kim, H. T., S. J. Kyung, G. J. Choi, S. W. Lee and K. Y. Cho (2003) Effect of prochloraz on electrolytic leakage and spore germination of Puccinia recondita causing wheat leaf rust. 19:189-194
  9. Kato, T., S. Tanaka, S. Yamamoto, M. Ueda and Y. Kawase (1974) Effects of the fungicide, S-1358, on general metabolism and lipid biosynthesis in Monilinia fructigena. Agr. Biol. Chem. 38:2377-2384 https://doi.org/10.1271/bbb1961.38.2377
  10. Kuhlman, E. G. 1982. Varieties of Gibberella fujikuroi with anamorphs in Fusarium section Liseola. Mycologia 74, 759-768 https://doi.org/10.2307/3792862
  11. Nelson, P. E., T. A. Toussoum, and W. F. O. Marasas. (1983) Fusarium species: An illustrated manual for indentification. Penn. State. Univ. Press. p. 193
  12. Nirenberg, H. I. (1989) Identification of Fusaria occuring in Europe on cereals and potatoes. pp. 179-193. In fusarium mycotoxins, taxonomy and pathogenicity. J. Chelkowski(ed.). Elisvier. New York
  13. Sherald, J. L., N. N. Ragsdale and H. D. Sisler (1973) Similarities between the systemic fungicides triforine and triarimol. Pestic. Sci. 4:719-728 https://doi.org/10.1002/ps.2780040514
  14. Siegel, M. R. and N. N. Ragsdale (1978) Antifungal mode of action of imazalil. Pest. Biochem. Physiol. 9:48-56 https://doi.org/10.1016/0048-3575(78)90063-9
  15. Slawecki, R. A., E. P. Ryan and D. H. Young (2002) Novel fungitoxicity assays for inhibition of germination-associated adhesion of Botrytis cinerea and Puccinia recondita spores. Appl. Environ. Microbiol. 68:597-601 https://doi.org/10.1128/AEM.68.2.597-601.2002
  16. Suzuki, M., H. Hamamura and M. Iwamori (1994) Relationship between formulations of triflumizole and their efficacy to Bakanae disease in rice seed treatment. J. Pesticide Sci. 19:251-256 https://doi.org/10.1584/jpestics.19.4_251
  17. Synder, W. C. and Y. A. Toussoum. (1965) Current status of taxonomy in Fusarium species and their perfect stages. Phytopathol. 55, 833-837
  18. 김장규 (1981) 벼 키다리병의 발생생태에 관한 연구, 한국식물보호학회지 20(3):146-150
  19. 김흥태 (2002) Saccharomyces cerevisiae Y-139에서의 hexaconazole의 작용 기작. 농업과학연구 19:111-120
  20. 박흥규, 신해룡, 이인, 김석언, 권오도, 박인진, 국용인 (2003) 벼 종자소독시 수온, 처리 시간 및 약량이 벼키다리병 발병에 미치는 영향. 한국농약과학회지 7(3):216-222
  21. 성재모, 양성석, 이은종 (1984) Fusarium moniliforme의 Strain별 육묘상과 본답에서의 병 발생과 피해해석에 관한 시험. 한국균학회지 12(2):65-73
  22. 한국식물병리학회 (2004) 한국식물병명목록. 제 14판 pp. 779
  23. 황동용, 김덕수, 김상열 (2005) 키다리병 방제를 위한 종자소독 방법 연구. 한국작물학회 2005년 춘계학술발표회 요지, pp. 126-127
  24. 홍종욱, 이동진, 김장억 (1989) Prochloraz와 triadimefon의 사과나무 부란병에 대한 작용기작. 한국농화학회지 32:270-277