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알코올 급여 흰쥐의 혈중 성호르몬 및 아연 농도에 미치는 아연 고함유 효모 Saccharomyces cerevisiae 급여의 영향

Effect of Zinc-Enriched Yeast Supplementation on Serum Zinc and Testosterone Concentrations in Ethanol Feeding Rats

  • 차재영 (대선주조(주) 기술연구소) ;
  • 허진선 (동아대학교 생명자원과학대학 생명공학과) ;
  • 박보경 (동아대학교 생명자원과학대학 생명공학과) ;
  • 조영수 (동아대학교 생명자원과학대학 생명공학과)
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co. Ltd.) ;
  • Heo, Jin-Sun (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Bo-Kyung (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2008.07.30

초록

아연은 많은 생리학적인 기능을 가지는 필수미량 원소로, 특히 성기능에 있어서 중요한 역할을 하는 것으로 잘 알려져 있다. 열대 과일 Rambutan으로부터 분리된 아연 고함유 효모의 미네랄 성분은 아연 306 ppm, K 28,640 ppm, Mg 2,342, Na 1,048 ppm, Ca 366 ppm, Fe 236 ppm 및 Mn 4 ppm이 함유되어 있었다. 혈청 중 성호르몬인 테스토스테론(testosterone) 농도는 정상군에 비해 에탄올 급여 실험군에서 감소 경향을 나타내었다. 에탄올 급여에 의한 성호르몬 테스토스테론의 농도 감소는 아연 고함유 효모 투여로 현저하게 증가하였다. 또한, 혈청 중 아연 함량에 에탄올 급여 실험군에서 감소경향을 보였고, 이러한 감소는 아연 고함유 효모 투여로 현저하게 증가하였다. 따라서, 에탄올 투여에 의한 혈중 아연 및 테스토스테론 농도의 감소효과는 아연 고함유 효모 투여로 상쇄되는 것으로 나타나 본 실험에 사용된 아연 고함유 효모는 성기능 관련 건강기능식품의 소재로서 산업적으로 유용하게 사용될 것으로 사료되어 진다.

Zinc is an essential trace element participating in many physiological functions and notably an important function for sperm physiology. Zinc-enriched yeast strain, Saccharomyces cerevisiae, isolated from tropical fruit rambutan. This strain contained 306 ppm zinc concentration and other components contained by K 28,640 ppm, Mg 2,342 ppm, Na 1,048 ppm, Ca 366 ppm, Fe 236 ppm and Mn 4 ppm. The serum concentration of testosterone was decreased in ethanol treatment rats. As compared with ethanol treated control rats, the zinc-enriched yeast strain supplementation showed significantly increased the testosterone concentration in serum. In addition, zinc concentration in serum was decreased in alcohol treatment, but this reduction was significantly increased by zinc-enriched yeast strain supplementation in ethanol feeding rats. These results indicate that zinc-enriched yeast Saccharomyces cerevisiae strain could play an important role in the sperm physiology by the marked elevation of serum testosterone concentration.

키워드

참고문헌

  1. Al-Qarawi, A., A. H. Abdel-Rahman, M. S. El-Belely and S. A. El-Mougy. 2000. Age-related changes in plasma testosterone concentrations and genital organs content of bulk and trace elements in the male dromedary camel. Animal Reproduction Sci. 62, 297-307. https://doi.org/10.1016/S0378-4320(00)00146-9
  2. Assadi, F. K. and M. Ziai. 1986. Zinc status of infants with fetal alcohol syndrome. Pediatric Research 20, 551-554. https://doi.org/10.1203/00006450-198606000-00014
  3. Baltaci, A. K., R. Mogulkoc and A. Ozturk. 2006. Testosterone and zinc supplementation in castrated rats: Effects on plasma leptin levels and relation with LH, FSH and testosterone. Life Sci. 78, 746-752. https://doi.org/10.1016/j.lfs.2005.05.098
  4. Cha, J. Y., J. S. Heo and Y. S. Cho. 2008. Effect of zinc-enriched yeast FF-10 strain on the alcoholic hepatotoxicity in alcohol feeding rats. Food Sci. Biotechnol. (in press).
  5. Cha, J. Y., J. S. Heo, J. W. Kim, S. W. Lee and Y. S. Cho. 2008. Isolation and identification of zinc-enriched yeast Saccharomyces cerevisiae FF-10 from the tropical fruit rambutan. J. Life Sci. 18, 447-457. https://doi.org/10.5352/JLS.2008.18.4.447
  6. Choi, H. T., S. K. Rhee and J. Y. Son. 2001. Studies on the processing of yeast extract by waste brewery yeast. Korean J. Food Nutr. 14, 161-166
  7. Coudray, C., J. C. Tressol, C. Feillet-Coudray, J. Bellanger, D. Pepin and A. Mazur. 2000. Long-term consumption of red wine does not modify intestinal absorption or status of zinc and copper in rats. J. Nutr. 130, 1309-1313. https://doi.org/10.1093/jn/130.5.1309
  8. Diaz-Gomez, N. M., E. Doménech, F. Barroso, S. Castells, C. Cortabarria and A. Jimenez. 2003. The effect of zinc supplementation on linear growth, body composition, and growth factors in preterm infants. Pediatrics 111, 1002-1009 https://doi.org/10.1542/peds.111.5.1002
  9. Dinsmore, W., M. E. Callender, D. McMaster, S. J. Todd and A. H. Love. 1985. Zinc absorption in alcoholics using zinc-65. Digestion 32, 238-242. https://doi.org/10.1159/000199243
  10. Duncan, D. B. 1957. Multiple range test for correlated and heteroscedastic means. Biometrics 13, 164-176. https://doi.org/10.2307/2527799
  11. Elless, M., M. Blaylock, J. Huang and C. Gussman. 2000. Plants as a natural source of concentrated mineral nutritional supplements. Food Chem. 71, 181-188. https://doi.org/10.1016/S0308-8146(00)00142-4
  12. George, A. Z., S. R. Efthymia, G. T. Demetrius and A. S. John. 2002. Determination of mineral content of active dry yeast used in pharmaceutical formulations. J. Pharm. Biomedical Analysis 28, 463-473. https://doi.org/10.1016/S0731-7085(01)00601-X
  13. Goel, A., V. Dani and D. K. Dhawan. 2005. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem. Biol. Interact 156, 131-140. https://doi.org/10.1016/j.cbi.2005.08.004
  14. Hambidge, M. 2000. Human zinc deficiency. J. Nutr. 130 (Suppl), 1345-1349.
  15. Hinesly, T. D., V. Sudarski-hack, D. E. Alexander, E. L. Ziegler and G. L. Barrett. 1977. Effect of sewage sluge applications on phosphorous and metal concentrations in fractions of corn and wheat kernels. Cereal Chem. 56, 283-287.
  16. Kang, G. D., K. H. Lee, S. G. Do, C. S. Kim, J. G. Suh, Y. S. Oh and J. H. Nham. 2001. Effect of silk fibroin on the protection of alcoholic hepatotoxicity in the liver of alcohol preference mouse. Int. J. Indust. Entomol. 2, 15-18.
  17. Krebs, N. F. 2000. Overview of zinc absorption and excretion in the human gastrointestinal tract. J. Nutr. 130, 1374S-1377S. https://doi.org/10.1093/jn/130.5.1374S
  18. Kumar, N., R. P. Verma, L. P. Singh, V. P. Varshney and R. S. Dass. 2006. Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus x Bas Taurus) bulls. Reprod. Nutr. Dev. 46, 663-675. https://doi.org/10.1051/rnd:2006041
  19. Mertz, W. 1986. Zinc in Trace Elements in Human and Animal Nutrition. New York. Academic press.
  20. Motoi, H. and Y. Suzuki. 2004. A water-soluble natural zinc source prepared from defatted wheat germ. Food Sci. Biotechnol. 13, 17-20.
  21. Murillo-fuentes, M. L., R. Artillo, M. L. Ojeda, M. J. Delgado, M. L. Murillo and D. O. Carreras. 2007. Effects of prenatal or postnatal ethanol consumption on zinc intestinal absorption and excretion in rats. Alcohol Alcoholism 42, 3-10. https://doi.org/10.1093/alcalc/agl084
  22. Ohtake, Y. and Y. Okumura. 1992. Establishing a high glutathione producing yeast species. Biosci. Ind. 50, 29-34.
  23. Park, J. C., M. Ok, J. Y. Cha and Y. S. Cho. 2003. Isolation and identification of the glutathione producing Saccharomyces cerevisiae FF-8 from Korean traditional rice wine and optimal producing conditions. J. Korean Soc. Agric. Chem. Biotechnol. 46, 348-352.
  24. Prasad, A. S. 1985. Clinical and biochemical manifestations of zinc deficiency in human subjects. J. Am. Coll. Nutr. 4, 65-72. https://doi.org/10.1080/07315724.1985.10720067
  25. Prasad, A. S. 1991. Discovery of human zinc deficiency and studies in an experimental human model. Am. J. Clin. Nutr. 53, 403-412. https://doi.org/10.1093/ajcn/53.2.403
  26. Rabinovich, M., D. Figlas, S. Delmastro and N. Curvetto. 2007. Copper- and zinc-enriched mycelium of Agaricus blazei Murrill: Bioaccumulation and bioavailability. J. Med. Food 10, 175-183. https://doi.org/10.1089/jmf.2005.064
  27. Rana, S., D. Bisht and P. K. Chokraborti. 1998. Activation of rat androgen receptor by androgenic ligands is unaffected by antiandrogens in Saccharomyces cerevisiae. Gene 209, 247-254. https://doi.org/10.1016/S0378-1119(98)00054-7
  28. Rodriguez, M. F., R. E. Gonzalez, F. F. Santolaria, M. L. Galindo, T. O. Hernandez, L. N. Batista and P. M. Molina. 1997. Zinc, copper, manganese, and iron in chronic alcoholic liver disease. Alcohol 14, 39-44. https://doi.org/10.1016/S0741-8329(96)00103-6
  29. Shon, M. H., J. Y. Cha, C. H. Lee, S. H. Park and Y. S. Cho. 2007. Protective effect of administrated glutathione- enriched Saccharomyces cerevisiae FF-8 against carbon tetrachloride (CCl4)-induced hepatotoxicity and oxidative stress in rats. Food Sci. Biotechnol. 16, 967-974.
  30. Strouhal, M., R. Kizek, J. Vacek, L. Trnkova and M. Nemec. 2003. Electrochemical study of heavy metals and metallothionein in yeast Yarrowia lipolytica. Bioelectrochemistry 60, 29-36. https://doi.org/10.1016/S1567-5394(03)00043-4
  31. Tachiyashiki, K. and K. Imaizumi. 1994. Effects of dried yeast on the body weight, serum cholesterol levels, and perirenal and periepididymal adipose tissue cells in rats. J. Jpn. Sco. Nutr. Food Sci. 47, 219-226. https://doi.org/10.4327/jsnfs.47.219
  32. Take, A., K. Yano, Y. Suzuki and K. Noda. 1997. Magnesium, manganese, zinc and copper contents in Japanese foodstuffs. J. Jpn. Soc. Nutr. Food Sci. 30, 381-393.
  33. Tallman, D. L. and C. G. Taylor. 2003. Effects of dietary fat and zinc on adiposity, serum leptin and adipose fatty acid composition in C57BL/6J mice. J. Nutr. Biochem. 14, 17-23. https://doi.org/10.1016/S0955-2863(02)00228-0
  34. Vallee, B. L. and K. H. Falchuk. 1993. The biochemical basis of zinc physiology. Physiological Review 73, 79-118 https://doi.org/10.2466/pr0.1993.73.1.79
  35. Vlckova, V., S. Nadova, V. Duhova, K. Zavodan, Z. Moranova, P. Rauko, G. Kogan and E. Miadokova. 2006. Natural microbial polysaccharide sulphoethyl glucan as antigenotoxic and cancer preventing agent. Neoplasma 53, 524-529.
  36. Wilson, F. A. and A. W. Hoyumpa. 1979. Ethanol and small intestinal transport. Gastroenterology 76, 388-403.
  37. Wilson, P. and H. Greene. 2003. Importance of zinc in human nutrition. Nutr. MD 2, 1-8.
  38. Yasue, M. 2003. Brewer's yeast may prevent obesity. Bioindustry 20, 38-43.
  39. Zachariadis, G. A., E. S. Raidou, D. G. Themelis and J. A. Stratis. 2002. Determination of mineral content of active dry yeast used in pharmaceutical formulations. J. Pharm. Biomed. Anal. 28, 463-473. https://doi.org/10.1016/S0731-7085(01)00601-X

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