Differences of Antioxidative Level in the Leaves and Seed Germinability among Five Natural Populations of Hibiscus hamabo

  • Han, Sim-Hee (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Kim, Du-Hyun (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • Published : 2008.06.30

Abstract

Variations in antioxidant capacity of leaves and characteristics of seed germination among five Hibiscus hamabo populations in Korea were evaluated. While the leaves of H. hamabo from Shincheon contained highest Na concentration, those from Hado and Ohzo does lowest. MDA content and SOD activity didn't show significant difference among populations, but total protein and anthocyanin content were significantly different among five populations. The filled seed rates of five populations were ranged from 92.0 to 98.7%, and the moisture contents of seeds from Shinyang and Soando were lower than that of R. syriacus (5.18%). The fresh weights of H. hamabo from Hado and Ohzo were the highest and those of seeds from Shinyang and Soando were the lowest. Significant variations among H. hamabo populations were detected for all seed germination characters: percentage of germination, average germination time and germination rate and uniformity. SOD activities of seeds from Onpyoung and Soando were the highest of five populations, and total protein concentrations of seeds in Ohzo and Shinyang populations were higher than those of other populations. H. hamabo is considered exposed to salt stress and all characteristics of seed germinations were inferior to other species.

Keywords

References

  1. Ahn, Y.H. 2003. Distribution of native Hibiscus hamabo and ecological characteristics of naturally inhabited areas in Jeju Island. Kor. J. Hort. Sci. Technol. 21: 440-446
  2. Ahn, Y.H., K.C. Chung and H.S. Park. 2003. Vegetation and flora of Hibiscus hamabo inhabited naturally in Soan Island. J. Environ. Sci. 12: 1181-1187 https://doi.org/10.5322/JES.2003.12.11.1181
  3. Almansouri, M., J.M. Kinet and S. Lutts. 2001. Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231: 243-254 https://doi.org/10.1023/A:1010378409663
  4. Ashraf, M. and T. McNeily. 1988. Variability in salt tolerance of nine spring wheat cultivars. J. Agron. Crop Sci. 160: 14-21 https://doi.org/10.1111/j.1439-037X.1988.tb01160.x
  5. Bates, D.M. 1965. Notes on the cultivated Malvaceae. 1. Hibiscus. Baileya 13: 57-131
  6. Bazzaz, F.A., D.D. Ackerly and E.G. Reekie. 2000. Reproductive allocation in plants. In Fenner, M. (eds.) Seeds: The ecology of regeneration in plant communities. CABI Publishing, Wallingford/New York, pp. 1-29
  7. Beauchamp, C. and I. Fridovichi. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-297 https://doi.org/10.1016/0003-2697(71)90370-8
  8. Benowicz, A., R.D. Guy, M.R. Carlson and Y.A. El-Kassaby. 2001. Genetic variation among paper birch (Betula papyrifera MARSH.) populations in germination, frost hardiness, gas exchange and growth. Silvae Genet. 50: 7-13
  9. Benowicz, A., Y.A. El-Kassaby, R.D. Guy and C.C. Ying. 2000. Sitka Alder (Alnus sinuate RYDB): genetic diversity in germination, frost hardness and growth attributes. Silvae Genet. 49: 206-212
  10. Calatayud, A. and E. Barreno. 2004. Response to ozone in two lettuce varieties on chlorophyll a fluorescence, photosynthetic pigments and lipid peroxidation. Plant Physiol. and Biochem. 42: 549-555 https://doi.org/10.1016/j.plaphy.2004.05.002
  11. Davidson, D.J. and P.M. Chevalier. 1987. Influence of polyethylenglycol induced water deficits on tiller production in spring wheat. Crop Sci. 27: 1185-1187 https://doi.org/10.2135/cropsci1987.0011183X002700060019x
  12. El-Sharkawi, H.H. and F.M. Salml. 1977. Effects of drought and salinity on some growth parameters in wheat and barley. Plant Soil 46: 423-433 https://doi.org/10.1007/BF00010098
  13. Francis, F.J. 1982. Analysis of anthocyanins. In Markakis, P. (eds.) Anthocyanins as Food Colors. Academic Press, New York, pp. 195
  14. Francois, L.E., E.V. Maas, T.J. Donovan and V.L. Youngs. 1986. Effects of salinity on grain yield and quality, vegetative growth and germination of semi dwarf and drum wheat. Agron. J. 78: 1053-1058 https://doi.org/10.2134/agronj1986.00021962007800060023x
  15. Gera, M., N. Gera and H.S. Ginwal. 2000. Seed trait variations in Dalbergia sissoo Roxb. Seed Sci. Technol. 28: 467-475
  16. Hampson, C.R. and G.M. Simpson. 1990. Effects of temperature, salt and osmotic pressure on early growth of wheat (Triticum aestivum). 1. Germination. Can. J. Bot. 68: 524-528 https://doi.org/10.1139/b90-072
  17. Han, S.H., J.C. Lee, W.Y. Lee, Y. Park and C.Y. Oh. 2006. Antioxidant characteristics in the leaves of 14 coniferous trees under field conditions. J. Korean For. Soc. 95: 209-215
  18. Harper, J.L., E. Lovell and K.G. Moore. 1970. The shapes and sizes of seeds. Annu. Rev. Ecol. Syst. 1: 327-356 https://doi.org/10.1146/annurev.es.01.110170.001551
  19. Heath, R.L. and L. Parker. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189-198 https://doi.org/10.1016/0003-9861(68)90654-1
  20. Horchretiner, B.P.G. 1900. Revision De Genre Hibiscus. Ann Conserv. Jard. Bot. Geneve 4: 23-191
  21. Huh, M.R. and B.H. Kwack. 1999. Effects of uniconazole, gibberellin and calcium on salt injuries of Hibiscus syriacus and Hibiscus hamabo seedlings. J. Kor. Soc. Hort. Sci. 40: 395-398
  22. International Seed Testing Association. 1985. International rules for seed testing. Seed Sci. Technol. 13: 297-355
  23. Khajeh-Hosseini, M., A.A. Powel and I.J. Bingham. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci. Technol. 31: 715-725 https://doi.org/10.15258/sst.2003.31.3.20
  24. Kiem, D.L. and W.E. Krostad. 1981. Drought response of winter wheat cultivars grown under field stress conditions. Crop Sci. 21: 11-15 https://doi.org/10.2135/cropsci1981.0011183X002100010003x
  25. Lowry, O.H., N.J. Rosebrought, A.L. Farr and R.J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275
  26. Mamo, N., M. Mihretu, M. Fekadu, M. Tigabu and D. Teketay. 2006. Variation in seed and germination characteristics among Juniperus procera populations in Ethiopia. Forest Ecology and Management 225: 320-327 https://doi.org/10.1016/j.foreco.2006.01.026
  27. Mkonda, A., S. Lungu, J.A. Maghembe and P.L. Mafongoya. 2003. Fruit- and seed-germination characteristics of Strychnos cocculoides, an indigenous fruit tree from natural populations in Zambia. Agroforest Syst. 58: 25-31 https://doi.org/10.1023/A:1025454231002
  28. Moles, A.T. and M. Westoby. 2004. Seedling survival and seed size: a synthesis of the literature. J. Ecol. 92: 372-383 https://doi.org/10.1111/j.0022-0477.2004.00884.x
  29. Murillo-Amador, B., R. Lopez-Aguilar, C. Kaya, J. Larrinaga- Mayoral and A. Flores-Hernandez. 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. J. Agron. Crop Sci. 188: 235-247 https://doi.org/10.1046/j.1439-037X.2002.00563.x
  30. Omobuwajo, T.O., L.A. Sanni and Y.A. Balami. 2000. Physical properties of sorrel (Hibiscus sabdariffa) seeds. J. Food. Engineering 45: 37-41 https://doi.org/10.1016/S0260-8774(00)00039-X
  31. Owen, P.C.J. 1972. The relation of germination of wheat to water potential. J. Exp. Bot. 3: 188-192 https://doi.org/10.1093/jxb/3.2.188
  32. Passioura, J.B. 1988. Root signals control leaf expansion in wheat seedlings growing in drying soil. Aust. J. Plant Physiol. 15: 687-693 https://doi.org/10.1071/PP9880687
  33. Proctor, J.T.A. 1974. Color stimulation in attached apples with supplementary light. Can. J. Plant Sci. 54: 499-503 https://doi.org/10.4141/cjps74-084
  34. Siepe, T., D. Ventrella and E. Lapenta. 1997. Evaluation of genetic variability in a collection of Hibiscus cannabinus (L.) and Hibiscus spp. (L.). Industrial Crops and Products 6: 343-352 https://doi.org/10.1016/S0926-6690(97)00025-3
  35. Sivakumar, V., K.T. Parthiban, B.G. Singh, V.S. Gnanambal, R. Anandalakshmi and S. Geetha. 2002. Variability in drupe characters and their relationship on seed germination in teak (Tectona grandis L.F.). Silvae Genet. 51: 232-237
  36. Thomsen, K.A. and E.D. Kjr. 2002. Variation between single tree progenies of Fagus sylvatica in seed traits, and its implications for effective population numbers. Silvae Genet. 51: 183-190
  37. Warner, R.W. and J.E. Erwin. 2003. Effect of photoperiod and daily light integral on flowering of five Hibiscus sp. Scientia Horticulturae 97: 341-351 https://doi.org/10.1016/S0304-4238(02)00157-7
  38. Wise, D.A. and M.Y. Menzel. 1971. Genetic affinities of the north American species of Hibiscus. Sect. Trionum. Brittonia 23: 425-437 https://doi.org/10.2307/2805708
  39. Oncel, I., E. Yurdakulol, Y. Keles, L. Kurtm and A. Yildiz. 2004. Role of antioxidant defence system and biochemical adaptation on stress tolerance of high mountain and steppe plants. Acta Oecologia 26: 211-218 https://doi.org/10.1016/j.actao.2004.04.004