생약학회지 (Korean Journal of Pharmacognosy)

  • 제39권2호
  • /
  • Pages.104-109
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  • 2008
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Poncirin의 dextran sulfate sodium 유도 마우스 궤양성 대장염 증세 감소 효과

Poncirin alleviates the symptoms of dextran sulfate sodium-induced colitic mice

  • 김종빈 (경희대학교 약학대학 약품생화학교실) ;
  • 조웅 (경희대학교 약학대학 약품생화학교실) ;
  • 한아름 (이화여자대학교 약학대학 천연물화학 연구실) ;
  • 서은경 (이화여자대학교 약학대학 천연물화학 연구실) ;
  • 이경태 (경희대학교 약학대학 약품생화학교실)
  • Kim, Jong-Bin (Department of Pharmaceutical Biochemistry, College of Pharmacy) ;
  • Cho, Woong (Department of Pharmaceutical Biochemistry, College of Pharmacy) ;
  • Han, Ar-Reum (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University) ;
  • Seo, Eun-kyung (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University) ;
  • Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy)
  • 발행 : 2008.06.30
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초록

We previously reported that anti-inflammatory properties of poncirin, isolated from fruit of Poncirus trifoliata, might be the result from the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis $factor-{\acute{a}}$ ($TNF-{\alpha}$) and interlukin-6 (IL-6) expression via the down-regulation of $NF{-\kappa}B$ binding activity. In this study, we investigated whether poncirin has an inhibitory effect on the production of pro-inflammatory mediators ex vivo and whether poncirin could relieve the symptoms of dextran sulfate sodium (DSS)-induced colitis in mice model of inflammatory bowel disease. Poncirin significantly inhibited the productions of NO, IL-6 and $TNF-{\alpha}$ in lipopolysaccharide (LPS)-induced mouse peritoneal macrophage. In addition, poncirin-treated mice when compared to control mice not receiving treatment recovered better from the weight loss caused by DSS-induced colitis. Changes in disease activity index (DAI) of poncirin-treated mice were also more favorable than for control mice and were comparable with mice treated with a typical anti-inflammatory-drug, 5-aminosalichylic acid (5-ASA). In addition, suppression of plasma NO and IL-6 productions of poncirin-treated mice was also observed in DSS-induced colitis. These results suggest that poncirin has potentially useful anti-inflammatory effects mediated by suppression of inflammatory mediator productions.

키워드

참고문헌

  1. Podolsky, D. K. (2002) Inflammatory bowel disease. N. Eng. J. Med., 347: 417-429 https://doi.org/10.1056/NEJMra020831
  2. Adachi, M., Kurotani, R., Morimura, K., Shah, Y., Sanford, M., Madison, B. B., Gumucio, D. L., Marin, H. E., Peters, J. M., Young, H. A. and Gonzalez, F. J. (2006) Peroxisome proliferator activated receptor gamma in colonic epithelial cells protects against experimental inflammatory bowel disease. Gut, 55: 1104-1113 https://doi.org/10.1136/gut.2005.081745
  3. Porter, S. N., Howarth, G. S. and Butler, R. N. (1998) An orally administered growth factor extract derived from bovine whey suppresses breath ethane in colitic rats. Scand. J. Gastroenterol., 33: 967-974 https://doi.org/10.1080/003655298750027001
  4. Fan, H., Qiu, M. Y., Mei,.J. J., Shen, G. X., Liu, S. L. and Chen, R. (2005) Effects of four regulating-intestine prescriptions on pathology and ultrastructure of colon tissue in rats with ulcerative colitis. World. J. Gastroenterol., 11: 4800-4806 https://doi.org/10.3748/wjg.v11.i31.4800
  5. Eaden, J. A., Abrams, K. R. and Mayberry, J. F. (2001) The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut, 48: 526-535 https://doi.org/10.1136/gut.48.4.526
  6. Chang, D. K., Kim, Y. H., Byeon, J. S., Yang, S. K., Chung, Y. W., Han, D. S., Kim, S. G., Kim, T. I., Kim, W. H., Jeen, Y. T., Eun, C. S., Choi, H., Choi, K. Y. and Song, I. S. (2005) The current status of ulcerative colitis-associated colorectal cancer in Korea: a KASID study. Korean J Gastroenterol., 46: 276-282
  7. Hardy, R. G., Meltzer, S. J. and Jankowski, J. A. (2000) ABC of colorectal cancer. Molecular basis for risk factors. BMJ., 321: 886-889 https://doi.org/10.1136/bmj.321.7265.886
  8. Han, D. S. 대잘질환의 실험동물모델. (2002) 대한소화기학회 세미나, 125-136
  9. Ishioka, T., Kuwabara, N., Oohashi, Y. and Wakabayashi, K. (1987) Induction of colorectal tumors in rats by sulfated polysaccharides. Crit. Rev. Toxicol., 17: 215-244 https://doi.org/10.3109/10408448709071209
  10. Frank, K., Helmut, M., Marianne, A., Volker, G., Tina, B., Jürgen, S. and Josef, R. (2001) Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats. Int. J. Colorectal. Dis., 16: 238-246 https://doi.org/10.1007/s003840100311
  11. Park, S. H., Park, E. K. and Kim, D. H. (2005) Passive cutaneous anaphylaxis-inhibitory activity of flavanones from Citrus unshiu and Poncirus trifoliata. Planta Med., 71: 24-27 https://doi.org/10.1055/s-2005-837746
  12. Kim, H. M., Kim, H. J. and Park, S. T. (1999) Inhibition of immunoglobulin E production by Poncirus trifoliata fruit extract. J. Ethnopharmacol., 66: 283-288 https://doi.org/10.1016/S0378-8741(99)00028-8
  13. Liu, J. C., Chan, P., Hsu, F. L., Chen, Y. J., Hsieh, M. H., Lo, M. Y. and Lin. J. Y. (2002) The in vitro inhibitory effects of crude extracts of traditional Chinese herbs on 3-hydroxy-3-methylglutaryl-coenzyme A reductase on Vero cells. Am. J. Chinese. Med., 30: 629-636 https://doi.org/10.1142/S0192415X02000454
  14. Kim, D. H., Bae, E. A. and Han, M. J. (1999) Anti-Helicobacter pylori activity of the metabolites of poncirin from Poncirus trifoliata by human intestinal bacteria. Biol. Pharm. Bull., 22: 422-424 https://doi.org/10.1248/bpb.22.422
  15. Teng, C. M., Li, H. L., Wu, T. S., Huang, S. C. and Huang, T. F. (1992) Antiplatelet actions of some coumarin compounds isolated from plant sources. Thromb. Res., 66: 549-557 https://doi.org/10.1016/0049-3848(92)90309-X
  16. Shin, T. Y., Oh, J. M., Choi, B. J., Park, W. H., Kim, C. H., Jun, C. D. and Kim, S. H. (2006) Anti-inflammatory effect of Poncirus trifoliata fruit through inhibition of NF-kappaB activation in mast cells. Toxicol. In. Vitro., 20: 1071-1076 https://doi.org/10.1016/j.tiv.2006.02.003
  17. Han, A. R., Kim, J. B., Lee, J., Nam, J. W., Lee, I. S., Shim, C. K., Lee, K. T. and Seo, E. K. (2007) A new flavanone glycoside from the dried immature fruits of Poncirus trifoliata. Chem. Pharm. Bull (Tokyo)., 55: 1270-1273 https://doi.org/10.1248/cpb.55.1270
  18. Kim, J. B., Han, A. R., Park, E. Y., Kim, J. Y., Cho, W., Lee, J., Seo, E. K. and Lee, K. T. (2007) Inhibition of LPSinduced iNOS, COX-2 and cytokines expression by poncirin through the NF-kappaB inactivation in RAW 264.7 macrophage cells. Biol. Pharm. Bull., 30: 2345-2351 https://doi.org/10.1248/bpb.30.2345
  19. Juoekiewicz, J. and Zdunczyk, Z. (2004) Effects of cellulose, carboxymethylcellulose and inulin fed to rats as single supplements or in combinations on their caecal parameters. Comp. Biochem. Physiol. A. Mol. Integr. Physiol., 139: 513-9 https://doi.org/10.1016/j.cbpb.2004.10.015
  20. Fiocchi, C. (1998) Inflammatory bowel disease: aetiology and pathogenesis, Gastroenterology, 115: 182-205 https://doi.org/10.1016/S0016-5085(98)70381-6
  21. Sarto, R. B. (1997) Pathogenesis and immune mechanisms of chronic inflammatory bowel diseases, Am. J. Gastroenterol., 92: 5S-11S
  22. Fiocchi, C. (2004) Inflammatory bowel disease: new insights into mechanisms of inflammation and increasingly customized approaches to diagnosis and therapy. Curr. Opin. Gastroenterol., 20: 309-310 https://doi.org/10.1097/00001574-200407000-00002
  23. Chun, Y. T. and Sankawa, U. (1989) Screening of antiallergic effect in traditional medicinal drugs and active constituents of aurantii fructus imaturus. Shoyakugaku Zasshi, 43: 314-323
  24. Lee, Y. M., Kim, C. Y., Kim, Y. C. and Kim, H. M. (1997) Effects of Poncirus trifoliata on type I hypersensitivity reaction. Am. J. Chin. Med., 25: 51-56 https://doi.org/10.1142/S0192415X97000081
  25. Lee, Y. M., Kim, D. K., Kim, S. H., Shin, T. Y. and Kim, H. M. (1996) Antianaphylactic activity of Poncirus trifoliata fruit extract. J. Ethnopharmacol., 54: 77-84 https://doi.org/10.1016/S0378-8741(01)00271-9
  26. Yuan, H. D., Jhee, O. H., Kim, S. H., Kim, H. J., Om, A. S., Lee, Y. S., Baik, S. S., Kim, J. W. and Kang, J. S. (2006) The effect of curcumin on the Dextran Sulfate Sodium-induced Colitis in the Rat. Cancer. Prevention. Res., 11: 137-143
  27. Ramakers, J. D., Verstege, M. I., Thuijls. G., Te Velde, A. A., Mensink, R. P. and Plat J. (2007) The $PPAR-{\gamma}$ agonist rosiglitazone impairs colonic inflammation in mice with experimental colitis. J. Clin. Immunol., 27: 275-283 https://doi.org/10.1007/s10875-007-9074-2