Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 40 Issue 2
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- Pages.113-117
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- 2008
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
he Study of Nucleic Acid Extraction Method from Archival Paraffin Blocks
보존된 파라핀 블록에서 핵산 추출기법에 관한 연구
- Joo, Kyung-Woong (Department of Clinical Pathology, Gwangju Health College)
- 주경웅 (광주보건대학 임상병리과)
- Published : 2008.12.31
Abstract
It designed a study to examine the efficiency of DNA and RNA extraction from archival formalin-fixed, paraffin-embedded tissues using an non-heating and heating method. Archival paraffin blocks of liver, kidney, colon were randomly selected. Each paraffin block was prepared in 20 microtubes. For each paraffin blocks were tested non-heating DNA extraction to 10 microtubes and heating protocol under pH 7.0 and