The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Differential Proteomic Analysis of Secreted Proteins from Cutinase-producing Bacillus sp. SB-007

  • Ban, Yeon-Hee (School of Life Science, Chungbuk National University) ;
  • Jeon, Mi-Ri (College of Agriculture, Life & Environmental Sciences, Chungbuk National University) ;
  • Yoon, Ji-Hee (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Park, Jae-Min (School of Life Science, Chungbuk National University) ;
  • Um, Hyun-Ju (School of Life Science, Chungbuk National University) ;
  • Kim, Dae-Soon (School of Life Science, Chungbuk National University) ;
  • Jung, Seung-Ki (Bioresource Inc.) ;
  • Kim, Keun-Young (Bioresource Inc.) ;
  • Lee, Jee-Won (Department of Chemical and Biological Engineering, Korea University) ;
  • Min, Ji-Ho (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Kim, Yang-Hoon (School of Life Science, Chungbuk National University)
  • 발행 : 2008.06.30
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초록

Bacillus sp. SB-007 was isolated from pea leaves harvested from the southwestern parts of South Korea through screening on a minimal medium containing 0.2% purified cutin for its ability to induce the cutinase production. However, no cutinase was produced when it was grown in a minimal medium containing 0.2% glucose. A proteomic approach was applied to separate and characterize these differentially secreted proteins. The expression level of 83 extracellular proteins of the cutinase-producing Bacillus sp. strain SB-007 incubated in a cutinase-induced medium increased significantly as compared with that cultured in a non cutinase-induced medium containing glucose. The extracellular proteome of Bacillus sp. SB-007 includes proteins from different functional classes, such as enzymes for the degradation of various macromolecules, proteins involved in energy metabolism, sporulation, transport/binding proteins and lipoproteins, stress inducible proteins, several cellular molecule biosynthetic pathways and catabolism, and some proteins with an as yet unknown function. In addition, the two protein spots showed little similarities with the known lipolytic enzymes in the database. These secreted proteome analysis results are expected to be useful in improving the Bacillus strains for the production of industrial cutinases.

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참고문헌

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