Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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Temperature Controllable HPLC Column for Preparative Fractionation of Polymers

  • Im, Kyu-Hyun (Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology) ;
  • Park, Hae-Woong (Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology) ;
  • Kim, Young-Tak (Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology) ;
  • Chang, Tai-Hyun (Department of Chemistry and Polymer Research Institute, Pohang University of Science and Technology)
  • Published : 2008.08.31
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Abstract

An HPLC column with a self-contained temperature control device was constructed for preparative temperature programmed interaction chromatography. Two Peltier plates were attached to a large bore column ($120{\times}22\;mm$ i.d.) and the column temperature was controlled by PID mode feed back control. At a flow rate of 1.5 mL/min, the column temperature could be increased and decreased at a rate as high as $50^{\circ}C/min$ and $10^{\circ}C/min$, respectively, which is much faster than using a column jacket and bath/circulator. The rapid heating and cooling rates allows a high repetition rate of chromatographic fractionation. The performance of the temperature controllable column was demonstrated successfully by the fractionation of homo-polymer precursors from diblock copolymers.

Keywords

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