Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Shape of Synaptic Vesicles in the Tooth Pulp Afferent Terminals and P-endings in the Rat Trigeminal Nucleus Principalis

흰쥐의 삼차신경주감각핵에서 치수유래 일차들신경종말 및 이와 연접하는 연접이전신경종말의 연접소포 형태 비교

  • Kim, Yun-Sook (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University) ;
  • Paik, Sang-Kyoo (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University) ;
  • Kwak, Woo-Kyung (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University) ;
  • Cho, Yi-Sul (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University) ;
  • Kim, Ji-Man (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University) ;
  • Park, Mae-Ja (Department of Anatomy, School of Medicine, Brain Korea 21, Kyungpook National University) ;
  • Bae, Yong-Chul (Department of Oral Anatomy and Neurobiology, School of Dentistry, Brain Korea 21, Kyungpook National University)
  • 김윤숙 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21) ;
  • 백상규 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21) ;
  • 곽우경 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21) ;
  • 조이슬 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21) ;
  • 김지만 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21) ;
  • 박매자 (경북대학교 의학전문대학원 해부학교실, Brain Korea 21) ;
  • 배용철 (경북대학교 치의학전문대학원 구강해부학교실, Brain Korea 21)
  • Published : 2008.12.31
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Abstract

In order to investigate shape of synaptic vesicles of the tooth pulp afferent boutons and their presynaptic endings (p-endings), and the neuroactive substance of the p-endings in the trigeminal nucleus principalis, rat incisor tooth pulp afferents were labeled by the horseradish peroxidase (HRP) and quantitative ultrastructural analysis and postembedding immunogold labeling were performed. Labeled tooth pulp afferent boutons contained clear, spherical synaptic vesicles (diameter: $45{\sim}55\;nm$) and occasionally dense core vesicles(diameter: $80{\sim}120\;nm$). They formed symmetrical synapses with unlabeled axon terminals (p-endings) containing pleomorphic synaptic vesicles. The ratio of short to long diameter (form factor) of synaptic vesicles of pulp afferent boutons was 0.6 to 0.99, whereas that of p-endings was 0.25 to 0.99. In addition, most of the p-endings showed GABA-like immunoreactivity. These results indicate that the shape of synaptic vesicles is quite different between the tooth pulp afferent boutons and p-endings, and the p-endings may contain GABA as a neuroactive substance in the trigeminal nucleus principalis.

삼차신경주감각핵에서 치수유래 일차들신경섬유의 종말과 그에 연접하는 연접이전신경종말에 함유된 연접소포들을 형태학적으로 비교하기 위하여 신경추적자(WGA-HRP)를 흰쥐 앞니의 치수강에 주입하여 치수 유래 일차들신경종말을 표식한 후, 투과형 전자현미경을 통한 미세구조적 정량 분석을 실시하였다. 표식된 치수유래 일차들신경종말은 직경이 $45{\sim}55\;nm$인 구형의 소포를 함유하고 있었으며, 일부 직경이 $80{\sim}120\;nm$ 큰 치밀소포가 관찰되기도 하였다. 또한 표식된 신경종말은 다형성 연접소포를 함유하고 있는 연접이전신경종말과 대칭연접을 이루고 있었다. 일차들신경종말의 연접소포들은 긴 지름에 대한 짧은 지름의 비율(form factor)이 $0.6{\sim}0.99$의 분포를 보인 반면, 연접이 전신경종말의 연접소포들은 $0.25{\sim}0.99$까지 다양하게 나타났다. 또한 대부분의 연접이전신경종말은 GABA에 대한 면역양성반응을 보였다. 이상의 결과는 표식된 신경종말과 그에 연접하는 연접이전신경종말의 연접소포는 서로 다른 형태를 보이고 있으며, 또한 그 연접이전신경종말이 억제성 신경전달물질인 GABA를 함유하고 있음을 나타낸다.

Keywords

References

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