한국수소및신에너지학회논문집 (Journal of Hydrogen and New Energy)

  • 제19권1호
  • /
  • Pages.41-48
  • /
  • 2008
  • /
  • 1738-7264(pISSN)
  • /
  • 2288-7407(eISSN)
한국수소및신에너지학회 (The Korean Hydrogen and New Energy Society)
권호 표지

하수슬러지로부터 분리된 혐기세균에 의한 수소생산 최적화

Optimization of Hydrogen Production Using the Mixed Microflora Isolated from Sewage Sludge

  • 오유관 (한국에너지기술연구원 바이오에너지연구센터) ;
  • 김미선 (한국에너지기술연구원 바이오에너지연구센터)
  • Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research)
  • 발행 : 2008.02.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Fermentative $H_2$ production was studied using microbial consortia isolated from heat-treated ($90{\circ}C$, 20 min) sewage sludge. Important parameters investigated were carbon(C) and nitrogen(N)-sources, C/N ratio, phosphate concentration, pH and temperature during anaerobic cultivation in serum bottles. Starch, ribose, sucrose and glucose were good C-sources for the culture growth and $H_2$ production. Yeast extract was better N-source than $(NH_4)_2SO_4$ or peptone when individually added to the synthetic media, however the combination of above three N-sources exhibited the additional effect for cell growth and $H_2$ evolution. Addition of 100 mM phosphate as a buffering agent prevented the rapid pH drop during the cultivation. The optimum initial pH for the cell growth was at 7.0, whereas $H_2$ production was observed at pH 5.5. Optimum temperature for the cell growth and $H_2$ production was $37{\circ}C$. Initial C/N ratio of 1.22 in the media using glucose and yeast extract as the C- and N-sources, respectively, showed the $H_2$ yield 1.0 mol $H_2$/mol glucose.

키워드

참고문헌

  1. H. Koku, I. Erolu, U. Gunduz, M. Yucel, and L. Turker, 'Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides', Int. J. Hydrogen Energy, Vol. 27, 2002, pp. 1315-1329 https://doi.org/10.1016/S0360-3199(02)00127-1
  2. D. Das and T. N. Veziroglu, 'Hydrogen production by biological processes', Int J. Hydrogen Energy, Vol. 26, 2001, pp. 13-28 https://doi.org/10.1016/S0360-3199(00)00058-6
  3. L. J. Stal and R. Moezelaar, 'Fermentation in cyanobacteria', FEMS Microbiol. Rev., Vol. 21, 1997, pp. 179-211 https://doi.org/10.1016/S0168-6445(97)00056-9
  4. J.-H. Shin and T. H. Park, 'Biological hydrogen production process', Korean Chem. Eng. Res., Vol. 44, 2006, pp. 16-22
  5. M. Heyndrickx, P. De Vos, and J. De Ley, 'Fermentation characteristics of Clostridium pasteurianum LMG 3285 grown on glucose and mannitol', J. Appl. Bacteriol. Vol. 70, 1991, pp. 52-58 https://doi.org/10.1111/j.1365-2672.1991.tb03786.x
  6. H. P. P Herber and H. Liu, 'Effect of pH on hydrogen production from glucose by a mixed culture', Bioresource Technol., Vol. 82, 2002, pp. 87-93 https://doi.org/10.1016/S0960-8524(01)00110-9
  7. C. Collet, N. Adler, J. P. Schwitzguebel, and P. Peringer, 'Hydrogen production by Clostridium thermolacticum during continuous germentation of lactose', Int. J. Hydrogen Energy, Vol 29, 2004, pp. 1479-1485 https://doi.org/10.1016/j.ijhydene.2004.02.009
  8. I. -S. Chang, P. Le Clech, B. Jefferson, and S. Judd, 'Membrane fouling in membrane bioreactors for wastewater treatment', J. Envir. Engrg, Vol. 128, 2002, pp. 1018-1029 https://doi.org/10.1061/(ASCE)0733-9372(2002)128:11(1018)
  9. C. Y. Lin and R. C. Chang, 'Hydrogen production during the anaerobic acidogenic conversion of glucose', J. Chem. Technol. Biotechnol., Vol. 74, 1999, pp. 498-500 https://doi.org/10.1002/(SICI)1097-4660(199906)74:6<498::AID-JCTB67>3.0.CO;2-D
  10. B. K. Ahring, K. Jensen, P. Nielsen, A. B. Bjerre, and A. S. Schmid, 'Pretreatment of wheat straw and conversion of xylose and xylan to ethanol by thermophilic anaerobic bacteria', Bioresource Technol, Vol. 58, 1996, pp. 107-113 https://doi.org/10.1016/S0960-8524(96)00090-9
  11. C. Y. Lin and C. H. Lay, 'Carbon/nitrogenratio effect on fermentative hydrogen production by mixed microflora', Int. J. Hydrogen Energy, Vol. 29, 2004, pp. 41-45 https://doi.org/10.1016/S0360-3199(03)00083-1
  12. O. Mizno, R. Dinsdale, F. R. Hawkes, D. L. Hawkes, and T. Noike, 'Enhancement of hydrogen production from glucose by nitrogen gas sparging', Bioresource Technol., Vol. 73, 2000, pp. 59-65 https://doi.org/10.1016/S0960-8524(99)00130-3
  13. F. R. Hawkes, R. Dinsdale, D. L. Hawkes, and I. Hussy, 'Sustainable fermentative hydrogen production, challenges for process optimisation', Int. J. Hydrogen energy, Vol. 27, 2002, pp. 1339-1347 https://doi.org/10.1016/S0360-3199(02)00090-3
  14. S.-E. Oh, P. Iyer, M. A. Bruns, and B. E. Logan, 'Biological hydrogen production using a membrane bioreactor', Biotechnol. Bioeng., Vol. 87, 2004, pp. 119-127 https://doi.org/10.1002/bit.20127
  15. K. S. Lee, C. M. Kang, and S. Y. Chung, 'Medium composition of Enterobacter cloacae YJ-1 for maximizing hydrogen production', Korean J. Biotechnol. Bioeng., Vol. 20, 2005, pp. 350-354
  16. E. W. J. van Niel, P. A. M. Claassen, and A. J. M. Stams, 'Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus', Biotechnol. Bioeng., Vol. 81, pp. 255-262 https://doi.org/10.1002/bit.10463